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Тараскина, А. Е.; Savicheva, Alevtina M.; Akopian, T. A.; Soroka, Aliza; Momynaliev, K. Т.; Govorun, Vadim M.

doi:10.1023/a:1020664807029

Cited by 7 publications

(3 citation statements)

References 6 publications

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“…The development of tetracycline resistance in mycoplasmas in some cases is associated with the acquisition of tet(M) determinants located at the Tn916 transposon [ 39 ]. The transposon encodes the TetM protein, protecting ribosomes from the effects of tetracyclines.…”

Section: Discussionmentioning

confidence: 99%

Mycoplasmas and Their Antibiotic Resistance: The Problems and Prospects in Controlling Infections

et al. 2016

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The present review discusses the problem of controlling mycoplasmas (class Mollicutes), the smallest of self-replicating prokaryotes, parasites of higher eukaryotes, and main contaminants of cell cultures and vaccines. Possible mechanisms for the rapid development of resistance to antimicrobial drugs in mycoplasmas have been analyzed. Omics technologies provide new opportunities for investigating the molecular basis of bacterial adaptation to stress factors and identifying resistomes, the total of all genes and their products contributing to antibiotic resistance in microbes. The data obtained using an integrated approach with post-genomics methods show that antibiotic resistance may be caused by more complex processes than has been believed heretofore. The development of antibiotic resistance in mycoplasmas is associated with essential changes in the genome, proteome, and secretome profiles, which involve many genes and proteins related to fundamental cellular processes and virulence.

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Section: Discussionmentioning

confidence: 99%

Mycoplasmas and Their Antibiotic Resistance: The Problems and Prospects in Controlling Infections

et al. 2016

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“…A large percentage of microorganisms containing the tetM gene provide conditions for the transfer of the determinant to mycoplasma cells. Dystrophic changes in the urogenital mucosa and desquamation of the epithelium also facilitate the adhesion of microorganisms, mycoplasmas in particular (34).…”

Section: Discussionmentioning

confidence: 99%

Molecular diagnostic of Ureaplasma urealyticum presence and tetracycline resistance in urine samples

Vică

Matei

Katona

et al. 2021

Revista Romana De Medicina De Laborator

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Sexually transmitted infections (STIs) are among the most common infections in Romania. Infection with Ureaplasma urelyticum is one of the major causes of STIs and can cause serious complications. Although tetracycline is the drug commonly used to treat infections caused by U. urealyticum, several studies indicate the emergence and rapid development of strains resistant to these antibiotics in the United States or Europe. Tetracycline resistance in bacteria is encoded by a number of different genetic determinants but in mycoplasmas the only tetracycline resistance determinant that has been reported is the tetM gene. Tetracycline resistance among Ureaplasma spp. is associated with the presence of the horizontally acquired tetM resistance gene. Our study on bacterial DNA aimed to determine the presence of tetracycline-resistant U. urealyticum strains, by identifying the presence of the tetM gene. We used first void urine samples from 622 STI-suspected subjects. DNA was extracted, purified and amplified via PCR for the simultaneous detection of 6 STIs. 68 patients were diagnosed with U. urealyticum. DNA obtained from these samples was amplified using the tetM gene and U. urealyticum - specific urease gene primers. The urease gene was amplified in all samples, confirming the presence of U. urealyticum. The tetM gene was amplified in 2 samples considered tetracycline-resistant strains. The study confirmed the presence of U. urealyticum strains resistant to tetracycline in Romania. The employed technique can produce quick results both for U. urealyticum detection and determination of its resistance to tetracycline using a single easy-to-collect biological sample.

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“…The tetM gene for this genome is accepted to be part of a Tn916 a conjugative transposon also called integrative conjugative element (ICE). Tetracycline resistance for many U. parvum and U. urealyticum genomes have been reported to be due to transposon-associated tetM gene presence in the bacterial genome from isolates from distant countries (Beeton et al , 2009; de Barbeyrac et al , 1996; Govender et al , 2012; Mardassi et al , 2012; Roberts 1990; Taraskina et al , 2002) and donation from transposon-carrying Enterococcus faecalis to a close relative to Ureaplasma ( Mycoplasma hominis ) during co-culture was demonstrated through mating at a frequency of 10 −6 to 10 −7 (Roberts and Kenny 1987). …”

Section: Introductionmentioning

confidence: 99%

Random insertion and gene disruption via transposon mutagenesis of Ureaplasma parvum using a mini-transposon plasmid

Aboklaish

Dordet‐Frisoni

Citti

et al. 2014

International Journal of Medical Microbiology

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While transposon mutagenesis has been successfully used for Mycoplasma spp. to disrupt and determine non-essential genes, previous attempts with Ureaplasma spp. have been unsuccessful. Using a polyethylene glycol-transformation enhancing protocol, we were able to transform three separate serovars of Ureaplasma parvum with a Tn4001-based mini-transposon plasmid containing a gentamicin resistance selection marker. Despite the large degree of homology between Ureaplasma parvum and Ureaplasma urealyticum, all attempts to transform the latter in parallel failed, with the exception of a single clinical U. urealyticum isolate. PCR probing and sequencing were used to confirm transposon insertion into the bacterial genome and identify disrupted genes. Transformation of prototype serovar 3 consistently resulted in transfer only of sequence between the mini-transposon inverted repeats, but some strains showed additional sequence transfer. Transposon insertion occurred randomly in the genome resulting in unique disruption of genes UU047, UU390, UU440, UU450, UU520, UU526, UU582 for single clones from a panel of screened clones. An intergenic insertion between genes UU187 and UU188 was also characterised. Two phenotypic alterations were observed in the mutated strains: Disruption of a DEAD-box RNA helicase (UU582) altered growth kinetics, while the U. urealyticum strain lost resistance to serum attack coincident with disruption of gene UUR10_137 and loss of expression of a 41 kDa protein. Transposon mutagenesis was used successfully to insert single copies of a mini-transposon into the genome and disrupt genes leading to phenotypic changes in Ureaplasma parvum strains. This method can now be used to deliver exogenous genes for expression and determine essential genes for Ureaplasma parvum replication in culture and experimental models.

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Cited by 7 publications

References 6 publications

Mycoplasmas and Their Antibiotic Resistance: The Problems and Prospects in Controlling Infections

Mycoplasmas and Their Antibiotic Resistance: The Problems and Prospects in Controlling Infections

Molecular diagnostic of Ureaplasma urealyticum presence and tetracycline resistance in urine samples

Random insertion and gene disruption via transposon mutagenesis of Ureaplasma parvum using a mini-transposon plasmid

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