A

Buckingham, J.

doi:10.1007/978-1-4899-3316-4_1

Cited by 3 publications

(2 citation statements)

References 284 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They are hydrocarbons made up of five, or multiples of five, carbon units (Simion, 2005). In plants and fungi, they are commonly found as essential as well as secondary metabolites involved in signaling and defense (Buckingham, 1994). While members of the Actinobcteria group synthesize a plethora of natural products (Baltz, 2008), very few are known to encode diterpene (C 20 ) synthases (Dairi et al, 2001; Hamano et al, 2002; Dürr et al, 2006; Smanski et al, 2011).…”

Section: Tuberculosis and The Host Environmentmentioning

confidence: 99%

Building a better bacillus: the emergence of Mycobacterium tuberculosis

Wang¹,

Behr²

2014

Front. Microbiol.

View full text Add to dashboard Cite

The genus Mycobacterium is comprised of more than 150 species that reside in a wide variety of habitats. Most mycobacteria are environmental organisms that are either not associated with disease or are opportunistic pathogens that cause non-transmissible disease in immunocompromised individuals. In contrast, a small number of species, such as the tubercle bacillus, Mycobacterium tuberculosis, are host-adapted pathogens for which there is no known environmental reservoir. In recent years, gene disruption studies using the host-adapted pathogen have uncovered a number of “virulence factors,” yet genomic data indicate that many of these elements are present in non-pathogenic mycobacteria. This suggests that much of the genetic make-up that enables virulence in the host-adapted pathogen is already present in environmental members of the genus. In addition to these generic factors, we hypothesize that molecules elaborated exclusively by professional pathogens may be particularly implicated in the ability of M. tuberculosis to infect, persist, and cause transmissible pathology in its host species, Homo sapiens. One approach to identify these molecules is to employ comparative analysis of mycobacterial genomes, to define evolutionary events such as horizontal gene transfer (HGT) that contributed M. tuberculosis-specific genetic elements. Independent studies have now revealed the presence of HGT genes in the M. tuberculosis genome and their role in the pathogenesis of disease is the subject of ongoing investigations. Here we review these studies, focusing on the hypothesized role played by HGT loci in the emergence of M. tuberculosis from a related environmental species into a highly specialized human-adapted pathogen.

show abstract

Section: Tuberculosis and The Host Environmentmentioning

confidence: 99%

Building a better bacillus: the emergence of Mycobacterium tuberculosis

Wang¹,

Behr²

2014

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The synthesis of compounds containing the N-aryl moiety has attracted a great deal of interest recently due to the importance of such compounds in fields as diverse as natural products [1], photograph [2], and materials [3]. Palladium-catalyzed Ullmann-type coupling reactions are traditional methods to assemble these compounds and have been extensively developed in recent years [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Highly efficient copper-catalyzed N-arylation of alkylamines with aryl iodides using phosphoramidite as ligand

Zhang

Mao

Zhu

et al. 2005

Catalysis Communications

View full text Add to dashboard Cite

Progress in terpene synthesis strategies through engineering ofSaccharomyces cerevisiae

Paramasivan

Mutturi

2017

Critical Reviews in Biotechnology

112

103

View full text Add to dashboard Cite

Terpenes are natural products with a remarkable diversity in their chemical structures and they hold a significant market share commercially owing to their distinct applications. These potential molecules are usually derived from terrestrial plants, marine and microbial sources. In vitro production of terpenes using plant tissue culture and plant metabolic engineering, although receiving some success, the complexity in downstream processing because of the interference of phenolics and product commercialization due to regulations that are significant concerns. Industrial workhorses' viz., Escherichia coli and Saccharomyces cerevisiae have become microorganisms to produce non-native terpenes in order to address critical issues such as demand-supply imbalance, sustainability and commercial viability. S. cerevisiae enjoys several advantages for synthesizing non-native terpenes with the most significant being the compatibility for expressing cytochrome P450 enzymes from plant origin. Moreover, achievement of high titers such as 40 g/l of amorphadiene, a sesquiterpene, boosts commercial interest and encourages the researchers to envisage both molecular and process strategies for developing yeast cell factories to produce these compounds. This review contains a brief consideration of existing strategies to engineer S. cerevisiae toward the synthesis of terpene molecules. Some of the common targets for synthesis of terpenes in S. cerevisiae are as follows: overexpression of tHMG1, ERG20, upc2-1 in case of all classes of terpenes; repression of ERG9 by replacement of the native promoter with a repressive methionine promoter in case of mono-, di- and sesquiterpenes; overexpression of BTS1 in case of di- and tetraterpenes. Site-directed mutagenesis such as Upc2p (G888A) in case of all classes of terpenes, ERG20p (K197G) in case of monoterpenes, HMG2p (K6R) in case of mono-, di- and sesquiterpenes could be some generic targets. Efforts are made to consolidate various studies (including patents) on this subject to understand the similarities, to identify novel strategies and to contemplate potential possibilities to build a robust yeast cell factory for terpene or terpenoid production. Emphasis is not restricted to metabolic engineering strategies pertaining to sterol and mevalonate pathway, but also other holistic approaches for elsewhere exploitation in the S. cerevisiae genome are discussed. This review also focuses on process considerations and challenges during the mass production of these potential compounds from the engineered strain for commercial exploitation.

show abstract

A

Cited by 3 publications

References 284 publications

Building a better bacillus: the emergence of Mycobacterium tuberculosis

Building a better bacillus: the emergence of Mycobacterium tuberculosis

Highly efficient copper-catalyzed N-arylation of alkylamines with aryl iodides using phosphoramidite as ligand

Progress in terpene synthesis strategies through engineering ofSaccharomyces cerevisiae

Contact Info

Product

Resources

About