Untitled

Landers, Rüdiger; Pfister, Andreas; Hübner, U.; Hardy, John; Schmelzeisen, Rainer; Mülhaupt, Rolf

doi:10.1023/a:1016189724389

Cited by 337 publications

(126 citation statements)

References 8 publications

Supporting

Mentioning

126

Contrasting

Order By: Relevance

“…4 The most commonly used bioprinting techniques are extrusion-based, particle fusion-based, light-induced, and inkjet-based bioprinting. [1][2][3][5][6][7][8][9] Extrusion-based bioprinting is one of the most popular techniques due to compatibility with a variety of bioink, ease of operation and relatively low cost. 3,10 Among various bioinks, hydrogels represent a class of promising materials because they provide a highly hydrated, biocompatible, 3D environment.…”

Section: Introductionmentioning

confidence: 99%

3D bioprinting of liver-mimetic construct with alginate/cellulose nanocrystal hybrid bioink

Lin²,

et al. 2018

View full text Add to dashboard Cite

Abstract3D bioprinting is a novel platform for engineering complex, three-dimensional (3D) tissues that mimic real ones. The development of hybrid bioinks is a viable strategy that integrates the desirable properties of the constituents. In this work, we present a hybrid bioink composed of alginate and cellulose 2 nanocrystals (CNCs) and explore its suitability for extrusion-based bioprinting. This bioink possesses excellent shear-thinning property, can be easily extruded through the nozzle, and provides good initial shape fidelity. It has been demonstrated that the viscosities during extrusion were at least two orders of magnitude lower than those at small shear rates, enabling the bioinks to be extruded through the nozzle (100 μm inner diameter) readily without clogging. This bioink was then used to print a liver-mimetic honeycomb 3D structure containing fibroblast and hepatoma cells. The structures were crosslinked with CaCl 2 and incubated and cultured for 3 days. It was found that the bioprinting process resulted in minimal cell damage making the alginate/CNC hybrid bioink an attractive bioprinting material.Graphical abstract

show abstract

Section: Introductionmentioning

confidence: 99%

3D bioprinting of liver-mimetic construct with alginate/cellulose nanocrystal hybrid bioink

Lin²,

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The main problem with fabricating such a scaffold is that the islet is approximately 100-200 µm, which means that the scaffold pore sizes must be 5-10 times that diameter (63); thus, it is appropriate to investigate other fabrication methods such as rapid prototyping techniques that use computer-aided design (CAD) models to create the desired scaffold with controlled geometries (63,129). Conventional scaffold fabrication techniques, such as freeze-drying, do not allow for controlled pore structure; moreover, larger pore sizes required for islet culture would render the resulting scaffold unstable.…”

Section: Encapsulationmentioning

confidence: 99%

Pancreatic Islet Culture and Preservation Strategies: Advances, Challenges, and Future Outlook

2010

View full text Add to dashboard Cite

Postisolation islet survival is a critical step for achieving successful and efficient islet transplantation. This involves the optimization of islet culture in order to prolong survival and functionality in vitro. Many studies have focused on different strategies to culture pancreatic islets in vitro through manipulation of culture media, surface modified substrates, and the use of various techniques such as encapsulation, embedding, scaffold, and bioreactor culture strategies. This review aims to present and discuss the different methodologies employed to optimize pancreatic islet culture in vitro as well as address their respective advantages and drawbacks.

show abstract

“…The scaffolds were fabricated using a bioplotter from EnvisionTec, which is essentially an XYZ 3D plotter as described by Landers et al [20]. The apparatus has a built-in controller for precise material deposition, and is integrated with PrimCam v2.96 software.…”

Section: Scaffold Fabricationmentioning

confidence: 99%

“…Among these processes, the 3D-plotting technique has shown a great potential in producing reproducible 3D scaffolds featuring interconnected pores and controlled architecture [19]. In contrast to conventional rapid prototyping systems, which are mainly focused on fused deposition, the 3D-plotting technique can be applied to a much larger variety of synthetic and natural materials, including aqueous solutions and pastes [20]. This technique also allows prototyping at body temperature, especially of interest if living cells are incorporated into the plotting material.…”

Section: Introductionmentioning

confidence: 99%

Design and fabrication of 3D‐plotted polymeric scaffolds in functional tissue engineering

Yousefi¹,

Gauvin²,

Sun³

et al. 2007

Polymer Engineering & Sci

View full text Add to dashboard Cite

Regenerating the load‐bearing tissues requires 3D scaffolds that balance the temporary mechanical function with the biological requirements. In functional tissue engineering, designing scaffolds with biomimetic mechanical properties could promote tissue ingrowth since the cells are sensitive to their local mechanical environment. This work aims to design scaffolds that mimic the mechanical response of the biological tissues under physiological loading conditions. Poly(L‐lactide) (PLLA) scaffolds with varying porosities and pore sizes were made by the 3D‐plotting technique. The scaffolds were tested under unconfined ramp compression to compare their stress profile under load with that of bovine cartilage. A comparison between the material parameters estimated for the scaffolds and for the bovine cartilage based on the biphasic theory enabled the definition of an optimum window for the porosity and pore size of these constructs. Moreover, the finite element prediction for the stress distribution inside the scaffolds, surrounded by the host cartilaginous tissue, demonstrated a negligible perturbation of the stress field at the site of implantation. The finite element modeling tools in combination with the developed methodology for optimal porosity/pore size determination can be used to improve the design of biomimetic scaffolds. POLYM. ENG. SCI., 47:608–618, 2007. © 2007 Society of Plastics Engineers.

show abstract

Untitled

Cited by 337 publications

References 8 publications

3D bioprinting of liver-mimetic construct with alginate/cellulose nanocrystal hybrid bioink

3D bioprinting of liver-mimetic construct with alginate/cellulose nanocrystal hybrid bioink

Pancreatic Islet Culture and Preservation Strategies: Advances, Challenges, and Future Outlook

Design and fabrication of 3D‐plotted polymeric scaffolds in functional tissue engineering

Contact Info

Product

Resources

About