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Høgset, Anders; Prasmickaite, Lina; Hellum, Marit; Engesæter, Birgit; Olsen, Vibeke Murberg; Tjelle, Torunn Elisabeth; Wheeler, Carl J.; Berg, Kristian

doi:10.1023/a:1022979806314

Cited by 31 publications

(10 citation statements)

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“…Photochemical internalisation has been studied as a gene delivery technology (reviewed in [27]) both with several non-viral [28][29][30] and with adenoviral vectors [31] as well as adeno-associated virus [32], mainly by using reporter genes such as genes encoding EGFP (enhanced green fluorescent protein) or ß-galactosidase. Agostin [15] Horseradish peroxidase TPPS2a [15] Targeted Proteins…”

Section: Pci For Gene Deliverymentioning

confidence: 99%

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Photochemical Internalization: A New Tool for Drug Delivery

Berg

Folini

Prasmickaite³

et al. 2007

CPB

Self Cite

124

100

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The utilisation of macromolecules in the therapy of cancer and other diseases is becoming increasingly important. Recent advances in molecular biology and biotechnology have made it possible to improve targeting and design of cytotoxic agents, DNA complexes and other macromolecules for clinical applications. In many cases the targets of macromolecular therapeutics are intracellular. However, degradation of macromolecules in endocytic vesicles after uptake by endocytosis is a major intracellular barrier for the therapeutic application of macromolecules having intracellular targets of action. Photochemical internalisation (PCI) is a novel technology for the release of endocytosed macromolecules into the cytosol. The technology is based on the activation by light of photosensitizers located in endocytic vesicles to induce the release of macromolecules from the endocytic vesicles. Thereby, endocytosed molecules can be released to reach their target of action before being degraded in lysosomes. PCI has been shown to stimulate intracellular delivery of a large variety of macromolecules and other molecules that do not readily penetrate the plasma membrane, including type I ribosome-inactivating proteins (RIPs), DNA delivered as gene-encoding plasmids or by means of adenovirus or adeno-associated virus, peptide nucleic acids (PNAs) and chemotherapeutic agents such as bleomycin and in some cases doxorubicin. PCI of PNA may be of particular importance due to the low therapeutic efficacy of PNA in the absence of an efficient delivery technology and the 10-100-fold increased efficacy in combination with PCI. The efficacy and specificity of PCI of macromolecular therapeutics has been improved by combining the macromolecules with targeting moieties, such as the epidermal growth factor. In general, PCI can induce efficient light-directed delivery of macromolecules into the cytosol, indicating that it may have a variety of useful applications for site-specific drug delivery as for example in gene therapy, vaccination and cancer treatment.

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Section: Pci For Gene Deliverymentioning

confidence: 99%

“…AdCMV-EGFP TPPS2a [32,83] AdCMV-lacZ AlPcS2a , TPPS2a [83][84][85] AdRGD-EGFP TPPS2a [47] AdSV40-EGFP TPPS2a [48] AdvCMV-TRAIL TPPS2a [86] AAV5RSV-EGFP TPPS2a [32] Non-Virus Mediated:…”

Section: Genes (Reporters and Therapeutic-relevant)mentioning

confidence: 99%

Photochemical Internalization: A New Tool for Drug Delivery

Berg

Folini

Prasmickaite³

et al. 2007

CPB

Self Cite

124

100

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“…[10][11][12] Such treatment strategy mainly based on that P-gp inhibition was not associated with the PDT, and ROS produced in the process of PDT could not only directly kill tumor cells but also induce photooxidation of endocytic membranes and finally lead to release of chemotherapeutics from endosomes to cytosol. [13][14][15] To obtain such synergistic effect, one of the widely-used methods is to deliver the photosensitizer and chemotherapeutics using a single nanosystem.…”

Section: Introductionmentioning

confidence: 99%

Tumor-Homing and Penetrating Peptide-Functionalized Photosensitizer-Conjugated PEG-PLA Nanoparticles for Chemo-Photodynamic Combination Therapy of Drug-Resistant Cancer

Feng

Jiang

Kang

et al. 2016

ACS Appl. Mater. Interfaces

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The combination of photodynamic therapy (PDT) and chemotherapy holds great potential in combating drug-resistant cancers. However, the major challenge that lies ahead is how to achieve high coloading capacity for both photosensitizer and chemo-drugs and how to gain efficient delivery of drugs to the drug-resistant tumors. In this study, we prepared a nanovehicle for codelivery of photosensitizer (pyropheophorbide-a, PPa) and chemo-drugs (paclitaxel, PTX) based on the synthesis of PPa-conjugated amphiphilic copolymer PPa-PLA-PEG-PLA-PPa. The obtained nanoparticles (PP NP) exhibited a satisfactory high drug-loading capacity for both drugs. To achieve effective tumor-targeting therapy, the surface of PP NP was decorated with a tumor-homing and penetrating peptide F3. In vitro cellular experiments showed that F3-functionalized PP NP (F3-PP NP) exhibited higher cellular association than PP NP and resulted in the strongest antiproliferation effect. In addition, compared with the unmodified nanoparticles, F3-PP NP exhibited a more preferential enrichment at the tumor site. Pharmacodynamics evaluation in vivo demonstrated that a longer survival time was achieved by the tumor-bearing mice treated with PP NP (+laser) than those treated with chemotherapy only or PDT only. Such antitumor efficacy of combination therapy was further improved following the F3 peptide functionalization. Collectively, these results suggested that targeted combination therapy may pave a promising way for the therapy of drug-resistant tumor.

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“…[277] Another approach to overcome the endosomal membrane called "photochemical internalization" (PCI) has been introduced by Berg et al [278] based upon light activation of a photosensitizer which is specifically located at the membrane of endocytic vesicles which allows the endosomal escape of the polyplexes in a light-inducible manner. [279][280][281][282][283] This novel technology makes use of the generation of singlet oxygen by excitation of a photosensitizer, usually porphyrins or porphyrin-related compounds (Figure 20), which is added together with the non-viral vector to the cell together.…”

Section: Introductionmentioning

confidence: 99%

“…[279] Singlet oxygen is a highly reactive species which is known to oxidize membrane lipids, proteins, amino acids and several other organic macromolecules close to the site of generation. [285] In the past decade several approaches to deliver DNA/ siRNA into the cell have been developed.…”

Section: Introductionmentioning

confidence: 99%