Pancreatic Islet Culture and Preservation Strategies: Advances, Challenges, and Future Outlook

Daoud, Jamal; Rosenberg, Lawrence; Tabrizian, Maryam

doi:10.3727/096368910x515872

Cited by 57 publications

(43 citation statements)

References 135 publications

(155 reference statements)

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“…The punctuated pattern of laminin deposition suggests that the process of ECM deposition differs from that of a native islet and is most likely mediated by direct cell-cell contact between βTC3 and MS1 cells during PI formation. The accumulation of ECM proteins may further improve β cell contact and support cell adhesion, both of which can improve β cell function over time [27]. Interestingly, PIs did not include MS1 cells.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Formation of β Cell Pseudoislets Using Islet-Derived Endothelial Cells

et al. 2013

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β cell pseudoislets (PIs) are used for the in vitro study of β-cells in a three-dimensional (3-D) configuration. Current methods of PI induction require unique culture conditions and extensive mechanical manipulations. Here we report a novel co-culture system consisting of high passage β-cells and islet-derived endothelial cells (iECs) that results in a rapid and spontaneous formation of free-floating PIs. PI structures were formed as early as 72 h following co-culture setup and were preserved for more than 14 d. These PIs, composed solely of β-cells, were similar in size to that of native islets and showed an increased percentage of proinsulin-positive cells, increased insulin gene expression in response to glucose stimulation, and restored glucose-stimulated insulin secretion when compared to β-cells cultured as monolayers. Key extracellular matrix proteins that were absent in β-cells cultured alone were deposited by iECs on PIs and were found in and around the PIs. iEC-induced PIs are a readily available tool for examining β cell function in a native 3-D configuration and can be used for examining β-cell/iEC interactions in vitro.

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Section: Discussionmentioning

confidence: 99%

In Vitro Formation of β Cell Pseudoislets Using Islet-Derived Endothelial Cells

et al. 2013

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“…A number of ECM products based on tissues or organs of human origin are already commercially available (Table 1). Since the first use of decellularized bone as a prototype ECM grafting material [503], this fast-growing field has gained convincing proof-of-principle evidence of this approach's efficacy for bone [504], vaginal [505,506], epithelial [507], skin [206,207,209–211], musculoskeletal [508,509], corneal [201,204,510] and vascular [511] tissue repair as well as for tissue engineering of pulmonary [512–514], myocardial [488], airway [515,516], liver [517], renal [518] and pancreatic [519] implants. Most studies reported complete and functional organ regeneration in small-animal models, and early clinical successes with complex tissues in preclinical studies and certain individuals have served as proof of concept [266].…”

Section: Human Tissue Ecm-based Biomaterialsmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

959

513

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Biomaterials have played an increasingly prominent role in the success of biomedical devices and in the development of tissue engineering, which seeks to unlock the regenerative potential innate to human tissues/organs in a state of deterioration and to restore or reestablish normal bodily function. Advances in our understanding of regenerative biomaterials and their roles in new tissue formation can potentially open a new frontier in the fast-growing field of regenerative medicine. Taking inspiration from the role and multi-component construction of native extracellular matrices (ECMs) for cell accommodation, the synthetic biomaterials produced today routinely incorporate biologically active components to define an artificial in vivo milieu with complex and dynamic interactions that foster and regulate stem cells, similar to the events occurring in a natural cellular microenvironment. The range and degree of biomaterial sophistication have also dramatically increased as more knowledge has accumulated through materials science, matrix biology and tissue engineering. However, achieving clinical translation and commercial success requires regenerative biomaterials to be not only efficacious and safe but also cost-effective and convenient for use and production. Utilizing biomaterials of human origin as building blocks for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural tissue with regard to its physical and chemical properties for the orchestration of wound healing and tissue regeneration. In addition to directly using tissue transfers and transplants for repair, new applications of human-derived biomaterials are now focusing on the use of naturally occurring biomacromolecules, decellularized ECM scaffolds and autologous preparations rich in growth factors/non-expanded stem cells to either target acceleration/magnification of the body's own repair capacity or use nature's paradigms to create new tissues for restoration. In particular, there is increasing interest in separating ECMs into simplified functional domains and/or biopolymeric assemblies so that these components/constituents can be discretely exploited and manipulated for the production of bioscaffolds and new biomimetic biomaterials. Here, following an overview of tissue auto-/allo-transplantation, we discuss the recent trends and advances as well as the challenges and future directions in the evolution and application of human-derived biomaterials for reconstructive surgery and tissue engineering. In particular, we focus on an exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications and to provide inspiration for the design of future biomaterials.

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“…As such, it is unclear if identical conditions will work with islets harvested from alternative organisms, such as rat and humans. Notably, islets harvested from different organisms often have different optimal culturing conditions and differing degrees of β-cell proliferation 12,13 .…”

Section: Discussionmentioning

confidence: 99%

Sustained Administration of β-cell Mitogens to Intact Mouse Islets <em>Ex Vivo</em> Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

Pasek

Kavanaugh

Duvall

et al. 2016

JoVE

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The development of biomaterials has significantly increased the potential for targeted drug delivery to a variety of cell and tissue types, including the pancreatic β-cells. In addition, biomaterial particles, hydrogels, and scaffolds also provide a unique opportunity to administer sustained, controllable drug delivery to β-cells in culture and in transplanted tissue models. These technologies allow the study of candidate β-cell proliferation factors using intact islets and a translationally relevant system. Moreover, determining the effectiveness and feasibility of candidate factors for stimulating β-cell proliferation in a culture system is critical before moving forward to in vivo models. Herein, we describe a method to co-culture intact mouse islets with biodegradable compound of interest-loaded poly(lactic-co-glycolic acid) (PLGA) microspheres for the purpose of assessing the effects of sustained in situ release of mitogenic factors on β-cell proliferation. This technique describes in detail how to generate PLGA microspheres containing a desired cargo using commercially available reagents. Additionally, this method utilizes 96-well plates to minimize the amount of reagents necessary to assess β-cell proliferation. This protocol can be readily adapted to use alternative biomaterials and other endocrine cell characteristics such as cell survival and differentiation status.

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Pancreatic Islet Culture and Preservation Strategies: Advances, Challenges, and Future Outlook

Cited by 57 publications

References 135 publications

In Vitro Formation of β Cell Pseudoislets Using Islet-Derived Endothelial Cells

In Vitro Formation of β Cell Pseudoislets Using Islet-Derived Endothelial Cells

Advancing biomaterials of human origin for tissue engineering

Sustained Administration of β-cell Mitogens to Intact Mouse Islets <em>Ex Vivo</em> Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

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Pancreatic Islet Culture and Preservation Strategies: Advances, Challenges, and Future Outlook

Cited by 57 publications

References 135 publications

In Vitro Formation of β Cell Pseudoislets Using Islet-Derived Endothelial Cells

In Vitro Formation of β Cell Pseudoislets Using Islet-Derived Endothelial Cells

Advancing biomaterials of human origin for tissue engineering

Sustained Administration of &#946;-cell Mitogens to Intact Mouse Islets <em>Ex Vivo</em> Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres

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Product

Resources

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Sustained Administration of β-cell Mitogens to Intact Mouse Islets <em>Ex Vivo</em> Using Biodegradable Poly(lactic-co-glycolic acid) Microspheres