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Ahmad, Annisa Farhana; Sulaeman, Sulaeman; Mulia, Yuliansyah Sundara; O.W, Samidjo Jangkung

doi:10.34011/juriskesbdg.v11i1.780

Cited by 2 publications

(3 citation statements)

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“…Carbohydrates have an essential function in the growth of fungi, and proteins function as structural molecules that are useful in forming cell layers, functional molecules, and enzymes and help the metabolic process (Wantini & Octavia, 2018). Mushrooms will use the carbohydrate content found in green beans in excreting enzymes α-amylase to convert amylum into glucose, whereas fungi will absorb glucose to assist in the growth process (Ahmad et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

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Utilization of Green Beans Vima 1 and Local as an Alternative Media Substitute Sabouraud Dextrose Agar (SDA) in the Growth of Trichophyton Rubrum

Widyanur,

Sasongkowati,

Suliati

2023

JHS

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Green beans are cultivated plants and crops with nutrients from vegetable protein. Varieties of mung beans vary, including Vima 1 and local. The fungus Trichophyton rubrum generates dermatophytosis. This research explored green beans and local Vima 1 as an alternative medium in the growth of Trichophyton rubrum fungus. The type of research used by laboratory experiments was carried out at the STIKes Hutama Abdi Husada Tulungagung Microbiology Laboratory in March - April 2022. SDA media and alternative media green beans Vima 1 and local variations of the mass of 6 grams and 7 grams inoculated Trichophyton rubrum fungus using a single dot method with repetition as much as five times. The results showed that Trichophyton rubrum fungus grows optimally on alternative media green beans Vima 1 variation of 7 grams of mass that grows colonies on the fifth day with an average final diameter of 14 mm. While alternative media green beans Vima 1 variation mass 6 grams 11 mm, SDA media 11.65 mm, alternative media local green beans variation mass 6 grams 7.5 mm, and variation mass 7 grams 11.5 mm. The result of independent statistical tests was a p-value < 0.00, which shows significant differences in each type of media. This study result concluded that green beans Vima 1 and local could be used as an alternative medium to replace the SDA media in the growth of Trichophyton rubrum fungus.

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Section: Discussionmentioning

confidence: 99%

“…Inoculation of the fungus Trichophyton rubrum uses the single dot method. This method is done by inserting a needle nose in the middle of the surface so that the media SDA, green bean media varieties Vima 1 and local variations of the mass of 6 grams and 7 grams (Ahmad et al, 2019).…”

Section: Fungi Inoculation Into Alternative Media Sourcementioning

confidence: 99%

Utilization of Green Beans Vima 1 and Local as an Alternative Media Substitute Sabouraud Dextrose Agar (SDA) in the Growth of Trichophyton Rubrum

Widyanur,

Sasongkowati,

Suliati

2023

JHS

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“…Penilaian kualitas pertumbuhan koloni Trichopyton rubrum dapat dilakukan secara makroskopis dan mikroskopis. Secara makroskopis, pertumbuhan koloni Trichopyton rubrum dapat diamati melalui peningkatan diameter koloni yang semakin bulat dan membesar pada media PDA sebagai kontrol maupun pada media nasi aking seiring dengan peningkatan massa tepung 22 .…”

Section: Pembahasanunclassified

PEMANFAATAN NASI AKING SEBAGAI PENGGANTI SUMBER KARBOHIDRAT PADA MEDIA Potato Dextrose Agar (PDA) UNTUK PERTUMBUHAN Trichopyton rubrum

Hartini Analis,

Rohayati,

Rahmat

et al. 2023

JKS

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Dermatofitosis merupakan infeksi jamur dari kelompok dermatofita. Salah satu jenis infeksi yangumum terjadi adalah tinea pedis yang disebabkan oleh Trichopyton rubrum. Jamur ini melekatpada kulit dan menggunakan enzim keratinase untuk mencerna keratin. Dalam mendiagnosisinfeksi jamur ini, dilakukan pengkulturan pada media PDA. Sumber karbohidrat dalam media PDAdapat digantikan dengan bahan lain yang lebih ekonomis dan mudah diperoleh seperti nasi aking.Nasi aking mengandung karbohidrat sebesar 83,14%, protein 3,36% serta amilosa 29,70%.Penelitian ini menggunakan desain quasi eksperiment, dengan mengggunakan 3 replikasi dan 2perlakuan yaitu variasi massa tepung (5g, 10g, dan 15g), serta lama waktu inkubasi (Hari ke-3,5, 7, dan 10). Media PDA digunakan sebagai kontrol. Inokulasi Trichopyton rubrum dilakukandengan metode single dot pada media PDA dan media nasi aking. Pengamatan dilakukan secaramakroskopis dengan mengamati karakteristik morfologi dan mengukur diameternya, serta secaramikroskopis untuk melihat adanya hifa, makrokonidia, dan mikrokonidia. Hasil data pengukuran diameter pertumbuhan Trichopyton rubrum dianalisis menggunakan uji parametrik Two Way Anova. Dari penelitian dapat disimpulkan bahwa pertumbuhan Trichopyton rubrum pada mediaPDA dan media nasi aking tidak menunjukkan perbedaan yang signifikan. Rata-rata diameterkoloni Trichopyton rubrum mencapai 6,20 cm pada konsentrasi tepung nasi aking sebesar 10gram dengan waktu inkubasi 10 hari. Dengan demikian, nasi aking dapat digunakan sebagaipengganti karbohidrat dalam media PDA untuk pertumbuhan Trichopyton rubrum.

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Cited by 2 publications

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Utilization of Green Beans Vima 1 and Local as an Alternative Media Substitute Sabouraud Dextrose Agar (SDA) in the Growth of Trichophyton Rubrum

Utilization of Green Beans Vima 1 and Local as an Alternative Media Substitute Sabouraud Dextrose Agar (SDA) in the Growth of Trichophyton Rubrum

PEMANFAATAN NASI AKING SEBAGAI PENGGANTI SUMBER KARBOHIDRAT PADA MEDIA Potato Dextrose Agar (PDA) UNTUK PERTUMBUHAN Trichopyton rubrum

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