The work is devoted to the study of the composition of polyunsaturated fatty acids (PUFA) at artemia of different stages of ontogenesis. The content of PUFA in intact cysts and decapsulated artemia eggs was studied, and the content of PUFA after enrichment of cysts, decapsulated eggs, and artemia nauplia with a multicomponent complex of biologically active substances was also studied. The composition of the enriching complex included probiotics, adaptogens, vitamins, amino acids and hemp oil. The results of the research showed that the use of the enriching complex significantly increased the content of polyunsaturated fatty acids of the omega–3 and omega-6 families. The content of eicosapentaenoic, docosahexaenoic, linolenic fatty acids from Omega-3 family, and the content of linoleic and arachidonic acids from Omega-6 family increased to the greatest extent, primarily in nauplia, decapsulated eggs and artemia cysts, thereby significantly increasing their biological and nutritional value while using as starter feeds for fish.