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Пономаренко, Н. А.; Aleksandrova, E. S.; Vorobiev, I. I.; Durova, Oxana M.; Kozyr, A. V.; Kolesnikov, Alexander V.; Telegin, G. B.; Kalinina, A. R.; Suchkov, S V; Gabibov, Alexander

doi:10.1023/a:1026619805983

Cited by 5 publications

(1 citation statement)

References 2 publications

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“…UV spectrophotometry also demonstrated that anti-poly-(dT) specific ssDNA antibody purified from normal serum did not hydrolyze the probe (Figures 4(a) and 4(b)). This preliminary analysis was performed on four lupus patient serum samples (LP 1–4) and four normal donor serum samples (ND 1–4) and produced results consistent with results of analysis by Agilent and of previous studies which have demonstrated that lupus anti-DNA antibodies hydrolyze DNA while normal anti-DNA antibodies do not [6, 15, 17, 20]. …”

Section: Resultssupporting

confidence: 76%

A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus

Cavallo

Kats

Chen

et al. 2012

Autoimmune Diseases

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Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by the production of antibodies against a variety of self-antigens including nucleic acids. These antibodies are cytotoxic, catalytic (hydrolyzing DNA, RNA, and protein), and nephritogenic. Current methods for investigating catalytic activities of natural abzymes produced by individuals suffering from autoimmunity are mostly discontinuous and often employ hazardous reagents. Here we demonstrate the utility of dual-labeled, fluorogenic DNA hydrolysis probes in highly specific, sensitive, continuous, fluorescence-based measurement of DNA hydrolytic activity of anti-ssDNA abzymes purified from the serum of patients suffering from SLE. An assay for the presence and levels of antibodies exhibiting hydrolytic activity could facilitate disease diagnosis, prediction of flares, monitoring of disease state, and response to therapy. The assay may allow indirect identification of additional targets of anti-DNA antibodies and the discovery of molecules that inhibit their activity. Combined, these approaches may provide new insights into molecular mechanisms of lupus pathogenesis.

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Section: Resultssupporting

confidence: 76%