A 4‐Methylumbelliferone‐based Fluorescent Probe for Sodium New Houttuyfonate

Abstract

A new fluorogenic probe for sodium new houttuyfonate (SNH) was proposed. 4-Methylumbelliferyl-2,4-dinitrobenzenesulfonate (4-MUDNBS) was a nonfluorescent compound and was synthesized via the one-step reaction of 4-methylumbelliferone (4-MU) with 2,4-dinitrobenzenesulfonyl chloride. In basic media, SNH was decomposed to produce sodium sulfite, which then reacted with 4-MUDNBS to yield highly fluorescent 4-MU, hence leading to the fluorescence increase of the reaction solution. A linear correlation existed between the emission intensity and the concentration of SNH within the range from 0.5 to 15 µg•mL -1 with a detection limit of 0.15 µg• mL -1 (3σ). The effect of substituents on the benzenesulfonyl moiety of the probe is discussed, and the presence of electronegative groups is favorable for the proposed cleavage reaction.

Guest‐Release Control in Enzyme‐Sensitive, Amphiphilic‐Dendrimer‐Based Nanoparticles through Photochemical Crosslinking

“…The hydroxycoumarin derivative, 4-methylumbelliferone (MUF), is highly fluorescent; however, the ester derivative of this molecule is not fluorescent. [10] Therefore we sought to utilize the esterase-induced cleavage of the ester functionality in molecule A (Figure 2), as the fluorescence reporting event. However, we found the hydrolytic stability of the phenolic ester in A to be poor even in the absence of the enzyme.…”