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Hansen, Lise Bach; Siegismund, Hans R.; Jørgensen, Rikke Bagger

doi:10.1023/a:1013825816443

Cited by 260 publications

(7 citation statements)

References 16 publications

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“…Although many studies have investigated crossability between B. napus and B. rapa, many of them have been done with wild B. rapa (Jorgensen and Andersen 1994;Halfhill et al 2001Halfhill et al , 2002Hansen et al 2001;Snow et al 1999) or oilseed rape (Li et al 2006;Metz et al 1997;Zhao et al 2005), and very few studies have been done on the gene flow from transgenic rapeseed to various subspecies and varieties of B. rapa. The aim of this study was to quantify the gene transferability from B. napus to various subspecies and varieties of B. rapa and to estimate the germination ability of the interspecific hybrids.…”

Section: Introductionmentioning

confidence: 99%

Gene transferability from transgenic Brassica napus L. to various subspecies and varieties of Brassica rapa

Xiao

Zhang³

et al. 2009

Transgenic Res

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Gene transferability from transgenic rapeseed to various subspecies and varieties of Brassica rapa was assessed in this study. Artificial crossability was studied in 118 cultivars of 7 B. rapa subspecies and varieties with the transgenic rapeseed GT73 (Brassica napus) as the pollen donor. On average 5.7 seeds were obtained per pollination, with a range from 0.05 to 19.4. The heading type of B. rapa L. showed significantly higher crossability than non-heading types of B. rapa. The spontaneous outcrossing rate between B. rapa (female) and the transgenic rapeseed Ms8 x Rf3 (B. napus) (male) ranged from 0.039 to 0.406%, with an average of 0.19%. The fertilization process and the development of the hybrid seeds as shown by fluorescent staining techniques indicated that the number of adhered pollens on the stigma was reduced by 80%, the number of pollen tubes in the style was reduced by 2/3 and the fertilization time was delayed by over 20 h when pollinated with the transgenic rapeseed Ms8 x Rf3 in comparison with the bud self-pollination of B. rapa as control. About 10-70% of the interspecific hybrid embryos were aborted in the course of development. Some seeds looked cracked in mature pods, which showed germination abilities lower than 10%. The spontaneous outcrossing rates were much lower than the artificial crossability, and their survival fitness of the interspecific hybrid was very low, indicating that it should be possible to keep the adventitious presence of the off-plants under the allowed threshold, if proper measures are taken.

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Section: Introductionmentioning

confidence: 99%

Gene transferability from transgenic Brassica napus L. to various subspecies and varieties of Brassica rapa

Xiao

Zhang³

et al. 2009

Transgenic Res

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“…Spontaneous hybrids between OSR and B. rapa are known to occur under field conditions with either species as the pollen donor [105][106][107][108][109][110]. However, the transfer of herbicide-tolerant genes from OSR to B. rapa seems to vary considerably in agricultural environments (Tables 2, 3).…”

Section: Hybrids Of Oilseed Rape Becoming More Competitive Weeds In Amentioning

confidence: 99%

Ecological Impacts of Genetically Modified Crops: Ten Years of Field Research and Commercial Cultivation

Sanvido

Romeis

Bigler

Green Gene Technology

110

102

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“…Although "domestication" traits are typically considered unlikely to persist in wild populations, some traits may enhance hybrid fitness, especially in stressful environments (Mercer et al, 2007). Through molecular marker assays, the extensive transfer of nuclear as well as plastid DNA from oilseed rape into a self-maintained weedy population of B. rapa has been documented in agricultural conditions (Hansen et al, 2001). In addition, the persistence of the C-chromosome regions was identified in backcross progenies between B. juncea and B. napus (Frello et al, 1995;Tsuda et al, 2012;Guan et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Gene Flow Risks From Transgenic Herbicide-Tolerant Crops to Their Wild Relatives Can Be Mitigated by Utilizing Alien Chromosomes

et al. 2021

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Integration of a transgene into chromosomes of the C-genomes of oilseed rape (AACC, 2n = 38) may affect their gene flow to wild relatives, particularly Brassica juncea (AABB, 2n = 36). However, no empiric evidence exists in favor of the C-genome as a safer candidate for transformation. In the presence of herbicide selections, the first- to fourth-generation progenies of a B. juncea × glyphosate-tolerant oilseed rape cross [EPSPS gene insertion in the A-genome (Roundup Ready, event RT73)] showed more fitness than a B. juncea × glufosinate-tolerant oilseed rape cross [PAT gene insertion in the C-genome (Liberty Link, event HCN28)]. Karyotyping and fluorescence in situ hybridization–bacterial artificial chromosome (BAC-FISH) analyses showed that crossed progenies from the cultivars with transgenes located on either A- or C- chromosome were mixoploids, and their genomes converged over four generations to 2n = 36 (AABB) and 2n = 37 (AABB + C), respectively. Chromosome pairing of pollen mother cells was more irregular in the progenies from cultivar whose transgene located on C- than on A-chromosome, and the latter lost their C-genome-specific markers faster. Thus, transgene insertion into the different genomes of B. napus affects introgression under herbicide selection. This suggests that gene flow from transgenic crops to wild relatives could be mitigated by breeding transgenic allopolyploid crops, where the transgene is inserted into an alien chromosome.

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Cited by 260 publications

References 16 publications

Gene transferability from transgenic Brassica napus L. to various subspecies and varieties of Brassica rapa

Gene transferability from transgenic Brassica napus L. to various subspecies and varieties of Brassica rapa

Ecological Impacts of Genetically Modified Crops: Ten Years of Field Research and Commercial Cultivation

Gene Flow Risks From Transgenic Herbicide-Tolerant Crops to Their Wild Relatives Can Be Mitigated by Utilizing Alien Chromosomes

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