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doi:10.15254/h.j.d.med.8.2016.152

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…The HPLC method was adopted from the literature with slight modifications. [ 50 ] Aliquots of CUR working solutions over the range of 50–7500 ng/mL were prepared in vials using methanol as the solvent. The concentration of CUR (ng/mL) versus area was plotted to get the calibration graph and regression equation.…”

Section: Methodsmentioning

confidence: 99%

A Sensitive Spectrofluorimetric Method for Curcumin Analysis

et al. 2022

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Curcumin (CUR), a natural polyphenolic compound extracted from the rhizomes of Curcuma longa, is used as a pharmaceutical agent, spice in food, and as a dye. Currently, CUR is being investigated for cancer treatment in Phase-II clinical trials. CUR also possesses excellent activities like anti-inflammatory, anti-microbial, and anti-oxidant, therefore quality control is crucial. The present research work was to develop a new, simple, validated and time-saving rapid 96-well plate spectrofluorimetric method for the determination of CUR. The developed method was compared with routinely used high performance liquid chromatography (HPLC) technique. The developed method were found to be linear in the concentration range of 15 to 3900 ng/mL with R2 ≥ 0.9983 for spectrofluorimetric and 50-7500 ng/mL with R2 ≥ 0.9999 for HPLC method. Accuracy, intraday and interday precision was adequate, with RSD lower than the suggested limits. The limits for the detection and the quantification of CUR were 7 and 15 ng/mL for spectrofluorimetric, and 25 and 50 ng/mL for HPLC respectively. The Bland-Altman analysis demonstrated the similarities between the two methods. The 96-well plate method was successfully applied to determine CUR in solid lipid nanoparticles (SLNs) and chitosan nanoparticles (Chi-NPs). The developed spectrofluorimetric method can hence serve as a possible replacement for the HPLC method for the quantification of CUR in healthcare and food products.

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Section: Methodsmentioning

confidence: 99%

A Sensitive Spectrofluorimetric Method for Curcumin Analysis

et al. 2022

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A robust RP-HPLC method for determination of turmeric adulteration

Sahu¹,

Panda²,

Kumar³

et al. 2020

Journal of Liquid Chromatography & Related Technologies

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Curcumin: Biological Activities and Modern Pharmaceutical Forms

et al. 2022

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Curcumin (1,7-bis-(4-hydroxy-3-methoxyphenyl)-hepta-1,6-diene-3,5-dione) is a natural lipophilic polyphenol that exhibits significant pharmacological effects in vitro and in vivo through various mechanisms of action. Numerous studies have identified and characterised the pharmacokinetic, pharmacodynamic, and clinical properties of curcumin. Curcumin has an anti-inflammatory, antioxidative, antinociceptive, antiparasitic, antimalarial effect, and it is used as a wound-healing agent. However, poor curcumin absorption in the small intestine, fast metabolism, and fast systemic elimination cause poor bioavailability of curcumin in human beings. In order to overcome these problems, a number of curcumin formulations have been developed. The aim of this paper is to provide an overview of recent research in biological and pharmaceutical aspects of curcumin, methods of sample preparation for its isolation (Soxhlet extraction, ultrasound extraction, pressurised fluid extraction, microwave extraction, enzyme-assisted aided extraction), analytical methods (FTIR, NIR, FT-Raman, UV-VIS, NMR, XRD, DSC, TLC, HPLC, HPTLC, LC-MS, UPLC/Q-TOF-MS) for identification and quantification of curcumin in different matrices, and different techniques for developing formulations. The optimal sample preparation and use of an appropriate analytical method will significantly improve the evaluation of formulations and the biological activity of curcumin.

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Abstract: Plan: Validation of a method for the HPLC estimation of curcuminoids

Cited by 3 publications

References 15 publications

A Sensitive Spectrofluorimetric Method for Curcumin Analysis

A Sensitive Spectrofluorimetric Method for Curcumin Analysis

A robust RP-HPLC method for determination of turmeric adulteration

Curcumin: Biological Activities and Modern Pharmaceutical Forms

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