Along with the sequent completion of Manwan and Dachaoshan Dam, the river continuum of the middle and lower reaches of the Lancang River was separated into three types of segments: reservoir, below-dam segment and downstream flowing segment. The long-term series of water quality and river flow data over 20 years were analyzed in order to study the impact of dam construction and operation on water quality and water self-purification capacity of these different river segments. From pre-dam period to the first 7 years after Manwan Dam had been accomplished, the water quality of Manwan Reservoir became worse due to the accumulation of pollutants, and then to the next 5 years the water quality became better in virtue of the water self-purification of the reservoir. The cooperative operation of Manwan and Dachaoshan Dam had cumulatively positive impacts on water quality of their below-dam segment but no impacts on that of downstream flowing segment. From pre-dam period to the first 7 years after the closure of Manwan Dam, the water selfpurification capacity of Xiaowan-Manwan segment for BOD 5 , COD Mn and NH 3 -N decreased. Also, the water self-purification capacity of Manwan-Dachaoshan segment for BOD 5 and COD Mn decreased but for NH 3 -N increased. However, the water self-purification capacity of Jinghong-Ganlanba segment changed contrary to Manwan-Dachaoshan segment. In general, the construction of Manwan Dam negatively affected the water self-purification capacity of reservoir and below-dam segment but impose little impact on that of downstream flowing segment. This study suggested that it is necessary to pay attention to the effect of complicated temporal and spatial characteristics of dam on aquatic ecosystem.