1990
DOI: 10.1063/1.1141360
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A 10-GHz frequency-domain fluorometer

Abstract: We describe the design and performance of a 10-GHz harmonic-content frequency-domain fluorometer. The modulated excitation is provided by the harmonic content of a train of ps pulses. High-speed and/or high-frequency detection was attained with a triode-type microchannel plate photomultiplier tube (MCP PMT) from Hamamatsu, R-2566-6, with 6 μm channels. To minimize the cost of the electronic components, and to minimize the noise due to these components, the detection circuits consists of two frequency ranges, 1… Show more

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Cited by 208 publications
(151 citation statements)
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“…The values of α i and τ i were determined by nonlinear least squares impulse reconvolution with a goodness-of-fit criterion [26].…”
Section: Discussionmentioning
confidence: 99%
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“…The values of α i and τ i were determined by nonlinear least squares impulse reconvolution with a goodness-of-fit criterion [26].…”
Section: Discussionmentioning
confidence: 99%
“…Gold colloids were prepared by the citrate reduction of HAuCL 4 [25][26][27]. Some 68 mg HAuCL 4 was dissolved in 200 ml water (1 mM) and brought to the boil with vigorous stirring, followed by the addition of 20 ml (38.8 mM) sodium citrate solution.…”
Section: Experimental Materialsmentioning
confidence: 99%
“…The FD intensity decay were measured using 10 GHz fluorometer [28] and analyzed in terms of the multi-exponential model (1) where τ i are the lifetimes with amplitudes α i and Σ α i = 1.0. The contribution of each component to the steady state intensity is given by (2) where the sum in the denominator is over all the decay times and amplitudes.…”
Section: Fluorescence Measurementsmentioning
confidence: 99%
“…14 Based on the analogy between SPR and SPCE, we expect fluorophore coupling to the metal to also depend on the depth of the evanescent field. For SPR, the electrical field decays exponentially as (9) where E 0 is the field strength at z = 0 and k z −1 is the penetration depth into the sample. The values of k z can be calculated using (10) where ω is the frequency of the incident light.…”
Section: Fluorescence Measurementsmentioning
confidence: 99%
“…The output of fiber was directed to 8000 SLM spectrofluorometer or 10-GHz frequency domain fluorometer. 9 For excitation, we used the second harmonic (300 nm) from a Rhodamine 6G dye laser. Dye laser was pumped by a mode-locked argon ion laser, 76-MHz repetition rate, 120-ps half-width, and cavity dumped at 3.8-MHz repetition rate.…”
Section: Fluorescence Measurementsmentioning
confidence: 99%