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Gijzen, Mark; Miller, S. Shea; Bowman, Lu-Ann; Batchelor, Anthea K.; Boutilier, Kim; Miki, Brian

doi:10.1023/a:1006244500951

Cited by 19 publications

References 13 publications

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Discrimination of Pinus eldarica Medw. and its two new species by epicuticular wax, lignin content, electrophoretic isozyme and activity of peroxidase

et al. 2008

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Mondell pine (Pinus eldarica Medw.) is a rare and drought resistant pine, found naturally only in semi desert environment southeast of Tbilisi, Georgia, and has been probably introduced to Iran more than 800 years ago. This species has gradually altered both in shape and growth rate in Nashtifan‐Khaf, and generated two species nova which so called as ball shaped and conical, according to characteristics of their shape and smaller stature. This study was conducted to discriminate these species nova using epicuticular wax features and certain biochemical characteristics i.e., peroxidase electrophoretic pattern (PAGE), the activity of soluble (SPO), ionically (IPO) and covalently (CPO) wall‐bound fractions of peroxidase, lignin and total protein content. Observation via scanning electron microspopy showed differences that in adaxial and abaxial surface of needles, and also seed surface, in ball shaped type with the others. But none of the biochemical characteristics had significant difference in these three types. The electrophoretic analysis showed that the peroxidase isozyme is not suitable marker for discriminating the species nova from wild type, in spite of obvious micro‐ and macromorphological differences. Results of current study, propose the use of molecular markers and cytogenetic studies in order to specify cause of species nova occurrence in the study area. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Discrimination of Pinus eldarica Medw. and its two new species by epicuticular wax, lignin content, electrophoretic isozyme and activity of peroxidase

et al. 2008

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The seed coat-specific expression of a subtilisin-like gene, SCS1 , from soybean

Batchelor

Boutilier

Miller

et al. 2000

Planta

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A seed coat-specific gene, SCS1 (Seed Coat Subtilisin 1), from soybean, Glycine max [L.] Merill, has been identified and studied. The gene belongs to a small family of genes with sequence similarity to the subtilisins, which are serine proteases. Northern blot analysis showed that SCS1 RNA accumulates to maximal levels in seed coats at 12 days post anthesis, preceding the final stages of seed coat differentiation. The SCS1 RNA was not found in other tissues including embryos, seed pods, flowers, stems, roots or leaves. In-situ hybridization studies confirmed the temporal pattern of expression observed by Northern blot analysis and further revealed a restricted pattern of RNA accumulation in thick-walled parenchyma cells of the seed coats. These cells are important in the apoplastic translocation of nutrients en route to the embryo from the vascular tissues. The tissue-specific subtilisin-like gene may be required for regulating the differentiation of the thick-walled parenchyma cells.

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Biochemical Responses of Chestnut Oak to a Galling Cynipid

Allison

Schultz

2005

J Chem Ecol

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Abstract-We characterized the distribution of nutritional and defensive biochemical traits in galls elicited on chestnut oak (Quercus prinus L.) by the gall wasp Andricus petiolicolus Basse (Cynipidae) in comparison with gypsy moth-wounded and unwounded leaves. Gall cortex and epidermis exhibited elevated soluble peroxidase (POX) and soluble invertase activities, and greater condensed tannin concentrations than did nutritive tissues or leaves. Nutritive tissue, on which the insect feeds, contained few polyphenols, and lower POX and invertase activities compared with other gall tissues and leaves. Elevated total POX activity arose from a complex pattern of enhanced and suppressed isoform activities in galls. Invertase enzyme activity decreased in all tissues over the course of the 7-d study, although gypsy moth wounding suppressed this decline slightly in ungalled leaves. Our results indicate that the distribution of biochemical defenses in this typical cynipid gall differs significantly from the leaf tissue from which it is formed and support a role for invertases in establishing the gall as a sink. A. petiolicolus larvae do not induce, and may suppress, plant defense responses in nutritive tissue, while enzymatic activity and phenolic accumulation are enhanced in gall tissues surrounding feeding sites. These patterns suggest that the gall is manipulated by the insect to enhance its food and protective value.

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Cited by 19 publications

References 13 publications

Discrimination of Pinus eldarica Medw. and its two new species by epicuticular wax, lignin content, electrophoretic isozyme and activity of peroxidase

Discrimination of Pinus eldarica Medw. and its two new species by epicuticular wax, lignin content, electrophoretic isozyme and activity of peroxidase

The seed coat-specific expression of a subtilisin-like gene, SCS1 , from soybean

Biochemical Responses of Chestnut Oak to a Galling Cynipid

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