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Vershinina, O. A.; Znamenskaya, L. V.

doi:10.1023/a:1020547616096

Cited by 88 publications

(20 citation statements)

References 107 publications

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“…It is not surprising that low AP conditions favor the activity of phosphatases (Nannipieri et al, 2011). When facing P scarcity, microorganisms can upregulate the expression of the specific functional genes that encode phosphatases (Vershinina and Znamenskaya, 2002). Additionally, both TN and TC as substrates contribute to the growth of various PSM species (Nahas, 2007).…”

Section: Discussionmentioning

confidence: 99%

Soil Nutrients Drive Function and Composition of phoC-Harboring Bacterial Community in Acidic Soils of Southern China

Zheng

Wang

et al. 2019

Front. Microbiol.

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Phosphorus (P) deficiency is an important factor that limits the agricultural production potential in acidic soils. The bacterial phoC gene encodes non-specific acid phosphatase (ACP), which participates in the mineralization of soil organic P and is therefore important for the improvement of soil P availability. However, the function and community population of phoC-harboring bacteria and their driving factors in acidic soil remain largely unknown. For this study, 51 soil samples and 207 plant samples were collected from four locations in the acidic soil region of southern China. Quantitative PCR and high-throughput sequencing were employed to analyze abundance and community composition of phoC-harboring bacteria. The results showed that soil P availability was the important nutrient element limiting the growth of both plants and soil bacteria. Soil ACP activity was clearly higher than alkaline phosphatase, indicating the important effect of phoC-harboring bacteria in acidic soils. ACP activity and phoC gene abundance showed a significant positive correlation, and both were closely related to soil available P, total carbon, and total nitrogen. The dominant genera of phoC-harboring bacteria involved Cupriavidus, Stenotrophomonas, and Xanthomonas. Compared to land-use pattern, sampling location, and soil parent material, soil property played a more important role in affecting phoC-harboring bacterial community structure, where N-related variables including soil NO3- -N, NH4+ -N, and C/N ratio appeared to be the main factors. These findings suggest that phoC-harboring bacteria should provide an important contribution to soil P availability in acidic soil, and its function and community composition were strongly associated with soil nutrients.

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Section: Discussionmentioning

confidence: 99%

Soil Nutrients Drive Function and Composition of phoC-Harboring Bacterial Community in Acidic Soils of Southern China

Zheng

Wang

et al. 2019

Front. Microbiol.

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“…Some microbes contain phosphate starvation-inducible (psi) genes and act as part of the phosphate starvation regulon. These microbes have the capacity to synthesize phosphomonoesterases and phosphodiesterase (Vershinina and Znamenskaya, 2002). The Oxalobacteraceae (mainly Massilia and Herbaspirillum), Klebsiella, and some species of Burkholderia and Bacillus were enriched in rock phosphate-amended soil compared to triple superphosphatetreated soil.…”

Section: Introductionmentioning

confidence: 99%

Rhizoplane Bacteria and Plant Species Co-determine Phosphorus-Mediated Microbial Legacy Effect

Lang

Bei

et al. 2019

Front. Microbiol.

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“…Microorganisms can access and recycle P from these recalcitrant P forms by solubilization of inorganic P and by mineralization of organic P via enzymatic processes mediated primarily by phosphatases, which hydrolyze the orthophosphate group from organic compounds (3). When facing P scarcity, microorganisms upregulate expression of functional genes coding for phosphatases (phosphomonoesterases, phosphodiesterases, phytases), high-affinity phosphate transporters, and enzymes for phosphonate utilization, which together constitute the Pho regulon (4). The phosphomonoesters which are hydrolyzed by phosphatases are generally the dominant fraction of organic P and can represent up to 90% of the organic P in soil (3).…”

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phoD Alkaline Phosphatase Gene Diversity in Soil

Ragot

Kertesz

Bünemann

2015

Appl Environ Microbiol

254

151

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bPhosphatase enzymes are responsible for much of the recycling of organic phosphorus in soils. The PhoD alkaline phosphatase takes part in this process by hydrolyzing a range of organic phosphoesters. We analyzed the taxonomic and environmental distribution of phoD genes using whole-genome and metagenome databases. phoD alkaline phosphatase was found to be spread across 20 bacterial phyla and was ubiquitous in the environment, with the greatest abundance in soil. To study the great diversity of phoD, we developed a new set of primers which targets phoD genes in soil. The primer set was validated by 454 sequencing of six soils collected from two continents with different climates and soil properties and was compared to previously published primers. Up to 685 different phoD operational taxonomic units were found in each soil, which was 7 times higher than with previously published primers. The new primers amplified sequences belonging to 13 phyla, including 71 families. The most prevalent phoD genes identified in these soils were affiliated with the orders Actinomycetales (13 to 35%), Bacillales (1 to 29%), Gloeobacterales (1 to 18%), Rhizobiales (18 to 27%), and Pseudomonadales (0 to 22%). The primers also amplified phoD genes from additional orders, including Burkholderiales, Caulobacterales, Deinococcales, Planctomycetales, and Xanthomonadales, which represented the major differences in phoD composition between samples, highlighting the singularity of each community. Additionally, the phoD bacterial community structure was strongly related to soil pH, which varied between 4.2 and 6.8. These primers reveal the diversity of phoD in soil and represent a valuable tool for the study of phoD alkaline phosphatase in environmental samples. P hosphorus (P) is an essential macronutrient for all living cells (1). Despite its relative abundance in soils, P is one of the main limiting nutrients for terrestrial organisms (2). P is present in organic and inorganic forms in soil, but only the inorganic orthophosphate ions in soil solutions are readily available for plants (3). To sustain crop productivity, large amounts of P fertilizers are therefore used in agriculture, both as inorganic fertilizers (e.g., triple super phosphate) and organic fertilizers (e.g., manure). After application, some of the inorganic P is rapidly taken up by plants and microorganisms, while the remaining P is immobilized as insoluble and bound P forms in the soil. Microorganisms can access and recycle P from these recalcitrant P forms by solubilization of inorganic P and by mineralization of organic P via enzymatic processes mediated primarily by phosphatases, which hydrolyze the orthophosphate group from organic compounds (3). When facing P scarcity, microorganisms upregulate expression of functional genes coding for phosphatases (phosphomonoesterases, phosphodiesterases, phytases), high-affinity phosphate transporters, and enzymes for phosphonate utilization, which together constitute the Pho regulon (4). The phosphomonoesters which are hydrolyzed ...

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Untitled

Cited by 88 publications

References 107 publications

Soil Nutrients Drive Function and Composition of phoC-Harboring Bacterial Community in Acidic Soils of Southern China

Soil Nutrients Drive Function and Composition of phoC-Harboring Bacterial Community in Acidic Soils of Southern China

Rhizoplane Bacteria and Plant Species Co-determine Phosphorus-Mediated Microbial Legacy Effect

phoD Alkaline Phosphatase Gene Diversity in Soil

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