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Voronov, Sergei; Binevski, Petr V.; Eremin, Sergei A.; Кост, О. А.

doi:10.1023/a:1010220930765

Cited by 8 publications

(7 citation statements)

References 24 publications

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“…The existence of a ‘silent’ active site is highly unlikely as bovine ACE is highly homologous to the human enzyme, which has two functional active sites. Moreover, in a previous fluorescence polarization study [34] of different bovine ACE forms we showed the presence of two populations of complexes between somatic enzyme and fluorescent‐labeled lisinopril in conditions of a significant lack of the inhibitor; that was attributed to the formation of complexes of labeled lisinopril with different ACE domains.…”

Section: Discussionmentioning

confidence: 69%

Evidence for the negative cooperativity of the two active sites within bovine somatic angiotensin‐converting enzyme

et al. 2003

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The somatic isoform of angiotensin-converting enzyme (ACE) consists of two homologous domains (N-and C-domains), each bearing a catalytic site. We have used the twodomain ACE form and its individual domains to compare characteristics of di¡erent domains and to probe mutual functioning of the two active sites within a bovine ACE molecule. The substrate Cbz-Phe-His-Leu (N-carbobenzoxy-L-phenylalanyl-L-histidyl-L-leucine; from the panel of seven) was hydrolyzed faster by the N-domain, the substrates FA-Phe-Gly-Gly (N-(3-[2-furyl]acryloyl)-L-phenylalanyl-glycyl-glycine) and Hip-His-Leu (N-benzoyl-glycyl-L-histidyl-L-leucine) were hydrolyzed by both domains with equal rates, while other substrates were preferentially hydrolyzed by the C-domain. The inhibitor captopril ((2S)-1-(3-mercapto-2-methylpropionyl)-L-proline) bound to the N-domain more e¡ectively than to the C-domain, whereas lisinopril ((S)-N K -(1-carboxy-3-phenylpropyl)-L-lysyl-L-proline) bound to equal extent with all ACE forms. However, active site titration with lisinopril assayed by hydrolysis of FA-PheGly-Gly revealed that 1 mol of inhibitor/mol of enzyme abolished the activity of either two-domain or single-domain ACE forms, indicating that a single active site functions in bovine somatic ACE. Neither of the k cat values obtained for somatic enzyme was the sum of k cat values for individual domains, but in every case the value of the catalytic constant of the hydrolysis of the substrate by the two-domain ACE represented the mean quantity of the values of the corresponding catalytic constants obtained for single-domain forms. The results indicate that the two active sites within bovine somatic ACE exhibit strong negative cooperativity. ß

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Section: Discussionmentioning

confidence: 69%

Evidence for the negative cooperativity of the two active sites within bovine somatic angiotensin‐converting enzyme

et al. 2003

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“…In order to identify the features of the initial stages of the formation of vegetation cover on the dump, experimental sites were formed through different combinations of FSL and PFR: option I -application of FSL with a thickness of 0.5 m; option II -control, material of the dump; option IIIapplication of PFR with a thickness of 1 m; option IV -layer-by-layer application of PFR with a thickness of 0.6 m and FSL with a thickness of 0.4 m. [4]. At each site, a series of geobotanical descriptions were carried out (accounting plots with an area of 100 m2) and, the gathering of aboveground phytomass (mowing) was carried out in three iterations on plots with an area of 1 m 2 [5].…”

Section: Methodsmentioning

confidence: 99%

Restoration of plant communities on various dump substrates

Gossen

Solovyev

2021

BIO Web Conf.

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The results of field studies in 2018 and 2019 at the pilot production site on the territory of the external dump “Zarechny” of JSC SUEK-Kuzbass are presented. The ecological and coenotic characteristics of plant communities formed in the areas with the filling of the fertile soil layer (FSL) (0.5 m thickness), with the application of potentially fertile rocks (PFR) (1 m thickness), in the area with the filling of technogenic eluvium, as well as in the area with the layer-by-layer filling of PFR (0.6 m thickness) and FSL (0.4 m thickness) were studied. Geobotanical descriptions were carried out according to standard methods, taking into account iterations. It is established that in the first years after the end of the formation of the pilot production site, the number of herbaceous plant species increases in all areas. However, the use of lithogenic resources in the frame of reclamation accelerates the development of plant communities on man-made landscapes, but does not allow reaching the stages of zonal formations.

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“…Field work was carried out in August 2020. On each trial plot, geobotanical descriptions were carried out according to the standard technique [ 23 ]: the species composition of plants for the herb-dwarf shrub and the moss-lichen aboveground layers, and the projective cover and the height of the tiers were taken into account. Vascular plant species were assigned to seven functional groups: tall shrubs ( Duschekia fruticosa (Rupr.)…”

Section: Methodsmentioning

confidence: 99%

Relationship between Species Richness, Biomass and Structure of Vegetation and Mycobiota along an Altitudinal Transect in the Polar Urals

Shiryaev

Peintner

Elsakov

et al. 2020

JoF

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Aboveground species richness patterns of vascular plants, aphyllophoroid macrofungi, bryophytes and lichens were compared along an altitudinal gradient (80–310 m a.s.l.) on the Slantsevaya mountain at the eastern macroslope of the Polar Urals (Russia). Five altitudinal levels were included in the study: (1) Northern boreal forest with larch-spruce in the Sob’ river valley habitats; (2–3) two levels of closed, northern boreal, larch-dominated forests on the slopes; (4) crook-stemmed forest; (5) tundra habitats above the timberline. Vascular plant or bryophyte species richness was not affected by altitudinal levels, but lichen species richness significantly increased from the river valley to the tundra. For aphyllophoroid macrofungi, species richness was highest at intermediate and low altitudes, and poorest in the tundra. These results indicate a positive ecotone effect on aphyllophoroid fungal species richness. The species richness of aphyllophoroid fungi as a whole was neither correlated to mortmass stocks, nor to species richness of vascular plants, but individual ecological or morphological groups depended on these parameters. Poroid fungal species richness was positively correlated to tree age, wood biomass and crown density, and therefore peaked in the middle of the slope and at the foot of the mountain. In contrast, clavarioid fungal species richness was negatively related to woody bio- and mortmass, and therefore peaked in the tundra. This altitudinal level was characterized by high biomass proportions of lichens and mosses, and by high litter mortmass. The proportion of corticoid fungi increased with altitude, reaching its maximum at the timberline. Results from the different methods used in this work were concordant, and showed significant patterns. Tundra communities differ significantly from the forest communities, as is also confirmed by nonmetric multidimensional scaling (NMDS) analyses based on the spectrum of morphological and ecological groups of aphyllophoroid fungi.

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Untitled

Cited by 8 publications

References 24 publications

Evidence for the negative cooperativity of the two active sites within bovine somatic angiotensin‐converting enzyme

Evidence for the negative cooperativity of the two active sites within bovine somatic angiotensin‐converting enzyme

Restoration of plant communities on various dump substrates

Relationship between Species Richness, Biomass and Structure of Vegetation and Mycobiota along an Altitudinal Transect in the Polar Urals

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