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Abstract

Differences in knotted1-like (knox) gene expression may account for some of the diversity of leaf forms seen in nature. Class 1 knox genes are expressed in the compound leaf primordia of tomato but not in the simple leaf primordia of a range of species examined so far. In order to test the hypothesis that all compound leaves differ from simple leaves in this way, we isolated a class 1 knox cDNA from pea, Pskn1 (Pisum sativum knotted1) and examined its expression pattern. The encoded homeodomain of Pskn1 shares 88% identical residues with KNOTTED1 from maize and an adjacent ELK domain is present. The protein sequence of PSKN1 is 69% identical to TKN2, its nearest related sequence in tomato. Unlike TKn2, Pskn1 was not expressed in newly initiated compound leaves. The expression pattern of Pskn1 resembled those of other class 1 knox genes described in maize and Arabidopsis. Transcripts were detected in the shoot apical meristem and developing vasculature of the vegetative shoot, but expression was not detected in newly initiated and developing compound leaf primordia. The same pattern of expression was observed in the afila mutant, which is characterised by highly ramified compound leaves. Our results suggest that tomato and pea use different developmental processes in the generation of their compound leaves.

Molecular dissection of the pea shoot apical meristem*

“…2B) consistent with the expression pattern previously reported by Hofer et al (2001); this is probably an indicator of undifferentiated cells in the SAM region, as reported for KN1 or STM in maize or Arabidopsis , respectively. Similar to Arabidopsis and maize, the expression of PsKN1 is down-regulated at leaf primordium initiation sites and this has also been shown by Hofer et al (2001) (Fig. 2B).…”

KNOTTED1-LIKE HOMEOBOX 3: a new regulator of symbiotic nodule development

“…In pea, only two genes of this family, KNOX1 (GenBank accession no. AF080104) and KNOX2 (AF080105), have been annotated in databases to date ( Hofer et al , 2001 ). To identify other pea KNOX genes, the recently created TSA (V. Zhukov, A. Zhernakov, O. Kulaeva, N. Ershov, A. Borisov and I. Tikhonovich, unpublished data) and other available TSAs of P. sativum L. ( Franssen et al , 2011 ; Kaur et al , 2012 ; Duarte et al , 2014 ) were searched using full-length KNOX1–KNOX10 cDNAs of M. truncatula .…”

“…In total, 10 KNOX genes have been identified in M. truncatula ( Di Giacomo et al , 2008 , Peng et al , 2011 ; Zhou et al , 2014 ). In pea ( P. sativum L.), two members of KNOX1 class, the KNOX1 and KNOX2 genes, have mainly been investigated and showed to be involved in the regulation of plant architecture ( Hofer et al , 2001 ; Zhou et al , 2014 ).…”

Genome-Wide Analysis of Homeobox Gene Family in Legumes: Identification, Gene Duplication and Expression Profiling

“…1 ) [ 7 ]. The ELK, KNOX A and KNOX B domains are required for nuclear localization, target gene suppression and homo-dimerization, respectively [ 45 , 46 ]. Notably, the number of TALE superclass proteins was significantly lesser (10) in Medicago as compared to other legumes ( Table 1 ).…”