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Vallaeys, Tatiana; Albino, Lionel; Soulas, Guy; Wright, Alice Diane; Weightman, Andrew J.

doi:10.1023/a:1005438930870

Cited by 40 publications

(4 citation statements)

References 18 publications

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“…TFD33, Novosphingobium DY4, Pseudomonas sp. NJ10, P. cepacia DBO1, P. aeruginosa NJ15, S. paucimobilis DS-3, and V. paradoxus TV1 . The degradation capacities of these 2,4-D-degrading bacteria have been well documented in previous literature.…”

Section: Discussionmentioning

confidence: 75%

Rapid Biodegradation of the Herbicide 2,4-Dichlorophenoxyacetic Acid by Cupriavidus gilardii T-1

Wang

Liu

et al. 2017

J. Agric. Food Chem.

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Phytotoxicity and environmental pollution of residual herbicides have caused much public concern during the past several decades. An indigenous bacterial strain capable of degrading 2,4-dichlorophenoxyacetic acid (2,4-D), designated T-1, was isolated from soybean field soil and identified as Cupriavidus gilardii. Strain T-1 degraded 2,4-D 3.39 times more rapidly than the model strain Cupriavidus necator JMP134. T-1 could also efficiently degrade 2-methyl-4-chlorophenoxyacetic acid (MCPA), MCPA isooctyl ester, and 2-(2,4-dichlorophenoxy)propionic acid (2,4-DP). Suitable conditions for 2,4-D degradation were pH 7.0-9.0, 37-42 °C, and 4.0 mL of inoculums. Degradation of 2,4-D was concentration-dependent. 2,4-D was degraded to 2,4-dichlorophenol (2,4-DCP) by cleavage of the ether bond and then to 3,5-dichlorocatechol (3,5-DCC) via hydroxylation, followed by ortho-cleavage to cis-2-dichlorodiene lactone (CDL). The metabolites 2,4-DCP or 3,5-DCC at 10 mg L were completely degraded within 16 h. Fast degradation of 2,4-D and its analogues highlights the potential for use of C. gilardii T-1 in bioremediation of phenoxyalkanoic acid herbicides.

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Section: Discussionmentioning

confidence: 75%

Rapid Biodegradation of the Herbicide 2,4-Dichlorophenoxyacetic Acid by Cupriavidus gilardii T-1

Wang

Liu

et al. 2017

J. Agric. Food Chem.

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“…Moreover, identified in a number of plasmids (pEST4011, pIJB1, pAKD25 and pTV1), the two-gene clusters tcbD ORF3 were previously annotated as tfdD [ 61 ], tfdDI [ 36 ], tfdD and orf5 [ 37 ], tfdD and ORF [ 39 ]. The analysis also revealed the presence of a unique two-gene tcbCR gene cluster adjacent to tfd III AKBE of D. acidovorans P4a, previously annotated as tfdRC II [ 59 ].…”

Section: Resultsmentioning

confidence: 99%

“…Among them were both gene clusters annotated as clc and others: clcr1a1b1d1e1 [ 52 ], dccEDCBAR [ 53 ] and tfdFE(tctC)DCS [ 7 ]. Additionally, some clusters were sequenced by different authors, but belonged to the same bacterial strain Pseudomonas knackmussii B13 [ 52 , 61 , 62 , 63 , 64 , 65 , 66 ], Paraburkholderia xenovorans LB400 [ 67 , 68 ], Pseudomonas aeruginosa strain JB2 [ 69 , 70 , 71 ] and Pandoraea pnomenusa MCB032 [ 51 , 72 ]. As a result, the most fully sequenced gene clusters were taken for further analysis based on the latest data deposited in GenBank ( Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

Evolution End Classification of tfd Gene Clusters Mediating Bacterial Degradation of 2,4-Dichlorophenoxyacetic Acid (2,4-D)

