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Andrijany, Vinsencia Sri; Indrayanto, Gunawan; Soehono, Loekito Adi

doi:10.1023/a:1006119600153

Cited by 21 publications

(2 citation statements)

References 8 publications

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“…It has been reported that proper concentration microelements have been considered as nutrient factors or as abiotic elicitors, which trigger the formation of secondary metabolites [8,9,16]. Andrijany et al [17] found in callus cultures of Agave amaniensis the relatively high concentration of magnesium, cobalt and copper ions simultaneously inhibited the sapogenin steroid formation while the absence of calcium ions in media induced the increasing in the sapogenin steroid content. It seems to be that the productions of specific useful secondary metabolites by plant cell cultures have intensive researches for economic implications, but until now only a few studies addressing the possibility of date palm steroids production.…”

Section: Discussionmentioning

confidence: 99%

Effect of some Micro-Elements on Steroids Production from Embryogenic Callus of in vitro Date Palm Sakkoty and Bartamuda Cultivars

Sharabasy

2019

By-Products of Palm Trees and Their Applications

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Section: Discussionmentioning

confidence: 99%

Effect of some Micro-Elements on Steroids Production from Embryogenic Callus of in vitro Date Palm Sakkoty and Bartamuda Cultivars

Sharabasy

2019

By-Products of Palm Trees and Their Applications

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“…Somatic embryogenesis was also induced, and several plants were obtained [19][20][21][22]. In addition to micropropagation, there are reports on the production of secondary metabolites by cultures of callus tissue [16,23,24].…”

Section: Introductionmentioning

confidence: 99%

In Vitro-Propagation of Agave tequilana Weber cv. azul in a Temporary Immersion System

V醶quez-Mart韓ez¹,

Nez-Palenius²,

Balch³

et al. 2022

Phyton

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In Mexico, there is a need to produce large quantities of plantlets for the establishment and replanting of blue (cv. azul) agave production areas. Most of these plots are within the origin denomination area (DOT, Spanish acronym) of the distilled product of this plant, known as tequila. The objective of this study was to develop an in vitropropagation protocol for Agave tequilana Weber cv. azul using segmented stems in both: solid and liquid media. A disinfection and in vitro technique were developed to obtain shoots, through plantlets collected in commercial plots, which attained 100% surface-disinfection and budding rate. At the multiplication stage, the effects of 6-Benzylaminopurine (BA) (0.0, 4.4 and 13.2 μM) and kinetin (0.0, 9.4, 18.8 and 37.6 μM) were evaluated on lateralshoot production of segmented sagittal stems. These were cultivated on Murashige & Skoog (MS) medium, with the addition of 3.0% sucrose and 8 g L −1 agar. It was observed that BA and kinetin increased the number of shoots per explant, obtaining up to 18 and 26, respectively. Furthermore, it was found that just the sagittal segmentation of explants increased axillary budding. On the other hand, segmented-stem bases were grown in MS liquid medium with 3.0% sucrose, inside a RITA Ò system, programmed by a 5 min immersion step with a frequency of every 4 h. The effect of Indole−3-Acetic acid (IAA) (0.57, 2.9, 5.7 μM) was evaluated, while maintaining a concentration of BA (13.2 μM). It was observed that the greatest concentration of IAA led to the formation of more than 20 buds per explant. These results offer a new methodology to increase the efficiency of A. tequilana Weber cv. azul-in vitro multiplication by sagittal segmentation of stems and the addition of BA and/or IAA.

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