Untitled

Attal, J.; Théron, Marie-Claire; Rival, Sylvie; Puissant, C.; Houdebiné, Louis‐Marie

doi:10.1023/a:1007084730470

Cited by 10 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…*, P Ͻ 0.05. modules can give rise to artificial IRES activity (40). Furthermore, when the R region of the HTLV-1 5=UTR is attached to other genetic elements, it is known to stimulate gene expression (41,42). To rule out the possibility that the ⌬EMCV sequence together with the HTLV-1 5=UTR could artificially exhibit IRES activity, the luciferase activities from bicistronic reporters containing the ⌬EMCV sequences (dl HTLV-1 5=UTR) or not (dl ⌬⌬EMCV HTLV-1 5=UTR) were measured (Fig.…”

Section: Resultsmentioning

confidence: 99%

The 5′ Untranslated Region of the Human T-Cell Lymphotropic Virus Type 1 mRNA Enables Cap-Independent Translation Initiation

Olivares

Landry

Cáceres

et al. 2014

J Virol

View full text Add to dashboard Cite

The human T-cell leukemia virus type 1 (HTLV-1) is a complex human retrovirus that causes adult T cell leukemia and of HTLVassociated myelopathy/tropical spastic paraparesis. The mRNA of some complex retroviruses, including the human and simian immunodeficiency viruses (HIV and SIV), can initiate translation using a canonical cap-dependent mechanism or through an internal ribosome entry site (IRES). In this study, we present strong evidence showing that like HIV-1 and SIV, the 5=-untranslated region (5=UTR) of the HTLV-1 full-length mRNA harbors an IRES. Cap-independent translational activity was evaluated and demonstrated using dual luciferase bicistronic mRNAs in rabbit reticulocyte lysate, in mammalian cell culture, and in Xenopus laevis oocytes. Characterization of the HTLV-1 IRES shows that its activity is dependent on the ribosomal protein S25 (RPS25) and that its function is highly sensitive to the drug edeine. Together, these findings suggest that the 5=UTR of the HTLV-1 full-length mRNA enables internal recruitment of the eukaryotic translation initiation complex. However, the recognition of the initiation codon requires ribosome scanning. These results suggest that, after internal recruitment by the HTLV-1 IRES, a scanning step takes place for the 40S ribosomal subunit to be positioned at the translation initiation codon. IMPORTANCEThe mechanism by which retroviral mRNAs recruit the 40S ribosomal subunit internally is not understood. This study provides new insights into the mechanism of translation initiation used by the human T-cell lymphotropic virus type 1 (HTLV-1). The results show that the HTLV-1 mRNA can initiate translation via a noncanonical mechanism mediated by an internal ribosome entry site (IRES). This study also provides evidence showing the involvement of cellular proteins in HTLV-1 IRES-mediated translation initiation. Together, the data presented in this report significantly contribute to the understanding of HTLV-1 gene expression.

show abstract

Section: Resultsmentioning

confidence: 99%

The 5′ Untranslated Region of the Human T-Cell Lymphotropic Virus Type 1 mRNA Enables Cap-Independent Translation Initiation

Olivares

Landry

Cáceres

et al. 2014

J Virol

View full text Add to dashboard Cite

show abstract

“…This study used full length cDNA sequences for the expression of Cxs, including untranslated regions (UTRs), rather than purely coding sequence. Intercistronic length has been shown to affect IRES-dependent bicistronic expression with greater intercistronic length adversely affecting translation [17]. Both Cx sequences contain large 3' UTRs contributing to intercistronic length that may interfere with IRES-dependent translation.…”

Section: Fig 3 Near Herementioning

confidence: 99%