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Abstract

Fixed-dose combinations have several advantages comparing with a single drug and separate agents. For simple dosing regimens and their synergistic antihypertensive action, a combination of hydrochlorothiazide and losartan potassium is widely prescribed. In this study, an HPLC-UV method was developed and validated for the simultaneous determination of hydrochlorothiazide and losartan in bulk and pharmaceutical formulation. The method was optimized selecting chromatographic conditions of 60: 40 acetonitrile: water, ACE3-C18 column (250 mm × 4.6 mm 5 µm), 20 µl injection volume, the flow rate of 1 ml/min at ambient temperature (25 °C), and 226 nm. The method was validated giving good precision (RSD% < 1), acceptable linearity (R2 ≥ 0.997), and low LOD and LOQ (0.5 and 1.5 µg/ml, respectively). Successful application on pharmaceutical dosage tablet form gave recovery percent within acceptance criteria (92% and above) indicating that the proposed method is simple and reliable for the determination of LOP and HCTZ and hence can be applied for routine analysis in quality control laboratories.

Development and validation of first and zero order derivative spectrophotometric methods for the determination of rilmenidine in pharmaceutical preparations and forced degradation study

“…[1][2][3][4][5][6][7] Today, there are various drug treatment classifications used in the treatment of hypertension, commonly referred to as antihypertensive drugs. [8][9][10][11] Antihypertensive drugs were categorized into six separate drug groups: Angiotensin converting enzyme (ACE)-inhibitors, Angiotensin-receptor blockers (ATR-blockers), diuretics, beta-blockers, calciumchannel blockers and other antihypertensive drugs. [12] Rilmenidine is an antihypertensive agent with selectivity for I1 imidazoline receptors that acts both centrally by reducing sympathetic overactivity and in the kidney by inhibiting the Na + /H + antiport.…”