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Sato, Takashi; Onoma, Noriyasu; Fujikake, Hiroyuki; Ohtake, Norikuni; Sueyoshi, Kuni; Ohyama, Takuji

doi:10.1023/a:1013317219871

Cited by 13 publications

(4 citation statements)

References 20 publications

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“…They started to fix nitrogen (N 2 ) at about 15 -20 days after planting when the diameter reached approximately 2 mm [45]. In the later stages, the nodules formed at the basal part of primary roots degrade, and a large number of new nodules formed on the lateral roots near the soil surface.…”

Section: Discussionmentioning

confidence: 99%

Low-Density Co-Inoculation of Myanmar Bradyrhizobium yuanmingense MAS34 and Streptomyces griseoflavus P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties

Soe¹,

Yamakawa²

2013

AJPS

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This study examined whether low-density co-inoculation of Myanmar Bradyrhizobium yuanmingense strain MAS34 and Streptomyces griseoflavus P4 would enhance nodulation, N 2 fixation, and seed yield in two soybean varieties. A field experiment was conducted during the July to November 2012 growing season at Kyushu University Farm, Japan, using a split-plot design with three replications and the following four treatments: T1, an uninoculated treatment with peat moss (uninoculated); T2, a single inoculation with S. griseoflavus P4 (P4); T3, a single inoculation of B. yuanmingense MAS34 (MAS34); and T4, a dual inoculation of P4 with MAS34 (P4 + MAS34). Two varieties of soybean, Yezin-3 (Rj 4 ) and Yezin-6 (non-Rj), were used. The N 2 fixation activity of soybean was evaluated by the relative ureide method using xylem solute from root bled sap at the early pod-fill stage (R3.5). Dry matter production, N 2 fixation, and seed yield were significantly (P < 0.01) different between the inoculated treatments. The effect of variety was also significant (P < 0.05) for nodule dry weight at the V6 stage, percentage of N derived from the atmosphere at the R3.5 stage, and seed yield at the maturity stage. The number of nodules on the tap roots was significantly higher in Yezin-3 than in Yezin-6. The single inoculation of P4 did not have a significant effect on dry matter production, N 2 fixation, and seed yield in either soybean variety. The dry matter production, relative ureide index, percentage of N derived from the atmosphere, and seed yield were significantly (P < 0.01) enhanced by a single inoculation of MAS34 in Yezin-3 and by dual inoculation of P4 + MAS34 in Yezin-6. These results indicate that low inoculum concentrations (10 5 cells seed −1 ) increase N 2 fixation and seed yield in these soybean varieties under open field conditions. Myanmar B. yuanmingense MAS34 and S. griseoflavus P4 are expected to be useful biofertilizers for soybean production.

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Section: Discussionmentioning

confidence: 99%

Low-Density Co-Inoculation of Myanmar Bradyrhizobium yuanmingense MAS34 and Streptomyces griseoflavus P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties

Soe¹,

Yamakawa²

2013

AJPS

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“…However, Lombin et al [ 23 ] concluded that nitrogen fertilization had no effect on the yield increase in peanut. The results of this experiment showed that both 25% and 35% N reduction reduced the main stem height, leaf area index, SPAD value and net photosynthetic rate of peanut; in the middle and late stages of fertility, additional organic fertilizer increased the net photosynthetic rate of leaves, and the net photosynthetic rate and SPAD of T3 and T4 treatments were the highest under the synergistic application of N fertilizer and organic fertilizer, which may be explained as follows: N in chemical fertilizer can be released rapidly and thus directly replenish the soil N The nutrients in bio-organic fertilizers need to be transformed by microbial processes before they can be absorbed by plants, and this process is longer than that of chemical fertilizers, therefore, the physiological indexes of peanut decreased rapidly under the rapid nitrogen reduction treatment, while in the middle and late stages, the photosynthetic efficiency and yield of peanut were improved under the treatment of additional organic fertilizers [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Reducing Nitrogen Fertilization and Adding Organic Fertilizer on Net Photosynthetic Rate, Root Nodules and Yield in Peanut

