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Abstract

A cDNA coding for a putative organic cation transporter (OCT) was isolated from Phaseolus vulgaris roots by differential display RT-PCR and the corresponding full-length cDNA (named PvOCT1) was subsequently obtained by RACE-PCR. Hydropathy profiles of the deduced amino acid sequence (547 residues) predicted the existence of twelve membrane-spanning domains, which are highly conserved in the major facilitator superfamily (MFS). Three specific domains, which characterize organic ion transporters in animals, can also be observed in the predicted protein. In the non-stressed plants, northern analysis showed that PvOCT1 is strongly expressed in roots and stems, while in situ hybridization revealed the presence of PvOCT1 transcripts in phloem cells. In roots PvOCT1 transcript levels transitorily increased after one hour of dehydration and then dramatically decreased. This decrease was associated with enhanced abundance of PvNeED1 mRNA encoding the enzyme thought to catalyze the limiting step of abscisic acid biosynthesis.

Root System Architecture and Omics Approaches for Belowground Abiotic Stress Tolerance in Plants

“…Under water-scarce conditions, novel dehydration responsive genes were acknowledged in roots of common bean through differential display RT-PCR analysis. Further studies demonstrated that a dehydration-responsive gene, OCT1, gets upregulated in the roots of common bean after 1 h of dehydration and further gets depleted after de novo synthesis of ABA [74]. Differential regulation of aquaporin and vacuolar-specific transcripts were observed under drought stress in Cicer arientum L. [46].…”

Understanding role of roots in plant response to drought: Way forward to climate‐resilient crops

“…High‐throughput transcriptome studies have been conducted on plants subjected to drought stress, which highlighted the role of NAC‐encoding genes that are expressed in roots (Janiak et al., 2016); for instance, rice plants, overexpressing OsNAC10, had more thicker roots with enlarged epidermis and cortex (Jeong et al., 2010). Differential display RT‐PCR analysis in common beans showed many unique dehydration‐responsive genes that are expressed in roots under drought stress (Torres et al., 2003). In chickpeas, upregulation of various stress‐responsive genes such as root nodulation genes, genes involved in biosynthesis of oxylipin, and various TFs has been observed in root transcriptome profile during water scarcity (Bhaskarla et al., 2020).…”