Iasakov

2023

IJMS

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The tfd (tfdI and tfdII) are gene clusters originally discovered in plasmid pJP4 which are involved in the bacterial degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) via the ortho-cleavage pathway of chlorinated catechols. They share this activity, with respect to substituted catechols, with clusters tcb and clc. Although great effort has been devoted over nearly forty years to exploring the structural diversity of these clusters, their evolution has been poorly resolved to date, and their classification is clearly obsolete. Employing comparative genomic and phylogenetic approaches has revealed that all tfd clusters can be classified as one of four different types. The following four-type classification and new nomenclature are proposed: tfdI, tfdII, tfdIII and tfdIV(A,B,C). Horizontal gene transfer between Burkholderiales and Sphingomonadales provides phenomenal linkage between tfdI, tfdII, tfdIII and tfdIV type clusters and their mosaic nature. It is hypothesized that the evolution of tfd gene clusters proceeded within first (tcb, clc and tfdI), second (tfdII and tfdIII) and third (tfdIV(A,B,C)) evolutionary lineages, in each of which, the genes were clustered in specific combinations. Their clustering is discussed through the prism of hot spots and driving forces of various models, theories, and hypotheses of cluster and operon formation. Two hypotheses about series of gene deletions and displacements are also proposed to explain the structural variations across members of clusters tfdII and tfdIII, respectively. Taking everything into account, these findings reconstruct the phylogeny of tfd clusters, have delineated their evolutionary trajectories, and allow the contribution of various evolutionary processes to be assessed.

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“…Our data demonstrated that members of the Burkholderiaceae and Comamonadaceae, including Comamonas, Variovorax, and Methylibium, are highly abundant in samples that showed evidence of ametoctradin degradation. These species have been well studied and are commonly associated with xenobiotic degradation (Vallaeys et al, 1998;Nakatsu et al, 2006;Satola et al, 2012;Chen et al, 2016a,b;Öztürk et al, 2020;Zhang et al, 2020). In the packed-bed reactor, Comamonas spp.…”

Section: Discussionmentioning

confidence: 99%

Accelerated Biodegradation of the Agrochemical Ametoctradin by Soil-Derived Microbial Consortia

Whittington

Singh

et al. 2020

Front. Microbiol.

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Pesticide-resistant plant pathogens are an increasing threat to the global food supply and have generated a need for novel, efficacious agrochemicals. The current regulatory process for approving new agrochemicals is a tedious but necessary process. One way to accelerate the safety evaluation process is to utilize in vitro systems to demonstrate pesticide degradation by soil microbes prior to ex vivo soil evaluations. This approach may have the capability to generate metabolic profiles free of inhibitory substances, such as humic acids, commonly present in ex vivo soil systems. In this study, we used a packed-bed microbial bioreactor to assess the role of the natural soil microbial community during biodegradation of the triazolopyrimidine fungicide, ametoctradin. Metabolite profiles produced during in vitro ametoctradin degradation were similar to the metabolite profiles obtained during environmental fate studies and demonstrated the degradation of 81% of the parent compound in 72 h compared to a half-life of 2 weeks when ametoctradin was left in the soil. The microbial communities of four different soil locations and the bioreactor microbiome were compared using high throughput sequencing. It was found that biodegradation of ametoctradin in both ex vivo soils and in vitro in the bioreactor correlated with an increase in the relative abundance of Burkholderiales, well characterized microbial degraders of xenobiotic compounds.

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Cited by 40 publications

References 18 publications

Rapid Biodegradation of the Herbicide 2,4-Dichlorophenoxyacetic Acid by Cupriavidus gilardii T-1

Rapid Biodegradation of the Herbicide 2,4-Dichlorophenoxyacetic Acid by Cupriavidus gilardii T-1

Evolution End Classification of tfd Gene Clusters Mediating Bacterial Degradation of 2,4-Dichlorophenoxyacetic Acid (2,4-D)

Accelerated Biodegradation of the Agrochemical Ametoctradin by Soil-Derived Microbial Consortia

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