A 53 kDa protein of Vibrio cholerae classical strain 0395 involved in intestinal colonization

Singh, Shubhra; Srivastava, Ranjana; Sinha, V. B.; Srivastava, Bhavana

doi:10.1006/mpat.1994.1053

Cited by 16 publications

(28 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…of J ! cholerae [ 10,13,191. With the same strategy, a panel of mutants was isolated in which the integration Of transPoson Occurred in-frame in genes encoding…”

Section: Discussionmentioning

confidence: 99%

Screening of TnPhoA Mutants of Vibrio Cholerae O139 for Identification of Antigens Involved in Colonisation

Bondre

Srivastava²,

Sinha³

et al. 1997

Journal of Medical Microbiology

View full text Add to dashboard Cite

A new serogroup of Vibrio cholerae non-01, designated as 0139, has emerged causing cholera-like disease among adults. Laboratory and field studies clearly show that there is no cross-protection between 01 and 0139 pathogenic strains. Since colonisation of the intestine is a most important step in the pathogenesis of cholera caused by 01 strains and colonising antigens are known to be protective, investigation of the colonising antigens of 0139 strain was initiated. By TnphoA mutagenesis, mutants were generated with insertions in the genome encoding membrane spanning or secretory proteins. Screening of the mutants for adherence to rabbit intestinal surface and colonisation in 5-day-old mice resulted in the identification of mutant clones, which were less adhesive than was the wild-type parent strain and which could not efficiently colonise the gut. Such non-colonising strains were attenuated in virulence. Analysis of the proteins by SDS-PAGE revealed that the non-colonising membrane protein.

show abstract

“…of J ! cholerae [ 10,13,191. With the same strategy, a panel of mutants was isolated in which the integration Of transPoson Occurred in-frame in genes encoding…”

Section: Discussionmentioning

confidence: 99%

Screening of TnPhoA Mutants of Vibrio Cholerae O139 for Identification of Antigens Involved in Colonisation

Bondre

Srivastava²,

Sinha³

et al. 1997

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…A spontaneous streptomycin-resistant derivative, CD81 of V. cholerae 0395, was used as parent strain (Singh et al, 1994). CD83 was isolated by transposon mutagenesis of CD81 and characterized as salt sensitive.…”

Section: Methodsmentioning

confidence: 99%

“…All bacterial strains and plasmids used in this investigation are listed in Table 1. Bacteria were stored at À70 8C in LB medium containing 50 % glycerol and grown in Luria-Bertani (LB) or brain-heart infusion (BHI) or Terrific broth (TB) (Singh et al, 1994). Plates were made with 1·5 % bacto agar.…”

Section: Methodsmentioning

confidence: 99%

“…Bacteria-free culture filtrate was obtained by membrane filtration (Millipore; 0·22 ìm pore size). The presence of toxin was assayed in (Craig, 1965;Singh et al, 1994). (2) Toxin was estimated by ELISA essentially as described previously (Harlow & Lane, 1988;Singh et al, 1994).…”

Section: Methodsmentioning

confidence: 99%

“…The presence of toxin was assayed in (Craig, 1965;Singh et al, 1994). (2) Toxin was estimated by ELISA essentially as described previously (Harlow & Lane, 1988;Singh et al, 1994). Culture filtrate was serially diluted and coated in immunoplate wells (NUNC) and titrated using rabbit anti-cholera toxin IgG and horseradish-peroxidase-conjugated goat anti-rabbit IgG (Sigma) and O-phenylenediamine dihydrochloride as substrate.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Mutation in tcpR gene (Vc0832) of Vibrio cholerae O1 causes loss of tolerance to high osmolarity and affects colonization and virulence in infant mice

Mishra

Srivastava

Pruzzo

et al. 2003

Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

Vibrio cholerae, the agent of cholera, multiplies and colonizes human intestinal tract where it survives high osmolarity due to bile and other sodium salts. In this work, by TnphoA mutagenesis, a mutant of V. cholerae O1 which could not grow and form colonies on LB agar containing 400 mM NaCl has been characterized. The mutant, designated CD83, adhered normally to freshly isolated rabbit intestinal discs, colonized poorly the gut of infant mice and was avirulent in the same model, whereas the parent strain CD81 would colonize the gut and cause death of mice. Attenuation of virulence of CD83 was not attributable to its inability to produce cholera toxin, as no difference was found in the cholera toxin produced in vitro by the mutant and parent strains. Molecular cloning and sequencing of the mutated gene revealed that insertion of transposon occurred in tcpR gene (Vc0832) of V. cholerae. Complementation of the mutant with wild-type tcpR gene resulted in restoration of the ability to survive at high salt concentration (400 mM NaCl), and to colonize the gut and restore virulence. The results suggest that tcpR plays a role in survival of V. cholerae in the small intestine of host as the osmolarity in the intestinal lumen is thought to be equivalent to 300 mM NaCl or higher.

show abstract

Cholera and Drinking Water

Srivastava¹

2013

Water and Health

View full text Add to dashboard Cite

A 53 kDa protein of Vibrio cholerae classical strain 0395 involved in intestinal colonization

Cited by 16 publications

References 0 publications

Screening of TnPhoA Mutants of Vibrio Cholerae O139 for Identification of Antigens Involved in Colonisation

Screening of TnPhoA Mutants of Vibrio Cholerae O139 for Identification of Antigens Involved in Colonisation

Mutation in tcpR gene (Vc0832) of Vibrio cholerae O1 causes loss of tolerance to high osmolarity and affects colonization and virulence in infant mice

Cholera and Drinking Water

Contact Info

Product

Resources

About