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Hwang, Seongsoo; Lee, Eun-Young; Yoon, Jong Taek; Yoon, Byung Koo; Lee, Je‐Ho; Choi, Dukhyun

doi:10.1023/a:1009496726343

Cited by 27 publications

(3 citation statements)

References 17 publications

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“…Chemical or mechanical activation of the oocyte after ICSI has been commonly done in cattle. In that regard, use of electric stimulation [ 109 ], mechanical pre-treatment [ 80 ], or chemicals such as ethanol [ 110 ], ionomycin, anisomycin (ANY), cycloheximide (CHX), DMAP, dehydroleucodine (DhL) [ 111 ] independently or in various combinations, have been reported. Oocyte activation with ethanol alone or in combination with cycloheximide [ 112 , 113 ] or ionomycin [ 92 , 114 ] following ICSI resulted in development of blastocysts.…”

Section: Artificial Activation Of Oocyte For Improving the Successmentioning

confidence: 99%

Intracytoplasmic Sperm Injection in Cattle

Unnikrishnan

Kastelic

Thundathil

2021

Genes

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Intracytoplasmic sperm injection (ICSI) involves the microinjection of sperm into a matured oocyte. Although this reproductive technology is successfully used in humans and many animal species, the efficiency of this procedure is low in the bovine species mainly due to failed oocyte activation following sperm microinjection. This review discusses various reasons for the low efficiency of ICSI in cattle, potential solutions, and future directions for research in this area, emphasizing the contributions of testis-specific isoforms of Na/K-ATPase (ATP1A4) and phospholipase C zeta (PLC ζ). Improving the efficiency of bovine ICSI would benefit the cattle breeding industries by effectively utilizing semen from elite sires at their earliest possible age.

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Section: Artificial Activation Of Oocyte For Improving the Successmentioning

confidence: 99%

Intracytoplasmic Sperm Injection in Cattle

Unnikrishnan

Kastelic

Thundathil

2021

Genes

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“…To overcome this problem, bovine ICSI oocytes have been treated to induce intracellular calcium spike with a direct current pulse(s) (Hwang et al 2000), calcium ionophore (Keskintepe & Brackett 2000), ionomycin (Rho et al 1998; Galli et al 2003; Oikawa et al 2005; Abdalla et al 2009) or ethanol (Horiuchi et al 2002; Oikawa et al 2005; Abdalla et al 2009). Because these stimuli do not induce long‐lasting oscillations, they have been combined with other chemicals, such as cycloheximide (Galli et al 2003) or 6‐dimethylaminopurine (Rho et al 1998; Oikawa et al 2005) that interfere with either the re‐synthesis or the activation of the metaphase‐promoting factor (MPF), respectively.…”

Section: Chemical Treatment In Bovine Icsimentioning

confidence: 99%

Microtubule assembly crucial to bovine embryonic development in assisted reproductive technologies

Hochi

2016

Animal Science Journal

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Centrosome integrity and microtubule network are crucial to the events around fertilization, including pronuclear development, migration and fusion, and the first mitotic division. The present review highlights the importance of bull spermatozoal centrosomes to function as a microtubule‐organizing center for successful fertilization and the subsequent embryonic development. Spermatozoal centrosomes need to be blended with ooplasmic pericentriolar materials accurately to nucleate and organize the sperm aster. Dysfunction of the spermatozoal centrosomes is associated with fertilization failure, which has been overcome with supplemental stimuli for oocyte activation following intracytoplasmic sperm injection in humans. Even though the spermatozoal centrosomes are functionally intact, abnormal sperm aster formation was frequently observed in vitrified‐warmed bovine oocytes, with delayed pronuclear development and migration. Treatment of the post‐warm oocytes with Rho‐associated coiled‐coil kinase inhibitor or α‐tocopherol inhibited the incidence of the abnormal aster formation, resulting in higher blastocyst yields following in vitro fertilization and culture. Thus, understanding of centrosomal function made it possible to improve the performance of advanced reproductive technologies.

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“…Electostimulation of oocytes induces the ingess of calcium through formation of pores in the plasmatic membrane. This method is efficient for the activation of bovine oocytes (Hwang et al, 2000;Hosseini et al, 2008). Periodically, repeated electrostimulation imitates the fluctuation of Са 2+ , which is observed during fertilization (Ozil, 1990).…”

Section: Introductionmentioning

confidence: 99%

Influence of calcium ionophore on the fertilization of bovine oocytes and their further embryonic development

Kovpak

DERKACH

et al. 2023

Regul. Mech. Biosyst.

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Intracytoplasmic spermatozoid injection (ICSI) is one of the commonest methods used in assisted reproductive technologies in human medicine. However, this procedure has low efficacy for bovines, mainly because of insufficient activation of oocytes after spermatozoid microinjection. One of the most effective methods of activating oocytes is considered to be the use of phosphorus calcium, though the optimal concentration of activator and its effect on pre-implant development of embyo are still open questions. An oocyte-cumulus complex of clinically healthy cows, retrieved from the ovaries during slaughter, matured over 22–24 h in in vitro conditions. Oocytes with visible polar body had been subjected to intracytoplasmic spermatozoid injection (ICSI), and were 15–30 min later activated in the environment with different concentrations of calcium ionophore for 15–20 min and then transferred for further cultivation in a culture medium with sodium pyruvate. The fertilization rate was identified on the second day at the 2–4th stages of cellular embryo, and the quality of obtained embyos was evaluated on day 8. Based on the statistical analysis of the data, we determined that the artificial activation of bovine oocytes using calcium ionphore after intracytoplasmic spermatozoid injection (ICSI) led to statistically significant improvement in conception and ratio of blastocytes obtained to oocytes injected. In the study, we confirmed that addition of 5, 10 and 50 µМ of the agent had the same efficacy on the activation of occytes of bovine cattle. However, it has to be noted that during further cultivation of the obtained zygotes up to the blastocyte stage (day 8), we saw no significant differences in quality of embryos obtained. Therefore, use of calcium ionophore for the activation of bovine oocytes after intracytoplasmic spermatozoid injection is effective, for it promotes increase in fertilization parameters and ratio of blastocytes obtained to oocytes injected, facilitating production of higher numbers of embyos suitable for transplantation or cooling. Our previous conclusions are valuable for increasing the efficacy of methods of intracytoplasmic injection of bovine spermatozoid and its further use for purposes of science and production.

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Abstract: Electric stimulation before and after injection was an effective method in inducing bovine oocyte activation and in sustaining embryo development to the morulae and blastocyst stage.

Cited by 27 publications

References 17 publications

Intracytoplasmic Sperm Injection in Cattle

Intracytoplasmic Sperm Injection in Cattle

Microtubule assembly crucial to bovine embryonic development in assisted reproductive technologies

Influence of calcium ionophore on the fertilization of bovine oocytes and their further embryonic development

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