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Odee, David W.; Sutherland, J. M.; Makatiani, E. T.; McInroy, Shona G.; Sprent, Janet I.

doi:10.1023/a:1004204413140

Cited by 70 publications

(6 citation statements)

References 29 publications

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“…Fast-growing strains have been shown to be more tolerant to EST, KAN, and AMP than slow-growing strains (Odee et al, 1997). Dowdle and Bohlool (1985), however, found that slow-growing strains were more tolerant to various antibiotics.…”

Section: Antibiotic Tolerancementioning

confidence: 99%

Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species

Florentino

Sousa

Silva

et al. 2010

Rev. Bras. Ciênc. Solo

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SUMMARYThe genetic diversity of ten Bradyrhizobium strains was evaluated for tolerance to high temperatures, to different salinity levels and for the efficiency of symbiosis with cowpea plants (Vigna unguiculata (L.) Walp.). Eight of these strains were isolated from nodules that appeared on cowpea after inoculation with suspensions of soil sampled from around the root system of Sesbania virgata (wand riverhemp) in ecosystems of South Minas Gerais. The other two strains used in our analyses as references, were from the Amazon and are currently recommended as cowpea inoculants. Genetic diversity was analyzed by amplifying repetitive DNA elements with the BOX primer, revealing high genetic diversity with each strain presenting a unique band profile. Leonard jar assays showed that the strains UFLA 03-30 and UFLA 03-38 had the highest N 2 -fixing potentials in symbiosis with cowpea. These strains had more shoot and nodule dry matter, more shoot N accumulation, and a higher relative efficiency than the strains recommended as inoculants. All strains grew in media of pH levels ranging from 4.0 to 9.0. The strains with the highest N 2 -fixing efficiencies in symbiosis with cowpea were also tolerant to the greatest number of antibiotics. However, these strains also had the lowest tolerance to high salt concentrations. All strains, with the exceptions of UFLA 03-84 and UFLA 03-37, tolerated temperatures of up to 40 ºC. The genetic and phenotypic characteristics of the eight strains isolated from soils of the same region were highly variable, as well as their symbiotic efficiencies, despite their

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Section: Antibiotic Tolerancementioning

confidence: 99%

Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species

Florentino

Sousa

Silva

et al. 2010

Rev. Bras. Ciênc. Solo

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“…Although plants and animals produce amylases, enzymes from microbial sources are generally used in industrial processes. This is due to a number of factors including higher productivity, thermostability of the enzyme as well as ease of cultivating microorganisms [3] [4].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Some Amylolytic Srains of Bacteria Isolated from Peels and Soils from Two Cassava Dumpsites

Ayansina¹,

Adelaja²,

Baba³

2019

OJAppS

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Samples of peels and soils from cassava waste sites were collected from cassava processing factory in Oyo town, Oyo State and also a dumpsite in Alaya village, Aiyedire local government, Osun State, Nigeria. Bacterial isolation was carried out using the pour plate method to obtain amylolytic bacterial species capable of hydrolyzing starch. Fourteen bacterial isolates that were most responsive to starch hydrolysis were selected from both sampling sites for molecular investigations. DNA of the isolates was extracted and subjected to a cocktail mix and condition for PCR which was purified using two universal primers and afterwards the PCR product was used for the polymorphism through electrophoresis. Bacterial isolates were identified based on their genetic sequences and results showed Bacillus subtilis (21.44%) to be the most frequently occurring specie. There was the prevalence of two similar strains Bacillus subtilis MML2483 and B. subtilis MML2411 which was isolated from the different sampling sites. Some other bacterial strains included Bacillus olivae BRB18, B. licheniformis HT-26-B1, B. cereus H17, B. safensis MS40, B. pumilus 07.

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“…After purification, the cultural and biochemical characteristics of the nodule isolates were established through presumptive tests. The indigenous bradyrhizobia isolates were authenticated, confirmed as nodule-forming bacterial strains and grouped based on morphological characteristics (Odee et al, 1997). The nodule isolates were also evaluated for their ability to alkalize or acidify the media by growing them on YEMA media containing 25 mg/l of bromothymol blue.…”

Section: Morphological Characterization Of the Indigenous Nodule Isolatesmentioning

confidence: 99%

Genetic and Morphological Diversity of Indigenous Bradyrhizobium Nodulating Soybean in Organic and Conventional Family Farming Systems

Gitonga¹,

Njeru²,

Cheruiyot³

et al. 2021

Front. Sustain. Food Syst.

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Organic farming systems are gaining popularity as agronomically and environmentally sound soil management strategies with potential to enhance soil microbial diversity and fertility, environmental quality and sustainable crop production. This work aimed at understanding the effect of organic and conventional farming on the diversity of soybean nodulating bradyrhizobia species. Field trapping of indigenous soybean Bradyrhizobium was done by planting promiscuous soybeans varieties SB16 and SC squire as well as non-promiscuous Gazelle in three organic and three conventional farms in Tharaka-Nithi County of Kenya. After 45 days of growth, 108 nodule isolates were obtained from the soybean nodules and placed into 13 groups based on their morphological characteristics. Genetic diversity was done by polymerase chain reaction (PCR) targeting 16S rDNA gene using universal primers P5-R and P3-F and sequencing was carried out using the same primer. High morphological and genetic diversity of the nodule isolates was observed in organic farms as opposed to conventional farms. There was little or no genetic differentiation between the nodule isolates from the different farms with the highest molecular variation (91.12%) being partitioned within populations as opposed to among populations (8.88%). All the isolates were identified as bradyrhizobia with close evolutionary ties with Bradyrhizobium japonicum and Bradyrhizobium yuanminense. Organic farming systems favor the proliferation of bradyrhizobia species and therefore a suitable environmentally friendly alternative for enhancing soybean production.

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Cited by 70 publications

References 29 publications

Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species

Diversity and efficiency of bradyrhizobium strains isolated from soil samples collected from around sesbania virgata roots using cowpea as trap species

Molecular Characterization of Some Amylolytic Srains of Bacteria Isolated from Peels and Soils from Two Cassava Dumpsites

Genetic and Morphological Diversity of Indigenous Bradyrhizobium Nodulating Soybean in Organic and Conventional Family Farming Systems

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