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Sreekumar, S.; Seeni, S.; Pushpangadan, P.

doi:10.1023/a:1006486817203

Cited by 68 publications

(7 citation statements)

References 26 publications

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“…The maximum root induction (70%), root length (6.7 cm), and number of roots (8.76 roots per shoot) were observed when MS medium was fortified with IBA (1.2 mg L -1 ) and IAA (0.5 mg L -1 ) (Table 3 & Figure 1D). The same results have already been confirmed by many in vitro studies (Sreekumar et al, 2000;Ramulu et al, 2002;Martin, 2002;Lemma et al, 2020;Prajapati et al, 2023;Sarropoulou et al, 2023). The in vitro rooted plantlets were successfully transferred to plastic cups filled with a hardening medium containing garden soil, sand, and vermicompost (1:1:1) and maintained in the greenhouse (Figure 1e).…”

Section: Discussionsupporting

confidence: 78%

In vitro regeneration of plantlets from leaf segments of Pupalia lappacea (L.) Juss. for mass propagation

Vijayan,

Vijayakumar

2023

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The in vitro study was carried out to standardize the protocol for induction of callus, proliferation of shoots, initiation of roots, and plant regeneration in Pupalia lappacea (L.) Juss. using the artificial nutrient culture medium. Maximum callus induction (76%) from the young leaf explants was achieved by using MS medium supplemented with 0.5 mg L-1 of 2, 4-D and 2.5 mg L-1 of NAA. The maximum shoot induction (78%) was observed on MS medium containing 2.0 mg/L of BAP and 0.8 mg/L of GA3. The highest root initiation (70%) was obtained on MS medium with IBA (1.2 mg L-1) and IAA (0.5 mg L-1). The acclimation process was successfully completed in the garden soil with a better survival rate (72%).

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Section: Discussionsupporting

confidence: 78%

In vitro regeneration of plantlets from leaf segments of Pupalia lappacea (L.) Juss. for mass propagation

Vijayan,

Vijayakumar

2023

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“…Similar to our results, Mestinšek-Mubi et al [ 34 ] showed that basal-derived nodal explants resulted in maximum shoot length and node numbers, which were significantly greater than apical-derived explants in two drug-type cannabis genotypes (MX-CBD-11 and MX-CBD-707). Comparable results were also reported with other plant species such as Salvia tomentosa [ 21 ], Rauvolfia serpentina [ 35 ], Quercus robur [ 36 ], Persea americana [ 37 ], Solanum tuberosum [ 38 ], Ginkgo biloba [ 20 ], Rubus fruticosus [ 39 ], Caralluma lasiantha [ 40 ], Psidium guajava [ 41 ], Hemidesmus indicus [ 42 ], Ficus religiosa [ 43 ], and Cedrela montana [ 44 ] which showed that explants derived from basal parts had better performance than those apical-derived explants. The endogenous balance of phytohormones in the explants obtained from the basal and near-basal parts of the mother plant contributes to the emergence of their juvenile characteristics [ 35 , 45 ].…”

Section: Discussionsupporting

confidence: 63%

Effect of Explant Source on Phenotypic Changes of In Vitro Grown Cannabis Plantlets over Multiple Subcultures

Hesami

Adamek

Pepe

et al. 2023

Biology

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Drug-type cannabis is often multiplied using micropropagation methods to produce genetically uniform and disease/insect-free crops. However, micropropagated plantlets often exhibit phenotypic variation, leading to culture decline over time. In cannabis, the source of these changes remains unknown, though several factors (e.g., explant’s sources and prolonged in vitro culture) can result in such phenotypical variations. The study presented herein evaluates the effects of explant sources (i.e., nodal segments derived from the basal, near-basal, middle, and apical parts of the greenhouse-grown mother plant) over multiple subcultures (4 subcultures during 235 days) on multiplication parameters and leaf morphological traits of in vitro cannabis plantlets. While initial in vitro responses were similar among explants sourced from different regions of the plant, there were significant differences in performance over the course of multiple subcultures. Specifically, explant source and/or the number of subcultures significantly impacted plantlet height, number of nodes, and canopy surface area. The explants derived from the basal and near-basal parts of the plant resulted in the tallest shoots with the greatest number of nodes, while the explants derived from the middle and apical regions led to shorter shoots with fewer nodes. Moreover, the basal-derived explants produced cannabis plantlets with shorter but wider leaves which demonstrated the potential of such explants for in vitro rejuvenation practices with minimal culture decline. This study provides new evidence into the long-term impacts of explant source in cannabis micropropagation.

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“…The efficiency of half strength MS PGR-free medium and/ or IBA in rooting has been reported for many Asclepiadaceae plants (Komalavalli and Rao 2000;Sreekumar et al 2000;Martin 2002;Beena et al 2003). Patnaik and Debata (1996) further observed that IBA was more effective than IAA for the induction of rooting in Hemidesmus indicus.…”

Section: Rooting and Acclimatizationmentioning

confidence: 79%

In vitro propagation of Huernia hystrix: an endangered medicinal and ornamental succulent

Amoo

Finnie

Staden

2009

Plant Cell Tiss Organ Cult

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An efficient and rapid method for in vitro clonal propagation of Huernia hystrix was developed, resulting in shoot regeneration within 3 weeks of culture. This endangered medicinal and ornamental succulent is in high demand. Multiple shoots were regenerated from stem explants (10 mm length) cultured on Murashige and Skoog (MS) medium containing 3% sucrose and supplemented with a range of NAA (0.00-8.06 lM) and BA (4.44-22.19 lM) concentrations. A 100% shoot response with a multiplication rate of four shoots per explant was obtained on MS medium containing 5.37 lM NAA and 22.19 lM BA. Callus produced at the base of the explant on the same medium showed root organogenic potential. The in vitro regenerated shoots produced roots when transferred to half strength MS medium with or without auxin. The micropropagated plants were easily acclimatized within 2 months under greenhouse conditions when potted in a soil and sand mixture (1:1; v/v) treated with a fungicide (Benlate, 0.01%). More than 95% survival with no observable morphological variations was obtained. The developed protocol provides a simple, cost-effective means for the conservation of endangered H. hystrix by clonal propagation within a short time.

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Cited by 68 publications

References 26 publications

In vitro regeneration of plantlets from leaf segments of Pupalia lappacea (L.) Juss. for mass propagation

In vitro regeneration of plantlets from leaf segments of Pupalia lappacea (L.) Juss. for mass propagation

Effect of Explant Source on Phenotypic Changes of In Vitro Grown Cannabis Plantlets over Multiple Subcultures

In vitro propagation of Huernia hystrix: an endangered medicinal and ornamental succulent

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