Biotechnological Production of Spider‐Silk Proteins Enables New Applications

Vendrely, Charlotte; Scheibel, Thomas

doi:10.1002/mabi.200600255

Cited by 220 publications

(224 citation statements)

References 78 publications

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“…In addition, venoms of spiders have been evaluated for the treatment of cardiac arrhythmia (Novak 2001), Alzheimer's disease (Lewis and Garcia 2003) and erectile dysfunction (Andrade et al 2008). Owing to the combination of excellent mechanical properties, biocompatibility, and slow biodegradability, spider silk has found tremendous applications in biomedical field, such as tissue engineering (Vendrely and Scheibel 2007). As additives in cosmetic products, such as shampoos, soaps, creams, and nail varnish, they enhance the brightness, softness, and/or toughness of the products (Vendrely and Scheibel 2007).…”

Section: Spider Cobwebmentioning

confidence: 99%

“…Owing to the combination of excellent mechanical properties, biocompatibility, and slow biodegradability, spider silk has found tremendous applications in biomedical field, such as tissue engineering (Vendrely and Scheibel 2007). As additives in cosmetic products, such as shampoos, soaps, creams, and nail varnish, they enhance the brightness, softness, and/or toughness of the products (Vendrely and Scheibel 2007). Furthermore, spider-silk fibers could be applied in technical textiles (used for example in parachutes and bullet-proof vests) which demand high toughness in combination with sleaziness (Vendrely and Scheibel 2007).…”

Section: Spider Cobwebmentioning

confidence: 99%

“…As additives in cosmetic products, such as shampoos, soaps, creams, and nail varnish, they enhance the brightness, softness, and/or toughness of the products (Vendrely and Scheibel 2007). Furthermore, spider-silk fibers could be applied in technical textiles (used for example in parachutes and bullet-proof vests) which demand high toughness in combination with sleaziness (Vendrely and Scheibel 2007). The production of spider silk in bacteria has been achieved through genetic engineering.…”

Section: Spider Cobwebmentioning

confidence: 99%

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Cobweb as novel biomaterial for the green and eco-friendly synthesis of silver nanoparticles

et al. 2015

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In this study, spider cobweb as a novel biomaterial was used for the green synthesis of silver nanoparticles (AgNPs). The synthesized AgNPs were characterized using UV-Vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy. The efficacy of biosynthesized particles as antibacterial agents was evaluated using multidrug resistant clinical bacterial isolates through sensitivity testing with AgNPs and combination of AgNPs with some selected antibiotics. In addition, the potential application of the particles as additives in paints was demonstrated using some bacterial and fungal isolates. The synthesized AgNPs which were dark brown in color displayed maximum absorbance at the wavelength of 436 nm. It was observed that the reaction mixture of 1:40 (extract:AgNO 3 solution) at pH of 8.5 produced particles with maximum absorbance at 436 nm. The FTIR spectrum showed peaks at 3298, 2359, 2089, and 1635 cm -1 , indicating that proteins were the capping and stabilization molecules in the synthesis of AgNPs. The particles were spherical in shape with size ranging about 3-50 nm. The energy-dispersive X-ray analysis showed the presence of silver as the most prominent metal, while the selected area electron diffraction pattern conformed to the face-centered cubic phase and crystalline nature of AgNPs. The AgNPs inhibited the growth of several bacterial isolates including S. aureus, E. coli, Klebsiella granulomatis and P. aeruginosa in the range of 10-17 mm at concentration of 100 lg/ml. It was also demonstrated that AgNPs potentiated the activities of augmentin, ofloxacin and cefixime in the AgNP-antibiotic synergy studies. Similarly, the inclusion of AgNPs as additive in white emulsion paint led to the total inhibition of growth of E. coli, P. aeruginosa, Aspergillus niger and A. fumigatus. To the best of our knowledge, this is the first report of the use of cobweb for the green synthesis of AgNPs. The immense antimicrobial activities of the particles can be explored in the creation of novel products, where it can be used as additive to protect materials against microbial attack.

