Untitled

Morphological traits and genetic diversity of differently overwintered oilseed rape (Brassica napus L.) cultivars

“…In our previous work, we screened 20 primers for their suitability for analysis of oilseed rape polymorphism. Eight primers 222,250,268,269,340,474,516,563 were chosen according to Dulson et al (1998), these primers were proved suitable for oilseed rape polymorphism detection Paulauskas et al, 2008). The other four primers OPA 1, OPA 4, OPA 9, OPA 11 were used earlier in the detection of polymorphism in Brassica napus and other plants Paulauskas et al, 2008).…”

“…The highest amount of population-specific bands was identified in the cultivar 'DK Secure' -5 private bands (268 650 , OPA 9 800, 850, 1200, 1500 ), in cultivar 'SW Celsius' -3 private bands (222 300 , 474 620 , OPA 9 600 ), 2 private bands were found in cultivars 'Titan' and 'Libea'. Dulson et al (1998) analyzed in detail some of the primers used for bulked DNA samples for RAPD DNA fingerprinting in B. napus cultivars. Some of the markers mentioned were also found in our analysis -according to Dulson et al (1998) 250.0825 was invariant in abundance -it had the same invariance in all tested cultivars except for 'DK Secure' where it was absent.…”

“…Dulson et al (1998) analyzed in detail some of the primers used for bulked DNA samples for RAPD DNA fingerprinting in B. napus cultivars. Some of the markers mentioned were also found in our analysis -according to Dulson et al (1998) 250.0825 was invariant in abundance -it had the same invariance in all tested cultivars except for 'DK Secure' where it was absent. 269.0600 was detected in three of the four tested cultivars (Dulson et al, 1998), and the same was detected in all 11 tested cultivars; some markers -222.1500, 474.0700, 268.0600 were amplified only in some of the tested winter oilseed rape cultivars.…”

“…Primers used were synthesized by Fermentas Thermo Scientific (Lithuania). Twelve primers were screened for their ability to produce polymorphic patterns: 222,250,268,269,340,474,516,563, OPA 1, OPA 4, OPA 9 and OPA 11 (Dulson et al, 1998;Paulauskas et al, 2008). Nine of them were selected for further experiments.…”

“…RAPD markers are used for genetic diversity analysis of many plant species (Galović et al, 2006;Vyšniauskienė et al, 2011;Zybartaite et al, 2011;Vyšniauskienė et al, 2013). RAPD markers were used for evaluation of genetic variability in some Brassica species (Dulson et al, 1998;Yu et al, 2005). Sobotka et al (2004) concluded that AFLP method detected polymorphisms in oilseed rape cultivars more efficiently than either RAPD or SSR methods, but also stated the importance to detect informative markers and confirmed that the results obtained by different marker systems were often similar.…”