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Tao, Jun; Tamis, Robert; Fink, Katharine

doi:10.1023/a:1009464114488

Cited by 6 publications

(1 citation statement)

References 21 publications

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“…This indicates that in the presence of hypertonic cryoprotectants, AQP3 seems to be the main media of fluid exchange, stimulated to undergo protein localization and enhance expression. In this way, embryo is able to endure changes in osmotic pressure during cryopreservation and thawing since water and cryoprotectants can flow into and out of blastomeres effectively [18]. Besides, we speculate that other AQP subtypes may play a more dominant role in the transport and distribution of fluids during freeze–thawing of blastocyst stage and 8-cell stage.…”

Section: Discussionmentioning

confidence: 99%

The expression and distribution of aquaporin 3 in mouse embryos before and after vitrification

Nong

Chen

et al. 2013

J Assist Reprod Genet

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PurposeTo determine the role of aquaporin 3 (AQP3) isoform in early embryonic development and protecting embryos subjected to freeze–thawing for assisted reproduction, we investigated the expression and distribution of AQP3 in mouse embryos at different developmental stages before and after vitrification.MethodsEight-cell embryos, morulae, and blastocysts were obtained from female mice that had been superovulated by controlled ovarian hyperstimulation. Immunofluorescence staining, laser confocal microscopy, and Western blot were used to determine the expression and distribution of AQP3 in preimplantation mouse embryos before and after vitrification.ResultsAQP3 was expressed at the 8-cell to blastocyst stage before and after vitrification. The expression and distribution of AQP3 was developmentally regulated at the 8-cell to blastocyst stage. The expression of AQP3 was significantly decreased in 8-cell embryos and early blastocysts after vitrification. However, at the morulae stage, the expression of AQP3 was increased after vitrification.ConclusionsThe developmental and vitrification-dependent changes in AQP3 expression and distribution suggest that this transmembrane channel might regulate mouse embryo development and contribute to the protective response during vitrification.

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Section: Discussionmentioning

confidence: 99%