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Abstract

Introduction:Oxidative stress may lead to a number of diseases such as atherosclerosis, nephrotoxicity, liver cirrhosis, cancers, diabetes, and Alzheimer disease. Medicinal plants are an important source of antioxidants. Therefore, the antioxidant potential of Dillenia bracteata was evaluated in the present study. Materials and Methods: The coarse powder of leaf of Dillenia bracteata (Dilleniaceae) was extracted in Soxhlet apparatus, with ethanol (90%) and ethanolic extract of D. bracteata (EEDB) was further processed for phytochemical screening, total phenol content, total flavonoid content, and various in vitro antioxidant assays. Results and Discussion: The phytochemicals present in EEDB were glycosides, carbohydrates, triterpenoids, proteins and amino acids, gums and mucilages, and flavonoids. The total phenolic and flavonoid contents of EEDB were found to be 168.67 mg gallic acid equivalents per gram of dry extract and 61.67 mg quercetin equivalents per gram of dry extract, respectively. In 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, EEDB exerted an inhibition of 74% and ascorbic acid exerted 83% at 500 μg/ml. In the ferric reducing antioxidant power assay, the absorbance of EEDB increased with increasing concentration. Hydroxyl radical scavenging activity of EEDB was 67% and ascorbic acid reached 74% at 500 μg/ml. The superoxide radical scavenging activity of EEDB was 87%, and ascorbic acid reached 89% at 1000 μg/ml. Nitric oxide radical scavenging activity of EEDB reached 69.21% and ascorbic acid reached 84.01% at 500 μg/ml. Conclusion: The antioxidant effect of D. bracteata leaf may be due to the phenolics and flavonoids present in it. The present study suggests that this extract could be of great importance for the treatment of free radical-related diseases.

Phytochemical screening and antioxidant activity of methanol extract of Dillenia excelsa leaf

“…Ethanol extract of D. indica has an inhibition percentage of 97.10±0.01% (Saha et al 2009;Rahayu et al 2019). Ethanolic extract of D. bracteata leaves inhibited 74% with DPPH (Shama et al 2019). Patel et al (2020 reported that the ethanolic extract of D. pentagyna leaves has an inhibition percentage of 76.46± 1.58% with the DPPH method.…”

“…Thooptianrat et al (2017); Putra et al (2019); Yakop et al (2020) showed that ethyl acetate extract of D. suffruticosa leaves contains tannins, polyphenols, and triterpenoids and triterpenoids. On the other hand, Shama et al (2019) reported that the ethanolic extract of D. bracteata contains glycosides, carbohydrates, triterpenoids, proteins, amino acids, gums, mucilages, and flavonoids.…”

“…The total phenolic content of the bark extract of D. auriculata has total phenolics equivalent to 19.70±0.05 GAE/g extract (Liana and Murningsih 2019). Ethanolic extract of D. bracteata leaves has 68.67 mg GAE/g extract (Shama et al 2019), while the total phenolic concentration of stem bark D. serrata was 59.2 mg GAE/g (Sabandar et al 2020). Methanol extract D. suffruticosa leaves has total phenolic content of 309.91 ±3.42 mgGAE/g dry weight extract (Yakop et al 2020).…”

“…Rahayu et al (2019) reported that ethanol extract of D. indica leaves contains a flavonoid content of 2.953 mg (QE/mg) in macerated 24 hours at 15% concentration. Ethanolic extract of D. bracteata leaves has 61.67 mg quercetin equivalents per g of dry extract (Shama et al 2019). While total flavonoid content in the stem bark extract D. serrata was 23.4 mg QE/g (Sabandar et al 2020).…”

“…While, the ethanolic extract of Dillenia pentagyna contains phenolics, flavonoids, tannins, saponins, alkaloids, and terpenoids (Patle et al 2020), and potentially as anticancer, antidiarrheal, antimicrobial, antioxidant (Ghandi and Mehta 2013). On the other hand, extract ethanolic Dillenia bracteata contains glycosides, carbohydrates, triterpenoids, proteins, amino acids, gums, mucilages, and flavonoids, antioxidants potentially (Shama et al 2019).…”