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Singh, Chandra Jeet

doi:10.1023/a:1023395409746

Cited by 61 publications

(6 citation statements)

References 36 publications

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“…strong mechanical destruction combined with lysis, produce the effect of keratinolysis (Kunert 2000; Mitola et al 2002). The reserve effect was noted in studies by Singh (2002) where a high activity of fungal keratinases was correlated with weak solubilization of keratin substrate. The authors attribute this to the removal or repression of some accessory proteins required by keratinase to act effectively on the keratin molecule while splitting its disulfide bonds.…”

Section: Discussionmentioning

confidence: 84%

“…For those reasons the fungi constitute an important group among the destruents of organic matter in the soil and, as reported by Singh (2002), in the bioremediation of environments polluted with keratin waste. According to Vasileva-Tonkova et al (2009) and Syed et al (2009), microbiological degradation of keratin waste is also an ecologically and economically safe method of utilization of such waste.…”

Section: Discussionmentioning

confidence: 90%

“…Singh (2002) reports that the degree of keratin degradation correlated with the changes in enzymatic activity is the direct evidence of keratinolytic abilities of fungi. Investigations conducted in the present paper show that the biodegradation of chicken feathers by five different strains occurs with active keratinases and proteases.…”

Section: Discussionmentioning

confidence: 99%

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Biodegradation of feather waste keratin by a keratinolytic soil fungus of the genus Chrysosporium and statistical optimization of feather mass loss

Bohacz

2016

World J Microbiol Biotechnol

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This paper assesses the ability of strains of Aphanoascus fulvescens and Chrysosporium articulatum isolated from soil (phaesol) to degrade native feather keratin. Strains were identified based on phenotypic traits and nucleotide sequencing. Response Surface Methodology was used to optimize cultivation conditions exhibiting the highest keratinolytic activity. The experiments were based on Box-Behnken designs for the loss of substrate mass (chicken feathers). While substrate mass loss is an “economic coefficient” that reliably indicates feather keratin degradation, it has not been studied before. Stationary liquid cultures of five selected strains were conducted in laboratory conditions at 28 °C using poultry feathers (1 g) as the sole source of carbon, nitrogen and energy. Enzymatic activities, keratin mineralization products and substrate mass loss were determined periodically. The mineralization of keratin proteins by strains yielded a high number of ammonium ions alkalinizing the medium. Increased ammonium ions inhibited the activity of caseinian protease and keratinase. A decrease in the concentration of these ions induced proteolytic enzymes, chiefly the activity of keratinase, at the end of fungal cultivation. Keratinase activity was related to protein- and peptide release and that of caseinian protease to sulfate ions. The highest loss of substrate mass in comparison to the reference strain CBS104.62 (35.4%) was recorded for Aphanoascus fulvescens B21/4-5 (65.9%). Based on a Box-Behnken design, the maximum loss of substrate mass for the Aphanoascus fulvescens strain (71.08%) can be achieved at pH 7.58 and temperature 28.7 °C.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 90%

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Biodegradation of feather waste keratin by a keratinolytic soil fungus of the genus Chrysosporium and statistical optimization of feather mass loss

Bohacz

2016

World J Microbiol Biotechnol

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“…3.4.99.11), peptidases which are capable of degrading keratin. Various authors have reported that, among the keratinolytic microorganisms, some species of Bacillus [10–12], actinomycetes [9, 13, 14], and fungi [15–17] are able to produce these keratinases and peptidases. …”

Section: Introductionmentioning

confidence: 99%

Keratinase Production by ThreeBacillusspp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation

et al. 2011

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Three Bacillus species (B. subtilis LFB-FIOCRUZ 1270, B. subtilis LFB-FIOCRUZ 1273, and B. licheniformis LFB-FIOCRUZ 1274), isolated from the poultry industry, were evaluated for keratinase production using feathers or feather meal as the sole carbon and nitrogen sources in a submerged fermentation. The three Bacillus spp. produced extracellular keratinases and peptidases after 7 days. Feather meal was the best substrate for keratinase and peptidase production in B. subtilis 1273, with 412 U/mL and 463 U/ml. The three strains were able to degrade feather meal (62–75%) and feather (40–95%) producing 3.9–4.4 mg/ml of soluble protein in feather meal medium and 1.9–3.3 mg/ml when feather medium was used. The three strains produced serine peptidases with keratinase and gelatinase activity. B. subtilis 1273 was the strain which exhibited the highest enzymatic activity.

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“…Las queratinasas por su amplio espectro de acción tienen numerosas aplicaciones en diversas industrias: biomedicina, bioenergía, textil, medicina, cosmética, cuero, bioconversión de residuos queratínicos Singh, 2002).…”

Section: Aplicaciones Biotecnológicas De Las Queratinasasunclassified

Valorización de residuos sólidos de curtiembre: biotransformación fúngica del pelo vacuno

Galarza

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El presente trabajo de Tesis Doctoral trata la revalorización de uno de los residuos sólidos generados en la industria del cuero local, el residuo pelo (RP). Este residuo se produce a partir del cambio en el proceso de depilado hacia una tecnología más limpia, el “depilado conservador del pelo”. El pelo mantiene sus propiedades de fibra y se convierte en un residuo sólido de naturaleza proteica refractario a la degradación. En relación al depilado tradicional o destructor implica la reducción de la carga orgánica del efluente en un 40% a 60% en términos de DQO, la disminución de la concentración de sulfuro en un 50% y de los sólidos suspendidos sedimentables en un 75%. La magnitud de su generación alcanza el 10 % al 20 % en peso de las pieles saladas, por lo que una curtiembre mediana produce 1 t por día. La valorización de este residuo mediante la biotransformación comenzó con el aislamiento de cepas fúngicas de suelo y de muestras clínicas causantes de onicomicosis. Se determinaron sus actividades proteolíticas, queratinolíticas, colagenolíticas a partir de sus extractos crudos obtenidos tanto en medio líquido como en sólido, usando el RP como sustrato para el cultivo. Se seleccionó a Trichophyton ajelloi. Se estudió la optimización en las condiciones de cultivo y de las distintas actividades de su extracto enzimático en diferentes condiciones físico-químicas, mediante la adaptación y puesta a punto de protocolos, en especial de la actividad queratinolítica con RP. La degradación fúngica se puso en evidencia mediante técnicas de Microscopía Electrónico de Barrido y de Transmisión, Espectroscopía Dieléctrica y colorimétricas. Se aplicó el extracto enzimático a escala piloto en la etapa de remojo, depilado y purga de pieles bovinas en Tecnología del Cuero. Estos ensayos de actividad queratinolítica in vitro e in vivo de económica implementación constituyen un paquete tecnológico para predecir la acción enzimática en el depilado en pos de producir cueros de calidad y sustituir importaciones para la industria curtidora local.

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Untitled

Cited by 61 publications

References 36 publications

Biodegradation of feather waste keratin by a keratinolytic soil fungus of the genus Chrysosporium and statistical optimization of feather mass loss

Biodegradation of feather waste keratin by a keratinolytic soil fungus of the genus Chrysosporium and statistical optimization of feather mass loss

Keratinase Production by ThreeBacillusspp. Using Feather Meal and Whole Feather as Substrate in a Submerged Fermentation

Valorización de residuos sólidos de curtiembre: biotransformación fúngica del pelo vacuno

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