Untitled

Abstract

A reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of Atenolol (ATN) in bulk and pharmaceutical formulation. The method was optimized selecting chromatographic conditions of 60: 40 acetonitrile: water, Inertsil_ column(ODS-3 250 mm × 4.6 mm 5 m), 20 L injection volume, flow rate of 1 mL/min at ambient temperature (30 °C), and 276 nm. Using two HPLC systems of first HPLC system coupled with PDA detector and the second HPLC system coupled with UV detector showed no big difference in the method results. The method was validated giving good precision (RSD% < 1), acceptable linearity (R 2 > 0.998), and low LOD and LOQ (0.5 and 1.5 g/mL, respectively) on both systems. Successful application on pharmaceutical dosage tablet form gave high recovery of 97.7%. The proposed method is economic, simple, and rapid and hence can be employed for routine analysis in quality control laboratories.