A 20-kDa pilus protein with haemagglutination and intestinal adherence properties expressed by a clinical isolate of non-01 Vibrio cholerae

Sengupta, T

doi:10.1016/0378-1097(93)90170-7

Cited by 5 publications

(24 citation statements)

References 16 publications

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“…The non-O1/non-O139 V. cholerae strain (10325) belonging to serovar O34 was isolated from the stool of a patient with diarrhea [13,18]. Other V. cholerae strains used in this study were described earlier [13].…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…Harvested bacteria were disintegrated by an ultrasonic disintegrator (Labsonic, B. Braun) and the crude outer membrane (OM) material containing the pilus protein was collected [18]. This material was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and the electrophoresed gel was treated with 1 M KCl to visualize the protein bands.…”

Section: Isolation and Amino-terminal Sequencementioning

confidence: 99%

“…Methods for the preparation of the IgG fraction of the antiserum and IgG (Fab) fragments were described earlier [18].…”

Section: Raising Of Antiserum and Preparation Of Fab (Igg) Fragmentmentioning

confidence: 99%

“…An antiserum to the 20-kDa protein of strain 10325 was raised in rabbits through immunization with the material containing the desired protein cut from the SDS gel [18]. Methods for the preparation of the IgG fraction of the antiserum and IgG (Fab) fragments were described earlier [18].…”

Section: Raising Of Antiserum and Preparation Of Fab (Igg) Fragmentmentioning

confidence: 99%

“…In an earlier report [18], we described a new type of pilus expressed by a diarrheogenic non-O1/non-O139 strain (10325) of V. cholerae. The pilus, which is morphologically as well as antigenically distinct from TCP and other types of pili expressed by V. cholerae, was shown to be composed of 20-kDa subunit proteins.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a 20-kDa pilus protein expressed by a diarrheogenic strain of non-O1/non-O139 Vibrio cholerae

Sengupta

1998

FEMS Microbiology Letters

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A diarrheogenic strain of non-O1/non-O139 Vibrio cholerae (10325) belonging to serogroup O34 was earlier shown to express a new type of pilus composed of a 20-kDa subunit protein. Amino-terminal sequence data (determined up to 20 amino acid residues) of this protein showed it to be different from the subunit proteins of other known types of pili of V. cholerae. On the other hand, it showed complete homology with the corresponding sequence of a 22-kDa outer membrane protein (OmpW) of V. cholerae. Expression of 10325 pili was favored in AKI rather than in NB medium and at 30³C rather than at 37³C. Further, cultural conditions favoring pilus expression also enhanced autoagglutination and adherence properties of strain 10325. An antiserum to the 20-kDa protein induced passive protection against challenge with the parent organism 10325, but not against V. cholerae O1 strains. Such protection was shown to be mediated by inhibition of intestinal colonization in vivo. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

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Section: Bacterial Strainsmentioning

confidence: 99%

Section: Isolation and Amino-terminal Sequencementioning

confidence: 99%

“…Methods for the preparation of the IgG fraction of the antiserum and IgG (Fab) fragments were described earlier [18].…”

Section: Raising Of Antiserum and Preparation Of Fab (Igg) Fragmentmentioning

confidence: 99%

Section: Raising Of Antiserum and Preparation Of Fab (Igg) Fragmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of a 20-kDa pilus protein expressed by a diarrheogenic strain of non-O1/non-O139 Vibrio cholerae

Sengupta

1998

FEMS Microbiology Letters

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Epidemic isolates of Vibrio cholerae 0139 express antigenically distinct types of colonization pili

Sengupta

1994

FEMS Microbiology Letters

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Vibrio cholerae belonging to the recently described serogroup 0139, which are responsible for the current cholera epidemics in India and Bangladesh, were shown to express pilus-like structures partially cross-reacting with the toxin-coregulated pilus of V. cholerae strain (0395) belonging to the 01 serogroup and classical biotype. The 0139 pili were composed of 20 kDa subunit proteins which were antigenically related to the 20 kDa pilus protein of another diarrhoeagenic non-01 V. cholerae strain (serogroup 034) isolated earlier. The pili described in this study were found to be involved in the intestinal colonization process and, therefore, may contribute towards the virulence of the 0139 epidemic isolates.

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Expression of antigenically distinct fimbriae with hemagglutination and HeLa cell adherence properties by an enteroaggregative Escherichia coli strain belonging to the enteropathogenic serogroup

Nandy¹

1994

FEMS Immunology and Medical Microbiology

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An enteroaggregative Escherichia coli (EAggEC) strain (DS92), isolated from a case of infantile diarrhea, was shown to express mannose-resistant hemagglutination and HeLa cell adhering properties when grown at 37 degrees C but not at 28 degrees C. Cellular adherence properties of DS92, which belonged to enteropathogenic serogroup 0125, were shown to correlate well with the expression of fimbriae that were encoded by a 112 kb plasmid. The fimbriae of the EAggEC strain DS92 were composed of 20 kDa subunit proteins and were serologically distinct from fimbrial or non-fimbrial cell surface antigen(s) of other diarrheagenic E. coli strains including the reference EAggEC strain 17-2. Interestingly, the 20-kDa fimbrial protein was found to be antigenically related to 18- and 14.5-kDa cell surface proteins of two other locally isolated EAggEC strains belonging to the enteropathogenic serogroup 086.

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A 20-kDa pilus protein with haemagglutination and intestinal adherence properties expressed by a clinical isolate of non-01 Vibrio cholerae

Cited by 5 publications

References 16 publications

Characterization of a 20-kDa pilus protein expressed by a diarrheogenic strain of non-O1/non-O139 Vibrio cholerae

Characterization of a 20-kDa pilus protein expressed by a diarrheogenic strain of non-O1/non-O139 Vibrio cholerae

Epidemic isolates of Vibrio cholerae 0139 express antigenically distinct types of colonization pili

Expression of antigenically distinct fimbriae with hemagglutination and HeLa cell adherence properties by an enteroaggregative Escherichia coli strain belonging to the enteropathogenic serogroup

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