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Куперман, Б. И.; Matey, Victoria E.; Hurlbert, Stuart H.

doi:10.1023/a:1014555904968

Cited by 30 publications

(3 citation statements)

References 37 publications

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“…The skin, even if abundantly infested by trophonts, did not show clear lesions nor the typical dusty appearance as described in other species, and from which derives the common name of the disease (marine velvet disease). Ultimately, the observed histological findings are consistent with those reported in previous studies conducted on other fish species naturally infested by A. ocellatum [2,4,5,7,8,13,18] and in A. ocellatum infested ESB [10]. In general, the inflammatory cell infiltrates were scarce in the gills, in agreement with the description provided by Paperna [7] on gilthead sea bream (Sparus aurata).…”

Section: Discussionsupporting

confidence: 91%

“…Ultrastructural studies have better clarified the morphology of the parasite and the stomopode conformation/composition, including aspects of parasite-host interaction with three cyprinodontid species [12]. Scanning electron microscopy was used to detail local erosion and the distortion of the gill epithelium at the site of trophont attachment on naturally infested fish living in the Salton Sea Lake [2,13].…”

Section: Introductionmentioning

confidence: 99%

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News Insights into the Host-Parasite Interactions of Amyloodiniosis in European Sea Bass: A Multi-Modal Approach

et al. 2022

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Amyloodiniosis is a disease resulting from infestation by the ectoparasitic dinoflagellate Amyloodinium ocellatum (AO) and is a threat for fish species such as European sea bass (ESB, Dicentrarchus labrax), which are farmed in lagoon and land-based rearing sites. During the summer, when temperatures are highest, mortality rates can reach 100%, with serious impacts for the aquaculture industry. As no effective licensed therapies currently exist, this study was undertaken to improve knowledge of the biology of AO and of the host-parasite relationship between the protozoan and ESB, in order to formulate better prophylactic/therapeutic treatments targeting AO. To achieve this, a multi-modal study was performed involving a broad range of analytical modalities, including conventional histology (HIS), immunohistochemistry (IHC) and confocal laser scanning microscopy (CLSM). Gills and the oro-pharyngeal cavity were the primary sites of amyloodiniosis, with hyperplasia and cell degeneration more evident in severe infestations (HIS). Plasmacells and macrophages were localised by IHC and correlated with the parasite burden in a time-course experimental challenge. CLSM allowed reconstruction of the 3D morphology of infecting trophonts and suggested a protein composition for its anchoring and feeding structures. These findings provide a potential starting point for the development of new prophylactic/therapeutic controls.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

News Insights into the Host-Parasite Interactions of Amyloodiniosis in European Sea Bass: A Multi-Modal Approach

et al. 2022

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“…In addition to the stressor of interest (MS-222), handling the fish and surgery itself can induce osmotic imbalance. Handling fish can affect the fish's skin permeability and thus its osmotic homeostasis (Kuperman et al 2001;Zydlewski et al 2010). This likely occurs during invasive surgeries were the skin is cut in order to intra-coelomically implant acoustic tags.…”

Section: 3mentioning

confidence: 99%

Determine the Influence of Time Held in ?Knockdown? Anesthesia on Survival and Stress of Surgically Implanted Juvenile Salmonids

Woodley¹,

Wagner²,

Knox³

2012

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SummaryThis report summarizes the responses of juvenile Chinook salmon (Oncorhynchus tshawytscha) subjected to the anesthetic, tricaine methanesulfonate (MS-222), during surgical implantation of acoustic transmitters (ATs) and passive integrated transponders (PITs). The Columbia Basin Surgical Protocol Steering Committee expressed concern that the recommendation of a 10-minute maximum exposure to MS-222 could be too long for a juvenile salmonid resulting in increased behavioral and physiological costs, and/or decreased survival. These undesirable results are further problematic because survival study assumptions require that fish are representative of fish in the river that have not been subjected to handling, holding, and surgical implantation. Thus, there is a need to determine the amount of time fish can be held in knockdown anesthetic without negatively influencing their survival and thus fish passage survival models. The study reported here examined the relationship between stress indicators for several blood analytes and biochemistries (sodium, calcium, potassium, blood pH, plasma cortisol), time under anesthesia, and number of days post-exposure to anesthesia in juvenile yearling Chinook salmon (YCH). Fish were randomly assigned to one of three treatment groups: anesthetic control (no anesthetic exposure, no surgical implantation)  surgical control (exposed to anesthesia and removed when reached Stage 4 anesthesia, no surgical implantation) one of seven treatments where fish remained in the anesthetic for 0, 3, 5, 6, 9, 12, or 15 minutes beyond the "knockdown" time (KD) when they reached Stage 4 anesthesia and were surgically implanted with an AT and PIT.Controls and KD treatment exposure groups were further assigned to one of four post-surgical implantation-monitoring days: 0, 1, 7, or 14 days after treatment. Day 0 fish were immediately sampled after the anesthetic treatment; while 1, 7, and 14 day fish were held until their respective day for sampling and termination. The purpose of the study was to address three questions:1. Did the MS-222 KD treatment exposure groups result in differential mortality for surgically implanted fish?2. Did the stress responses of juvenile Chinook salmon vary significantly as a result of KD on day 0 and when compared to the anesthetic control fish on day 0?3. Did the stress responses of juvenile Chinook salmon vary significantly as a result of the KD when examined on post-surgical implantation-monitoring days 1, 7, 14; and when compared to surgical control fish over the same time periods?Although we expected to see mortalities in the 12-and 15-minute extended KD treatment exposures, there were no mortalities in any of the controls or KD exposure times. This suggests that perhaps: 1) the duration of exposure (KD), at 80 mg/L, in anesthetic prior to surgical implantation does not directly affect fish survival over the time range tested; 2) the life stage of holdover juvenile YCH may be less susceptible to anesthetic stress and thus mortality than actively outmigrating ju...

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