A 3D cell printed muscle construct with tissue-derived bioink for the treatment of volumetric muscle loss

Choi, Yeong Jin; Jun, Young Joon; Kim, Dong Yeon; Yi, Hee; Chae, Su Hun; Kang, Jun Su; Lee, Ju Yong; Gao, Ge; Kong, Jeong Sik; Jang, Jae-Won; Chung, Wan Kyun; Rhie, Jong Won; Cho, Dong‐Woo

doi:10.1016/j.biomaterials.2019.03.036

Cited by 243 publications

(224 citation statements)

References 32 publications

Supporting

Mentioning

222

Contrasting

Order By: Relevance

“…In addition, a neonatal-scale human heart was printed, and patient-specific anatomical structure was determined by micro-CT imaging. In another example of printing using a gelatin-based support bath, the 3D cell-printed skeletal muscle construct was successfully printed while maintaining its organized volumetric structure that was mainly composed of muscle-derived tissue-specific bioink [ 100 ]. In vitro and in vivo results for the model showed high viability, aligned muscle fiber structure, and restoration of volumetric muscle loss from an injured tibialis anterior.…”

Section: Printing Methodsmentioning

confidence: 99%

“…When the 3D-printed muscle structures were implanted in a rat model of volumetric muscle loss, vascularization, innervation, and muscle contraction recovery were improved in the coaxial nozzle printing group compared to other groups. This study showed that an implant structure with spatially controlled, tissue-specific bioink and cells provides organized microenvironmental cues to differentiate each cell type, leading to more effective muscle regeneration ( Figure 3 A) [ 100 ].…”

Section: Therapeutic Applicationsmentioning

confidence: 99%

“…For this objective, current research has used sacrificial bioink to fabricate the hollow structure, and then endothelial cells are seeded on the wall to form the endothelial layer [ 92 , 96 ]. The pre-vascularized muscle construct was fabricated using a coaxial nozzle to print muscle and endothelium as the core and shell structure, respectively [ 100 ].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

“…Overall views of the constructs (left). Cross-sectional views of the constructs (right); (iii) IF images of the different muscle constructs; (iv) Twitch and tetanus forces of the printing, printing (mixed), and coaxial printing groups (n = 5); * p < 0.05, ** p < 0.01, and *** p < 0.001 (reproduced with permission from ref [ 100 ]; copyright 2019 Elsevier). ( B ) 3D printed complex tissue construct for cardiac repair.…”

Section: Figurementioning

confidence: 99%

See 3 more Smart Citations

3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications

Kim

Kong

Han

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

The development of artificial tissue/organs with the functional maturity of their native equivalents is one of the long-awaited panaceas for the medical and pharmaceutical industries. Advanced 3D cell-printing technology and various functional bioinks are promising technologies in the field of tissue engineering that have enabled the fabrication of complex 3D living tissue/organs. Various requirements for these tissues, including a complex and large-volume structure, tissue-specific microenvironments, and functional vasculatures, have been addressed to develop engineered tissue/organs with native relevance. Functional tissue/organ constructs have been developed that satisfy such criteria and may facilitate both in vivo replenishment of damaged tissue and the development of reliable in vitro testing platforms for drug development. This review describes key developments in technologies and materials for engineering 3D cell-printed constructs for therapeutic and drug testing applications.

show abstract

Section: Printing Methodsmentioning

confidence: 99%

Section: Therapeutic Applicationsmentioning

confidence: 99%

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

See 2 more Smart Citations

3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications

Kim

Kong

Han

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, cells were encapsulated in a microenvironment similar to that of the native ECM, offering an additional advantage for myogenesis [ 24 ]. The same group demonstrated that prevascularized 3D printed muscle constructs co-axially fabricated with muscle and vascular dECM bioinks successfully mimicked the hierarchical architecture of vascularized muscles with improved de novo fiber formation, vascularization, innervations and 85% functional recovery in volumetric muscle loss injuries [ 25 ]. SM dECM has also been chemically modified to impart photo-crosslinkable properties and thus to enhance mechanical stability.…”

Section: Moving Towards Biomimetic Engineered Muscular Constructsmentioning

confidence: 99%

Information-Driven Design as a Potential Approach for 3D Printing of Skeletal Muscle Biomimetic Scaffolds

Baiguera

Gaudio²,

Carotenuto

et al. 2020

Nanomaterials

View full text Add to dashboard Cite

Severe muscle injuries are a real clinical issue that still needs to be successfully addressed. Tissue engineering can represent a potential approach for this aim, but effective healing solutions have not been developed yet. In this regard, novel experimental protocols tailored to a biomimetic approach can thus be defined by properly systematizing the findings acquired so far in the biomaterials and scaffold manufacturing fields. In order to plan a more comprehensive strategy, the extracellular matrix (ECM), with its properties stimulating neomyogenesis and vascularization, should be considered as a valuable biomaterial to be used to fabricate the tissue-specific three-dimensional structure of interest. The skeletal muscle decellularized ECM can be processed and printed, e.g., by means of stereolithography, to prepare bioactive and biomimetic 3D scaffolds, including both biochemical and topographical features specifically oriented to skeletal muscle regenerative applications. This paper aims to focus on the skeletal muscle tissue engineering sector, suggesting a possible approach to develop instructive scaffolds for a guided healing process.

show abstract

Bioprinting Tissue Analogues with Decellularized Extracellular Matrix Bioink for Regeneration and Tissue Models of Cartilage and Intervertebral Discs

Vernengo

Grad

Eglin

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

The recent convergence of decellularized extracellular matrix (dECM) methodologies and 3D bioprinting (3DBP) has led to multiple advancements in tissue engineering scaffold design by enabling researchers to recreate a tissue-specific 3D environment in a custom geometry. The application of 3DBP of dECM for articular cartilage and intervertebral disc (IVD) repair, however, is still burgeoning. While cartilage and IVD tissue each possess unique architectures, they are composed of similar macromolecules and therefore pose similar challenges for the successful application of their matrix bioinks. Herein, the state-of-the-art in cartilage and IVD dECM bioink preparation, material properties, and applications are highlighted. The current major obstacles regarding optimal decellularization and printing methods are discussed, which need to be overcome to enable recapitulation of the hierarchical organization, zone-specific matrix composition, and anisotropic biomechanical behavior of the native tissues. Finally, a vision is presented for how this field may continue to evolve in the future to empower the fabrication of scaffolds that serve as effective templates for guiding cellular assembly and organization toward the formation of functional cartilage and IVD tissues.

show abstract

A 3D cell printed muscle construct with tissue-derived bioink for the treatment of volumetric muscle loss

Cited by 243 publications

References 32 publications

3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications

3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications

Information-Driven Design as a Potential Approach for 3D Printing of Skeletal Muscle Biomimetic Scaffolds

Bioprinting Tissue Analogues with Decellularized Extracellular Matrix Bioink for Regeneration and Tissue Models of Cartilage and Intervertebral Discs

Contact Info

Product

Resources

About