A 96-well microplate incorporating a replica molded microfluidic network integrated with photonic crystal biosensors for high throughput kinetic biomolecular interaction analysis

Choi, Charles J.; Cunningham, Brian T.

doi:10.1039/b618584c

Cited by 90 publications

(66 citation statements)

References 20 publications

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“…We compare our sensing platform to another robust, multiplexable photonic crystal based sensing platform developed by Choi et al 18 In their work they report observing a 1 nm red-shift while detecting the association of 500 mg/ml of rabbit IgG (Immunoglobin G). In our experiments, we have used rabbit IgG anti-streptavidin which possesses a similar molecular weight.…”

Section: Discussionmentioning

confidence: 99%

A multiplexed optofluidic biomolecular sensor for low mass detection

2009

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Optical techniques have proven to be well suited for in situ biomolecular sensing because they enable high fidelity measurements in aqueous environments, are minimally affected by background solution pH or ionic strength, and facilitate label-free detection. Recently, there has been significant interest in developing new classes of optically resonant biosensors possessing very high quality-factors. This high quality-factor enables them to resolve the presence of very small amounts of bound mass and leads to very low limits of detection. A drawback of these devices is that the majority of the resonant electromagnetic energy is confined within the solid light-guiding structure thus limiting the degree to which it overlaps with the bound matter. This in turn lowers the ultimate device sensitivity, or the change in output signal in response to changes in bound mass. Here we present a novel optofluidic biosensor platform that incorporates a unique one-dimensional photonic crystal resonator array which enables significantly stronger light-matter interaction. We show here how this, coupled with the ability of planar photonic crystals to spatially localize the optical field to mode volumes on the order of a wavelength cubed, enables a limit of detection on the order of 63 ag total bound mass (estimated using a polyelectrolyte growth model) and a device sensitivity an order of magnitude better than similar devices. The multiplexing capabilities of our sensor are demonstrated by the individual and concurrent detection of interleukins 4, 6 and 8 using a sandwich assay.

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Section: Discussionmentioning

confidence: 99%

A multiplexed optofluidic biomolecular sensor for low mass detection

2009

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“…One such example of a label-free biosensor suitable for 96-, 384-, and 1536-well plate screening is a photonic crystal or resonant waveguide grating (RWG) biosensor (16,17). The RWG is composed of a periodic arrangement of dielectric material with appropriate periodicity and symmetry to induce selective coupling of energy at particular wavelengths, while excluding others.…”

Section: Resonant Waveguide Grating Biosensorsmentioning

confidence: 99%

Signal transduction profiling using label-free biosensors

Cooper¹

2009

Journal of Receptors and Signal Transduction

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Knowledge of the way in which ligands modulate cellular responses via membrane-associated receptors is of central importance to drug discovery and elucidation of signal transduction pathways. Biophysical label-free methods can be used to characterize ligand and drug candidate interactions with neurotransmitters, cytokine receptors, tyrosine kinase receptors, ligand- and voltage-gated ion channels, G protein-coupled receptors (GPCRs), and antibody receptors. Ligand or drug candidate screening typically involves selecting ligands or subsets of a compound library for analysis, transfecting a cell line overexpressing the target receptor, then monitoring one or two downstream reporters of receptor activation such as Ca(2+), cAMP, inositol phosphate, etc. Inevitably, this process leads to a data set predicated by these selections. In contrast, label-free screening techniques allow a holistic, pathway-independent screening strategy to provide a functional or phenotypic readout of receptor activation. Detection techniques that measure changes in cell conductance, viscoelastic properties, refractive index, and other optical parameters that are modulated as a consequence of receptor activation are reviewed.

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“…3, typical optical detection methods comprise the direct detection by monitoring the light properties including fluorescence, 74,[81][82][83] absorbance, [84][85][86] and luminescence-based [87][88][89] methods, and the light property modulation detections such as surface plasmon 78 and optical waveguides. 79,132 Photonic crystals, optical cavity structures, and several optical techniques have also been reported to be integrated with the microfluidic system for sensing purposes, using one-and two-dimensional surface PCs (guided mode resonance filters), [133][134][135][136] , or three-dimensional photonic crystals, 137,138 optical cavities, 139,140 whispering gallery mode resonators, [141][142][143][144][145][146][147] and optical tweezers for cell related monitoring or fluidic rheological measurements [148][149][150] (Fig. 4).…”

Section: Detection Methodsmentioning

confidence: 99%