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Vasyukova, N. I.; Зиновьева, С. В.; Udalova, Zh. V.; Panina, Ya. S.; Ozeretskovskaya, O. L.; Sonin, M. D.

doi:10.1023/a:1025158702692

Cited by 20 publications

(5 citation statements)

References 8 publications

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“…Our bioassays show that chitosan induces root exudates inhibitory to root pathogenic fungus FORL and root-knot nematode eggs without significantly affecting the growth and development of a biocontrol fungus (Pc). The toxic effect of chitosan-derived exudates may be related to the overproduction of SA which, in combination with chitosan induce systemic acquired resistance and reduce infection by root-knot nematodes ( Vasyukova et al, 2003 ; Singh et al, 2020 ). Chitosan, moreover, by itself, is toxic to fungi such as Fusarium spp.…”

Section: Discussionmentioning

confidence: 99%

Chitosan Induces Plant Hormones and Defenses in Tomato Root Exudates

et al. 2020

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In this work, we use electrophysiological and metabolomic tools to determine the role of chitosan as plant defense elicitor in soil for preventing or manage root pests and diseases sustainably. Root exudates include a wide variety of molecules that plants and root microbiota use to communicate in the rhizosphere. Tomato plants were treated with chitosan. Root exudates from tomato plants were analyzed at 3, 10, 20, and 30 days after planting (dap). We found, using high performance liquid chromatography (HPLC) and excitation emission matrix (EEM) fluorescence, that chitosan induces plant hormones, lipid signaling and defense compounds in tomato root exudates, including phenolics. High doses of chitosan induce membrane depolarization and affect membrane integrity. 1 H-NMR showed the dynamic of exudation, detecting the largest number of signals in 20 dap root exudates. Root exudates from plants irrigated with chitosan inhibit ca. twofold growth kinetics of the tomato root parasitic fungus Fusarium oxysporum f. sp. radicis-lycopersici. and reduced ca. 1.5-fold egg hatching of the root-knot nematode Meloidogyne javanica .

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Section: Discussionmentioning

confidence: 99%

Chitosan Induces Plant Hormones and Defenses in Tomato Root Exudates

et al. 2020

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“…The role of specific components of SA regulation, synthesis, and signaling in defending plants against parasitic nematodes is not well understood. However, SA does play a role in decreasing susceptibility to root-knot nematode (RKN) in cow pea [19] and tomato [20,21], and to the cyst nematode, Heterodera schachtii, in Arabidopsis [10]. Likewise, JA also plays a role in resistance of plants to nematodes.…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis genes, AtNPR1, AtTGA2 and AtPR-5, confer partial resistance to soybean cyst nematode (Heterodera glycines) when overexpressed in transgenic soybean roots

et al. 2014

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BackgroundExtensive studies using the model system Arabidopsis thaliana to elucidate plant defense signaling and pathway networks indicate that salicylic acid (SA) is the key hormone triggering the plant defense response against biotrophic and hemi-biotrophic pathogens, while jasmonic acid (JA) and derivatives are critical to the defense response against necrotrophic pathogens. Several reports demonstrate that SA limits nematode reproduction.ResultsHere we translate knowledge gained from studies using Arabidopsis to soybean. The ability of thirty-one Arabidopsis genes encoding important components of SA and JA synthesis and signaling in conferring resistance to soybean cyst nematode (SCN: Heterodera glycines) are investigated. We demonstrate that overexpression of three of thirty-one Arabidoposis genes in transgenic soybean roots of composite plants decreased the number of cysts formed by SCN to less than 50% of those found on control roots, namely AtNPR1(33%), AtTGA2 (38%), and AtPR-5 (38%). Three additional Arabidopsis genes decreased the number of SCN cysts by 40% or more: AtACBP3 (53% of the control value), AtACD2 (55%), and AtCM-3 (57%). Other genes having less or no effect included AtEDS5 (77%), AtNDR1 (82%), AtEDS1 (107%), and AtPR-1 (80%), as compared to control. Overexpression of AtDND1 greatly increased susceptibility as indicated by a large increase in the number of SCN cysts (175% of control).ConclusionsKnowledge of the pathogen defense system gained from studies of the model system, Arabidopsis, can be directly translated to soybean through direct overexpression of Arabidopsis genes. When the genes, AtNPR1, AtGA2, and AtPR-5, encoding specific components involved in SA regulation, synthesis, and signaling, are overexpressed in soybean roots, resistance to SCN is enhanced. This demonstrates functional compatibility of some Arabidopsis genes with soybean and identifies genes that may be used to engineer resistance to nematodes.

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“…SA is a known inhibitor of root and leaf CAT [ 32 ]. SA was found to be overproduced in roots and shoots of resistant tomato attacked by M. incognita [ 33 ]. Actually, most of the SA produced in roots may rapidly be transferred to leaves, in analogy with the SA adsorbed by roots from a solution [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Epigenetic and Metabolic Changes in Root-Knot Nematode-Plant Interactions

Leonetti

Molinari

2020

IJMS

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Two wild-type field populations of root-knot nematodes (Mi-Vfield, Mj-TunC2field), and two isolates selected for virulence in laboratory on resistant tomato cultivars (SM2V, SM11C2), were used to induce a resistance reaction in tomato to the soil-borne parasites. Epigenetic and metabolic mechanisms of resistance were detected and compared with those occurring in partially or fully successful infections. The activated epigenetic mechanisms in plant resistance, as opposed to those activated in infected plants, were detected by analyzing the methylated status of total DNA, by ELISA methods, and the expression level of key genes involved in the methylation pathway, by qRT-PCR. DNA hypo-methylation and down-regulation of two methyl-transferase genes (CMT2, DRM5), characterized the only true resistant reaction obtained by inoculating the Mi-1.2-carrying resistant tomato cv Rossol with the avirulent field population Mi-Vfield. On the contrary, in the roots into which nematodes were allowed to develop and reproduce, total DNA was generally found to be hyper-methylated and methyl-transferase genes up-loaded. DNA hypo-methylation was considered to be the upstream mechanism that triggers the general gene over-expression observed in plant resistance. Gene silencing induced by nematodes may be obtained through DNA hyper-methylation and methyl-transferase gene activation. Plant resistance is also characterized by an inhibition of the anti-oxidant enzyme system and activation of the defense enzyme chitinase, as opposed to the activation of such a system and inhibition of the defense enzyme glucanase in roots infested by nematodes.

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Cited by 20 publications

References 8 publications

Chitosan Induces Plant Hormones and Defenses in Tomato Root Exudates

Chitosan Induces Plant Hormones and Defenses in Tomato Root Exudates

Arabidopsis genes, AtNPR1, AtTGA2 and AtPR-5, confer partial resistance to soybean cyst nematode (Heterodera glycines) when overexpressed in transgenic soybean roots

Epigenetic and Metabolic Changes in Root-Knot Nematode-Plant Interactions

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