A 3-year multi-food study of the presence and persistence of Listeria monocytogenes in 54 small food businesses in Ireland

Leong, Dara; NicAogáin, Kerrie; Luque-Sastre, Laura; McManamon, Oisin; Hunt, Karen A.; Álvarez‐Ordóñez, Avelino; Scollard, Johann; Schmalenberger, Achim; Fanning, Séamus; O’Byrne, Conor; Jordan, Kieran

doi:10.1016/j.ijfoodmicro.2017.02.015

Cited by 70 publications

(80 citation statements)

References 42 publications

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“…For example, L. monocytogenes can persist in a food processing facility for months and recontaminate product passing through that facility (Leong et al . ).…”

Section: Potential Sources Of L Monocytogenes Contamination In the Fmentioning

confidence: 97%

“…Leong et al . () determined that out of four food groups tested, vegetable processing facilities had the highest number of persistent strains and the highest diversity of pulsotypes which may reflect L. monocytogenes presence and distribution in the growing environment for fruit and vegetables.…”

Section: Harbourage Sites and Persistent Strains In Food Processing Fmentioning

confidence: 99%

“…Leong et al . () used PGFE to subtype isolates from a variety of food processing facilities and were able to not only identify persistent strains in vegetable processing facilities which subsequently contaminated produce but also recognize that elucidation of the specific source of contamination in a processing facility requires sampling over a longer time. Nastasijevic et al .…”

Section: Source Tracking L Monocytogenes In the Flpscmentioning

confidence: 99%

“…Survival of L. monocytogenes in these environments is key to its transmission to foodstuffs. For example, L. monocytogenes can persist in a food processing facility for months and recontaminate product passing through that facility (Leong et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Sources and survival ofListeria monocytogeneson fresh, leafy produce

Smith

Moorhouse²,

Monaghan

et al. 2018

J Appl Microbiol

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Listeria monocytogenes is an intracellular human pathogen which enters the body through contaminated food stuffs and is known to contaminate fresh leafy produce such as spinach, lettuce and rocket. Routinely, fresh leafy produce is grown and processed on a large scale before reaching the consumer through various products such as sandwiches and prepared salads. From farm to fork, the fresh leafy produce supply chain (FLPSC) is complex and contains a diverse range of environments where L. monocytogenes is sporadically detected during routine sampling of produce and processing areas. This review describes sources of the bacteria in the FLPSC and outlines the physiological and molecular mechanisms behind its survival in the different environments associated with growing and processing fresh produce. Finally, current methods of source tracking the bacteria in the context of the food supply chain are discussed with emphasis on how these methods can provide additional, valuable information on the risk that L. monocytogenes isolates pose to the consumer.

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“…For example, L. monocytogenes can persist in a food processing facility for months and recontaminate product passing through that facility (Leong et al . ).…”

Section: Potential Sources Of L Monocytogenes Contamination In the Fmentioning

confidence: 97%

Section: Harbourage Sites and Persistent Strains In Food Processing Fmentioning

confidence: 99%

Section: Source Tracking L Monocytogenes In the Flpscmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sources and survival ofListeria monocytogeneson fresh, leafy produce

Smith

Moorhouse²,

Monaghan

et al. 2018

J Appl Microbiol

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“…Nowadays, Listeria monocytogenes is still a major health issue worldwide due to its high mortality rate and ubiquity (Leong et al., ; Madden et al., ). According to the latest European Food Safety Authority report, a total of 2536 confirmed cases were reported in Europe in 2016, representing a 9.3% increase with respect to 2015.…”

Section: Introductionmentioning

confidence: 99%

Combination of Immunomagnetic Separation and Real‐Time Recombinase Polymerase Amplification (IMS‐qRPA) for Specific Detection of Listeria monocytogenes in Smoked Salmon Samples

Garrido‐Maestu

Azinheiro

Carvalho

et al. 2019

Journal of Food Science

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Nowadays, Listeria monocytogenes continues to be a major health issue. Therefore, improvements in the speed and reliability of its detection are still needed. In the present study, the combination of real‐time Recombinase Polymerase Amplification (qRPA) with immunomagnetic separation (IMS) is described. The proposed methodology was tested against a real‐time PCR method, and was successfully applied to 50 smoked salmon samples spiked at levels ranging from 2 to 9.3 × 102 cfu/25 g. L. monocytogenes was detected after a 24 hr pre‐enrichment, which represents a great improvement over other previously published RPA methods. Additionally, the evaluation of the method reported a Limit of dDetection 50 (LoD50) of 6.3 cfu/25 g, along with relative sensitivity, specificity and accuracy values higher than 90%. Finally, the index of kappa concordance was calculated to be 0.93 which is interpreted as “almost complete concordance” between the reference and alternative method. Overall, the described methodology proved to be faster, specific, and as sensitive as other methods based on RPA or real‐time PCR. Practical Application The methodology described in this study significantly reduces the detection time of L. monocytogenes, when compared with culture‐based methods, and it requires fewer steps than other molecular methods, making it a reliable and more convenient method for routine testing. Finally, the evaluation of the methodology in spiked food samples, confirms its reliability.

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Detection and Enumeration of Foodborne Pathogens and Bacterial Toxins in Food

Haddad

2022

Microbiological Risk Assessment Associated With the Food Processing and Distribution Chain

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A 3-year multi-food study of the presence and persistence of Listeria monocytogenes in 54 small food businesses in Ireland

Cited by 70 publications

References 42 publications

Sources and survival ofListeria monocytogeneson fresh, leafy produce

Sources and survival ofListeria monocytogeneson fresh, leafy produce

Combination of Immunomagnetic Separation and Real‐Time Recombinase Polymerase Amplification (IMS‐qRPA) for Specific Detection of Listeria monocytogenes in Smoked Salmon Samples

Detection and Enumeration of Foodborne Pathogens and Bacterial Toxins in Food

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