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Пирузян, Е. С.; Богуш, В. Г.; Sidoruk, K. V.; Goldenkova, I. V.; Mysiychuk, K. A.; Дебабов, В. Г.

doi:10.1023/a:1025187311024

Cited by 23 publications

(3 citation statements)

References 22 publications

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“…Plants like tobacco, potato and Arabidopsis thaliana have further been employed as transgenic expression hosts for synthetic silk genes, grown in both greenhouse and field trials ( Table 3). [30][31][32][47][48][49] Therein, the silk proteins have been targeted to different compartments in order to gain high yields. In tobacco leaves a yield of 2% (w/w) of total protein was reached when targeted to the endoplasmic reticulum.…”

Section: Production Of Engineered Silk Proteinsmentioning

confidence: 99%

Biotechnological Production of Spider‐Silk Proteins Enables New Applications

Vendrely

Scheibel

2007

Macromolecular Bioscience

220

212

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The outstanding mechanical properties of spider silks have motivated many researchers to establish biotechnological production techniques which are necessary to provide sufficient amounts of silk proteins for industrial applications. Based on recent developments in genetic engineering, two strategies for the recombinant production of spider-silk proteins have been established which are discussed in detail. Further, protein-design strategies are described, enabling the combination of silk properties with additional biological, chemical, or technical features. We highlight the potential of engineered and recombinantly-produced spider-silk proteins to provide the basis for a new generation of biomaterials.

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Section: Production Of Engineered Silk Proteinsmentioning

confidence: 99%

Biotechnological Production of Spider‐Silk Proteins Enables New Applications

Vendrely

Scheibel

2007

Macromolecular Bioscience

220

212

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“…The disadvantage of prokaryotic expression systems is their inherent limitation in the size of synthetic genes. Another approach employs eukaryotic hosts, such as yeast, Bombyx mori , transgenic plants, bovine mammary epithelial alveolar cells, etc., which produce recombinant proteins most closely resembling natural silks [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. Unlike the above-mentioned genetic engineering methods, chemical synthesis only replaces some regions of the silk protein with organic polymers [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of Spider Silk Protein Structure on Mechanical and Biological Properties for Energetic Material Detection

Peng,

Liu,

Gao

et al. 2024

Molecules

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Spider silk protein, renowned for its excellent mechanical properties, biodegradability, chemical stability, and low immune and inflammatory response activation, consists of a core domain with a repeat sequence and non-repeating sequences at the N-terminal and C-terminal. In this review, we focus on the relationship between the silk structure and its mechanical properties, exploring the potential applications of spider silk materials in the detection of energetic materials.

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“…Tobacco and potatoes were used for spidroin production starting in 2003; however, these materials are not widely used. , The recombinant protein yield typically does not exceed 0.5% of total plant protein. The maximal reported yield was 400 mg of protein from 6 kg of tobacco leaves .…”

Section: Introductionmentioning

confidence: 99%

Recombinant Spidroins as the Basis for New Materials

Дебабов

Богуш

2020

ACS Biomater. Sci. Eng.

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Spider web proteins are unique materials created by nature that, considering the combination of their properties, do not have analogues among natural or human-created materials. Obtaining significant amounts of these proteins from natural sources is not feasible. Biotechnological manufacturing in heterological systems is complicated by the very high molecular weight of spidroins and their specific amino acid composition. Obtaining recombinant analogues of spidroins in heterological systems, mainly in bacteria and yeast, has become a compromise solution. Because they can self-assemble, these proteins can form various materials, such as fibers, films, 3D-foams, hydrogels, tubes, and microcapsules. The effectiveness of spidroin hydrogels in deep wound healing, as 3D scaffolds for bone tissue regeneration and as oriented fibers for axon growth and nerve tissue regeneration, was demonstrated in animal models. The possibility to use spidroin micro- and nanoparticles for drug delivery was demonstrated, including the use of modified spidroins for virus-free DNA delivery into animal cell nuclei. In the past few years, significant interest has arisen concerning the use of these materials as biocompatible and biodegradable soft optics to construct photonic crystal super lenses and fiber optics and as soft electronics to use in triboelectric nanogenerators. This review summarizes the latest achievements in the field of spidroin production, the creation of materials based on them, the study of these materials as a scaffold for the growth, proliferation, and differentiation of various types of cells, and the prospects for using these materials for medical applications (e.g., tissue engineering, drug delivery, coating medical devices), soft optics, and electronics. Accumulated data suggest the use of recombinant spidroins in medical practice in the near future.

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Untitled

Cited by 23 publications

References 22 publications

Biotechnological Production of Spider‐Silk Proteins Enables New Applications

Biotechnological Production of Spider‐Silk Proteins Enables New Applications

Influence of Spider Silk Protein Structure on Mechanical and Biological Properties for Energetic Material Detection

Recombinant Spidroins as the Basis for New Materials

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