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Eijlander, R.; Ramanna, M.S.; Jacobsen, E.

doi:10.1023/a:1003041006157

Cited by 8 publications

(2 citation statements)

References 31 publications

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“…A high genetic load carried by a particular genotype may cause sterility, fruitlessness, or lethality on inbreeding. Environmental factors, such as floral age, can cause breakdown in self-incompatibility in young and old flowers (Eijlander et al, 1997;Stephenson et al, 2003). Environmental variation may alter the incompatibility reactions, especially later in the season (East, 1934;Stone et al, 2006;Yasuda, 1934).…”

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Self‐Fertility in a Cultivated Diploid Potato Population Examined with the Infinium 8303 Potato Single‐Nucleotide Polymorphism Array

et al. 2016

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Within a population of F 1 hybrids between two genotypes (S. tuberosum L. Group Phureja DM 1-3 516 R44 [DM] and S. tuberosum L. Group Tuberosum RH89-039-16 [RH]) used in the potato genome sequencing project, we observed fruit set after self-pollination on many plants. Examination of pollen tube growth in self-fertile and self-unfruitful F 1 plants after controlled selfpollinations revealed no difference in the ability of pollen tubes to reach the ovary. To identify genomic regions linked with selffertility, we genotyped the F 1 population using a genome-wide single-nucleotide polymorphism (SNP) array. Polymorphic and robust SNPs were analyzed to identify allelic states segregating with the self-fertile phenotype. All 88 highly significant SNPs occurred on chromosome 12. Seeds obtained after self-pollination of self-fertile individuals were used to advance the population for four generations. Genotyping 46 self-fruitful and 46 self-unfruitful S 3 plants on the Infinium 8303 Potato SNP array revealed eight SNPs segregating with self-fertility on chromosomes 4, 9, 11, and 12. Three times more heterozygosity than expected was found in the S 3 generation. Estimates of heterozygosity were influenced by copy number variation (CNV) in the potato genome leading to spurious heterozygous genotyping calls. Some spurious heterozygosity could be removed by application of a CNV filter developed from alignment of additional monoploid potato genomic sequence to the DM reference genome. The genes responsible for fruit set in self-fertile plants in the F 1 generation were restricted to chromosome 12, whereas new genomic regions contributed to the ability of S 3 plants to set fruit after self-pollination.

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confidence: 99%

Self‐Fertility in a Cultivated Diploid Potato Population Examined with the Infinium 8303 Potato Single‐Nucleotide Polymorphism Array

et al. 2016

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“…DRH lines have shown plasticity in fruit set in previous studies ( Peterson et al., 2016 ; Enciso-Rodriguez et al., 2019 ) and variation may occur due to environmental effects, such as temperature, photoperiod, and humidity as reported in other members of the Solanum genus (such as S. carolinense and S. peruvianum ) or species under the GSI system like citrus ( Webb and Williams, 1988 ; Stone et al., 2006 ; Mena-Ali and Stephenson, 2007 ; Enciso-Rodriguez et al., 2019 ; Montalt et al., 2022 ). Plant age is another possible confounding factor when quantifying SI, as SI breaks down in older plants of S. tuberosum and other Solanum species ( Eijlander et al., 1997 ; Stephenson et al., 2003 ; Travers et al., 2004 ). To account for the effect of plant age, plant fertility was recorded at one month and two and a half months after the plants were transferred from tissue culture.…”

Section: Discussionmentioning

confidence: 99%

HT-B and S-RNase CRISPR-Cas9 double knockouts show enhanced self-fertility in diploid Solanum tuberosum

Lee,

Enciso-Rodriguez,

Behling

et al. 2023

Front. Plant Sci.

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The Gametophytic Self-Incompatibility (GSI) system in diploid potato (Solanum tuberosum L.) poses a substantial barrier in diploid potato breeding by hindering the generation of inbred lines. One solution is gene editing to generate self-compatible diploid potatoes which will allow for the generation of elite inbred lines with fixed favorable alleles and heterotic potential. The S-RNase and HT genes have been shown previously to contribute to GSI in the Solanaceae family and self-compatible S. tuberosum lines have been generated by knocking out S-RNase gene with CRISPR-Cas9 gene editing. This study employed CRISPR-Cas9 to knockout HT-B either individually or in concert with S-RNase in the diploid self-incompatible S. tuberosum clone DRH-195. Using mature seed formation from self-pollinated fruit as the defining characteristic of self-compatibility, HT-B-only knockouts produced little or no seed. In contrast, double knockout lines of HT-B and S-RNase displayed levels of seed production that were up to three times higher than observed in the S-RNase-only knockout, indicating a synergistic effect between HT-B and S-RNase in self-compatibility in diploid potato. This contrasts with compatible cross-pollinations, where S-RNase and HT-B did not have a significant effect on seed set. Contradictory to the traditional GSI model, self-incompatible lines displayed pollen tube growth reaching the ovary, yet ovules failed to develop into seeds indicating a potential late-acting self-incompatibility in DRH-195. Germplasm generated from this study will serve as a valuable resource for diploid potato breeding.

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A Structural Investigation of Trivalent and Divalent Rare Earth Thiocyanate Complexes Synthesised by Redox Transmetallation

et al. 2010

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Cited by 8 publications

References 31 publications

Self‐Fertility in a Cultivated Diploid Potato Population Examined with the Infinium 8303 Potato Single‐Nucleotide Polymorphism Array

Self‐Fertility in a Cultivated Diploid Potato Population Examined with the Infinium 8303 Potato Single‐Nucleotide Polymorphism Array

HT-B and S-RNase CRISPR-Cas9 double knockouts show enhanced self-fertility in diploid Solanum tuberosum

A Structural Investigation of Trivalent and Divalent Rare Earth Thiocyanate Complexes Synthesised by Redox Transmetallation

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