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Allaway, David; Schofield, Neil A.; Poole, Philip S.

doi:10.1023/a:1005647711606

Cited by 10 publications

(1 citation statement)

References 35 publications

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“…Mehta et al 4 gave a detailed review of an investigation of single-molecule biomechanics using optical methods. Allaway et al 5 provided a short review of the application of optical trapping over a wide spectrum of biological research. Zhang and Liu 6 gave an updated review of the extensive body of work in the field of single-cell studies using optical tweezers.…”

Section: Introductionmentioning

confidence: 99%

Survey on indirect optical manipulation of cells, nucleic acids, and motor proteins

et al. 2011

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Optical tweezers have emerged as a promising technique for manipulating biological objects. Instead of direct laser exposure, more often than not, optically-trapped beads are attached to the ends or boundaries of the objects for translation, rotation, and stretching. This is referred to as indirect optical manipulation. In this paper, we utilize the concept of robotic gripping to explain the different experimental setups which are commonly used for indirect manipulation of cells, nucleic acids, and motor proteins. We also give an overview of the kind of biological insights provided by this technique. We conclude by highlighting the trends across the experimental studies, and discuss challenges and promising directions in this domain of active current research.

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Section: Introductionmentioning

confidence: 99%

Survey on indirect optical manipulation of cells, nucleic acids, and motor proteins

et al. 2011

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Cellular Manipulations

Gonzalez

2003

Advanced Semiconductor and Organic Nano-Techniques

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Use of differential fluorescence induction and optical trapping to isolate environmentally induced genes

Allaway

Schofield

Leonard

et al. 2001

Environmental Microbiology

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The techniques of differential fluorescence induction (DFI) and optical trapping (OT) have been combined to allow the identification of environmentally induced genes in single bacterial cells. Designated DFI-OT, this technique allows the in situ isolation of genes driving the expression of green fluorescent protein (Gfp) using temporal and spatial criteria. A series of plasmid-based promoter probe vectors (pOT) was developed for the construction of random genomic libraries that are linked to gfpUV or egfp. Bacteria that do not express Gfp on laboratory medium (i.e. non-fluorescent) were inoculated into the environment, and induced genes were detected with a combined fluorescence/optical trapping microscope. Using this selection strategy, rhizosphere-induced genes with homology to thiamine pyrophosphorylase (thiE) and cyclic glucan synthase (ndvB) were isolated. Other genes were expressed late in the stationary phase or as a consequence of surface-dependent growth, including fixND and metX, and a putative ABC transporter of putrescine. This strategy provides a unique ability to combine spatial, temporal and physical information to identify environmental regulation of bacterial gene expression.

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Untitled

Cited by 10 publications

References 35 publications

Survey on indirect optical manipulation of cells, nucleic acids, and motor proteins

Survey on indirect optical manipulation of cells, nucleic acids, and motor proteins

Cellular Manipulations

Use of differential fluorescence induction and optical trapping to isolate environmentally induced genes

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