A Sensitive Spectrofluorimetric Method for Curcumin Analysis

Abstract

Curcumin (CUR), a natural polyphenolic compound extracted from the rhizomes of Curcuma longa, is used as a pharmaceutical agent, spice in food, and as a dye. Currently, CUR is being investigated for cancer treatment in Phase-II clinical trials. CUR also possesses excellent activities like anti-inflammatory, anti-microbial, and anti-oxidant, therefore quality control is crucial. The present research work was to develop a new, simple, validated and time-saving rapid 96-well plate spectrofluorimetric method for the determination of CUR. The developed method was compared with routinely used high performance liquid chromatography (HPLC) technique. The developed method were found to be linear in the concentration range of 15 to 3900 ng/mL with R2 ≥ 0.9983 for spectrofluorimetric and 50-7500 ng/mL with R2 ≥ 0.9999 for HPLC method. Accuracy, intraday and interday precision was adequate, with RSD lower than the suggested limits. The limits for the detection and the quantification of CUR were 7 and 15 ng/mL for spectrofluorimetric, and 25 and 50 ng/mL for HPLC respectively. The Bland-Altman analysis demonstrated the similarities between the two methods. The 96-well plate method was successfully applied to determine CUR in solid lipid nanoparticles (SLNs) and chitosan nanoparticles (Chi-NPs). The developed spectrofluorimetric method can hence serve as a possible replacement for the HPLC method for the quantification of CUR in healthcare and food products.

Topical delivery of extracted curcumin as curcumin loaded spanlastics anti-aging gel: Optimization using experimental design and ex-vivo evaluation

“…Spectrophotometric method provides an inexpensive, rapid, specific, sensitive, precise, reliable and accurate method for the analysis of Curcumin ( Kadam et al, 2018 , Sravani et al, 2022 ).…”

Curcumin Electrochemistry—Antioxidant Activity Assessment, Voltammetric Behavior and Quantitative Determination, Applications as Electrode Modifier

“…Among the CU pro-health activities are also the antibac-terial effect discovered in 1949 [12,15], antiviral (against HIV, HPV, hepatitis virus, etc.) [57], and immunomodulatory and potentially antiallergic ones [1].…”

“…Among the CU pro-health activities are also the antibacterial effect discovered in 1949 [12,15], antiviral (against HIV, HPV, hepatitis virus, etc.) [57], and immunomodulatory and potentially antiallergic ones [1]. Despite its many health benefits, which were also discussed in different sections of some reviews [10,12,58,59], CU therapeutic use is limited by its reduced bioavailability generated by its low aqueous solubility, poor intestinal absorption, rapid metabolism and excretion from the body, 75% of the administrated CU dose being found in animal feces [10].…”

Liquid crystal precursor system as a vehicle for curcumin‐mediated photodynamic inactivation of oral biofilms

“…The study of C-LCP release was performed according to a previously published protocol [52][53][54] with some modifications. Briefly, a Microette equipment consisting of Franz diffusion cells (Hanson Research, Chatsworth, GA, USA) was used with a polyethersulfone synthetic membrane (Ht Tuffryn, 25 mm diameter and 0.45 μm pore size; Pall Life Sciences, New York, NY, USA) and a receptor solution comprising phosphate buffer (pH 6.8) with 15% propylene glycol, 1% Span 60 (Sigma-Aldrich, San Luis, MO, EUA), and 5% ethanol at 37 ± 0.5 C stirred at 300 rpm.…”