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Section: Spider Cobwebmentioning

confidence: 99%

Section: Spider Cobwebmentioning

confidence: 99%

Section: Spider Cobwebmentioning

confidence: 99%

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Cobweb as novel biomaterial for the green and eco-friendly synthesis of silver nanoparticles

et al. 2015

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“…The preliminary thread is pulled through the spinning wart using, for example, the hind legs. (Vendrely & Scheibel 2007;SenGupta & Scheibel 2008). Some of these problems have been solved by expressing authentic spider cDNA in eukaryotic expression systems such as yeast, or transgenetic plants such as potato or tobacco (Scheller et al 2001).…”

Section: Molecular Biomimetics Of Proteins: Four Case Studiesmentioning

confidence: 99%

Mimicking biopolymers on a molecular scale: nano(bio)technology based on engineered proteins

Grunwald

Rischka

Kast

et al. 2009

Phil. Trans. R. Soc. A.

Self Cite

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Proteins are ubiquitous biopolymers that adopt distinct three-dimensional structures and fulfil a multitude of elementary functions in organisms. Recent systematic studies in molecular biology and biotechnology have improved the understanding of basic functional and architectural principles of proteins, making them attractive candidates as concept generators for technological development in material science, particularly in biomedicine and nano(bio)technology. This paper highlights the potential of molecular biomimetics in mimicking high-performance proteins and provides concepts for applications in four case studies, i.e. spider silk, antifreeze proteins, blue mussel adhesive proteins and viral ion channels.

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“…Although the chemical composition of silk is thought to vary to match the functional requirement of each species, it largely consists of two main structural proteins, fibroin and sericin. [29][30][31] There is much ongoing research into the effects of various combinations of silk source, processing techniques, structure, and topography, with each combination significantly altering the silk properties and its biocompatibility. 26 However, it is generally believed that the glue-like sericin component is immunogenic, and once the silk has been degummed to remove the sericin, the remaining silk fibroin is highly biocompatible.…”

Section: Introductionmentioning

confidence: 99%

In VitroEvaluation of a Novel Non-Mulberry Silk Scaffold for Use in Tendon Regeneration

Musson

Naot

Chhana

et al. 2015

Tissue Engineering Part A

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Tearing of the rotator cuff tendon in the shoulder is a significant clinical problem, with large/full-thickness tears present in *22% of the general population and recurrent tear rates postarthroscopic repair being quoted as high as 94%. Tissue-engineered biomaterials are increasingly being investigated as a means to augment rotator cuff repairs, with the aim of inducing host cell responses to increase tendon tissue regeneration. Silk-derived materials are of particular interest due to the high availability, mechanical strength, and biocompatibility of silks. In this study, Spidrex Ò , a novel knitted, non-mulberry silk fibroin scaffold was evaluated in vitro for its potential to improve tendon regeneration. Spidrex was compared with a knitted Bombyx mori silk scaffold, a 3D collagen gel and Fiberwire Ò suture material. Primary human and rat tenocytes successfully adhered to Spidrex and significantly increased in number over a 14 day period ( p < 0.05), as demonstrated by fluorescent calcein-AM staining and alamarBlue Ò assays. A similar growth pattern was observed with human tenocytes cultured on the B. mori scaffold. Morphologically, human tenocytes elongated along the silk fibers of Spidrex, assuming a tenocytic cell shape, and were less circular with a higher aspect ratio compared with human tenocytes cultured on the B. mori silk scaffold and within the collagen gel ( p < 0.05). Gene expression analysis by real-time PCR showed that rat tenocytes cultured on Spidrex had increased expression of tenocyte-related genes such as fibromodullin, scleraxis, and tenomodulin ( p < 0.05). Expression of genes that indicate transdifferentiation toward a chondrocytic or osteoblastic lineage were significantly lower in tenocytes cultured on Spidrex in comparison to the collagen gel ( p < 0.05). Immunogenicity assessment by the maturation of and cytokine release from primary human dendritic cells demonstrated that Spidrex enhanced dendritic cell maturation in a similar manner to the clinically used suture material Fiberwire, and significantly upregulated the release of proinflammatory cytokines ( p < 0.05). This suggests that Spidrex may induce an early immune response postimplantation. While further work is required to determine what effect this immune response has on the tendon healing process, our in vitro data suggests that Spidrex may have the cytocompatibility and bioactivity required to support tendon regeneration in vivo.

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Biotechnological Production of Spider‐Silk Proteins Enables New Applications

Cited by 220 publications

References 78 publications

Cobweb as novel biomaterial for the green and eco-friendly synthesis of silver nanoparticles

Cobweb as novel biomaterial for the green and eco-friendly synthesis of silver nanoparticles

Mimicking biopolymers on a molecular scale: nano(bio)technology based on engineered proteins

In VitroEvaluation of a Novel Non-Mulberry Silk Scaffold for Use in Tendon Regeneration

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