Untitled

Gomathinayagam, P.; Ram, S. Ganesh; RATHNASWAMY, R; Ramaswamy, N. M.

doi:10.1023/a:1018381614098

Cited by 37 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cowpea is highly susceptible to many viral diseases, such as Cowpea aphid borne mosaic virus, Cowpea severe mosaic virus, Blackeye cowpea mosaic virus and Cowpea yellow mosaic virus, and it is the host for a wide range of insect pests, notably flower bud thrips, pod borers, leaf beetles, pod suckers, aphids and leaf hoppers, which seriously limits the realization of its yield potential, estimated at 1.5-3.0 ton ha -1 (Murdock 1992;Aliyu 2007;Taiwo et al 2007). Durable and adequate levels of resistance to these viruses and pests are lacking in the primary gene pool, but are available in distant wild species, which present barriers for gene transfer through conventional crossing techniques (Gomathinayagam et al 1998). In addition, limited genetic diversity in cowpea breeding programs is of special concern because cowpea appears to have lower inherent genetic diversity than other cultivated crops as a result of a hypothesized single domestication event (Fang et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Biolistic-mediated genetic transformation of cowpea (Vigna unguiculata) and stable Mendelian inheritance of transgenes

et al. 2008

View full text Add to dashboard Cite

We describe a novel system of exploiting the biolistic process to generate stable transgenic cowpea (Vigna unguiculata) plants. The system is based on combining the use of the herbicide imazapyr to select transformed meristematic cells after physical introduction of the mutated ahas gene (coding for a mutated acetohydroxyacid synthase, under control of the ahas 5' regulatory sequence) and a simple tissue culture protocol. The gus gene (under control of the act2 promoter) was used as a reporter gene. The transformation frequency (defined as the total number of putative transgenic plants divided by the total number of embryonic axes bombarded) was 0.90%. Southern analyses showed the presence of both ahas and gus expression cassettes in all primary transgenic plants, and demonstrated one to three integrated copies of the transgenes into the genome. The progenies (first and second generations) of all self-fertilized transgenic lines revealed the presence of the transgenes (gus and ahas) co-segregated in a Mendelian fashion. Western blot analysis revealed that the GUS protein expressed in the transgenic plants had the same mass and isoelectric point as the bacterial native protein. This is the first report of biolistic-mediated cowpea transformation in which fertile transgenic plants transferred the foreign genes to next generations following Mendelian laws.

show abstract

Section: Introductionmentioning

confidence: 99%

Biolistic-mediated genetic transformation of cowpea (Vigna unguiculata) and stable Mendelian inheritance of transgenes

et al. 2008

View full text Add to dashboard Cite

show abstract

“…There have been earlier reports on the extent of genetic variability for quality traits in cowpea. [39] reported high methionine content in Tvu 2093 and Bush Sitao (3.24-3.4mg/g) dry seeds compared to 2.75-2.88mg/g seeds of the check variety, G-81-1. del.…”

Section: Annals Of Reviews and Researchmentioning

confidence: 94%

Breeding Cowpea Vignaunguiculata l. Walp for Quality Traits

Simion

2018

ARR

View full text Add to dashboard Cite

Cowpea is truly a multifunctional crop, providing food for man and livestock and serving as a valuable and dependable revenue-generating commodity for farmers and grain traders. Cowpea is of major importance to the livelihoods of millions of people in less developed countries of the tropics. It is consumed in many forms. Young leaves, green pods, and green seeds are used as vegetables, and dry seeds are used in various food preparations with 25% protein (on dry-weight basis) in its seeds and tender leaves, cowpea is a major source of protein, minerals, and vitamins in the daily diets in Africa, and thus it positively influences the health of men, women and children. The bulk of the diet of the rural and urban poor in Africa consists of starchy food made from cassava, yam, plantain and banana, millet, sorghum, and maize. The addition of even a small amount of cowpea ensures a nutritional balance and enhances the protein quality by the synergistic effect of high protein and high lysine from cowpea and high methionine and high energy from the starchy foods. Trading fresh produce and processed cowpea foods and snacks provides rural and urban women opportunity for earning cash income. A good knowledge of crop taxonomy contributes to an efficient use of germplasm for hybridization in the breeding programs. In addition, the results of hybridizations and cross compatibilities provide the basis for improving plant classification. Large and diverse cowpea germplasm collections are available to the researchers around the world, especially in Africa, to exploit the valuable genes to improve cowpea cultivars.

show abstract

“…oblonga , which is a salt-tolerant type, that could be further used in introducing salt tolerance in cultivated cowpea [ 41 ]. However, attempts to cross the cultivated African cowpea with salt-tolerant [ 117 ] V. vexillata , the closest species to cowpea [ 118 ], have so far proved fruitless [ 119 , 120 ] despite the creation and characterization of an interspecific hybrid created by Gomathinayagam et al [ 121 ]. As wild Vigna relatives are interesting material for the improvement of the cultivated Vigna [ 46 , 122 ], the continuation of the crossing effort is considered critical.…”

Section: The Exploitation Of the Cwrs In Specific Cultivated Speciesmentioning

confidence: 99%

Crop Wild Relatives: A Valuable Source of Tolerance to Various Abiotic Stresses

Kapazoglou¹,

Gerakari

Lazaridi

et al. 2023

Plants

View full text Add to dashboard Cite

Global climate change is one of the major constraints limiting plant growth, production, and sustainability worldwide. Moreover, breeding efforts of the past years have focused on improving certain favorable crop traits, leading to genetic bottlenecks. The use of crop wild relatives (CWRs) to expand genetic diversity and improve crop adaptability seems to be a promising and sustainable approach for crop improvement in the context of the ongoing climate challenges. In this review, we present the progress that has been achieved towards CWRs exploitation for enhanced resilience against major abiotic stressors (e.g., water deficiency, increased salinity, and extreme temperatures) in crops of high nutritional and economic value, such as tomato, legumes, and several woody perennial crops. The advances in -omics technologies have facilitated the elucidation of the molecular mechanisms that may underlie abiotic stress tolerance. Comparative analyses of whole genome sequencing (WGS) and transcriptomic profiling (RNA-seq) data between crops and their wild relative counterparts have unraveled important information with respect to the molecular basis of tolerance to abiotic stressors. These studies have uncovered genomic regions, specific stress-responsive genes, gene networks, and biochemical pathways associated with resilience to adverse conditions, such as heat, cold, drought, and salinity, and provide useful tools for the development of molecular markers to be used in breeding programs. CWRs constitute a highly valuable resource of genetic diversity, and by exploiting the full potential of this extended allele pool, new traits conferring abiotic-stress tolerance may be introgressed into cultivated varieties leading to superior and resilient genotypes. Future breeding programs may greatly benefit from CWRs utilization for overcoming crop production challenges arising from extreme environmental conditions.

show abstract

Untitled

Cited by 37 publications

References 18 publications

Biolistic-mediated genetic transformation of cowpea (Vigna unguiculata) and stable Mendelian inheritance of transgenes

Biolistic-mediated genetic transformation of cowpea (Vigna unguiculata) and stable Mendelian inheritance of transgenes

Breeding Cowpea Vignaunguiculata l. Walp for Quality Traits

Crop Wild Relatives: A Valuable Source of Tolerance to Various Abiotic Stresses

Contact Info

Product

Resources

About