Zhang¹,

Liu

Zhang³

et al. 2023

Plants

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Long-term excessive application of chemical fertilizers can cause many problems, such as soil degradation and environmental pollution. Therefore, we reduced conventional nitrogen fertilization and added organic fertilizers in some cases to investigate the response of photosynthetic characteristics, root nodules and yield on reduced nitrogen fertilization. Compared to conventional nitrogen fertilization, the 25% and 35% nitrogen reduction treatments reduced the leaf area index, net photosynthetic rate, 100-fruit weight, 100-kernel weight and the yield of peanut, but had no significant effect on the kernel rate. With constant N fertilizer, adding organic fertilization alone increased leaf area index, chlorophyll, net photosynthetic rate and yield of peanut. In compounded treatments of nitrogen and organic fertilizer, the highest yields were achieved in the 25% N reduction with the 3000 kg/hm−2 organic fertilizer treatment (T3) and the 4500 kg/hm−2 organic fertilizer treatment (T4); furthermore, the net photosynthetic rate, leaf area index, yield and fertilizer contribution were significantly higher in these two treatments than in the conventional fertilizer treatments. Nitrogen fertilizer had significant effects on the quantity and fresh weight of root nodules. Concretely, nitrogen reduction increased the quantity and fresh weight of root nodules of peanut in the early stage of fertility but decreased them in the harvest stage. Nitrogen reduction with an additional organic fertilizer in the late stage of fertility increased the quantity and fresh weight of root nodules of peanut. Considering the property of root nodules was significantly positively correlated with net photosynthetic rate and yield, the arguments above may be the mechanism of the highest yields found in T3 and T4. This work can provide empirical and instructional support for a balanced fertilization strategy in peanut agriculture and high-yielding and efficient cultivation of peanut.

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“…The heterogeneity of samples of ricin toxins extracted from five horticultural varieties of Ricinus communis seeds was evaluated [60], and the four major leghemoglobin components, Lba, Lbc1, Lbc2 and Lbc3 from soybean nodules were quantified [61]. The pI of a biologically active lectin from Korean mistletoe, Viscum album var.…”

Section: Plant Proteinsmentioning

confidence: 99%

Recent applications of capillary isoelectric focusing

Kilár

2003

Electrophoresis

101

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After the advent of capillary isoelectric focusing (CIEF) in the 80's several approaches have been developed in order to use the technique in routine analyses. The recent years showed an extensive increase in the applications of this technique employing its exceptionally high-resolution power. Methodological improvements, as well as hyphenation with other electrophoretic and chromatographic separation procedures, proved the versatility of CIEF in studies of clinically important proteins, recombinant product, cell lysates and other complex mixtures. The combination of CIEF with mass spectrometry detection is one of the major challenges for studying proteomics. This review collected the recent applications of CIEF including innovations in the experimental setup, remedies for the presence of salts in samples, calibration of the pH gradient, carrier ampholyte-free isoelectric focusing, the progress in micropreparation, two-dimensional separations, etc.

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Untitled

Cited by 13 publications

References 20 publications

Low-Density Co-Inoculation of Myanmar <i>Bradyrhizobium yuanmingense</i> MAS34 and <i>Streptomyces griseoflavus</i> P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties

Low-Density Co-Inoculation of Myanmar <i>Bradyrhizobium yuanmingense</i> MAS34 and <i>Streptomyces griseoflavus</i> P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties

Effect of Reducing Nitrogen Fertilization and Adding Organic Fertilizer on Net Photosynthetic Rate, Root Nodules and Yield in Peanut

Recent applications of capillary isoelectric focusing

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Untitled

Cited by 13 publications

References 20 publications

Low-Density Co-Inoculation of Myanmar &lt;i&gt;Bradyrhizobium yuanmingense&lt;/i&gt; MAS34 and &lt;i&gt;Streptomyces griseoflavus&lt;/i&gt; P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties

Low-Density Co-Inoculation of Myanmar &lt;i&gt;Bradyrhizobium yuanmingense&lt;/i&gt; MAS34 and &lt;i&gt;Streptomyces griseoflavus&lt;/i&gt; P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties

Effect of Reducing Nitrogen Fertilization and Adding Organic Fertilizer on Net Photosynthetic Rate, Root Nodules and Yield in Peanut

Recent applications of capillary isoelectric focusing

Contact Info

Product

Resources

About

Low-Density Co-Inoculation of Myanmar <i>Bradyrhizobium yuanmingense</i> MAS34 and <i>Streptomyces griseoflavus</i> P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties

Low-Density Co-Inoculation of Myanmar <i>Bradyrhizobium yuanmingense</i> MAS34 and <i>Streptomyces griseoflavus</i> P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties