A bacterial bioassay for measuring the copper‐chelation capacity of seawater 1

Gillespie, Paul; Vaccaro, Ralph F.

doi:10.4319/lo.1978.23.3.0543

Cited by 74 publications

(14 citation statements)

References 2 publications

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“…The solid phase was removed by filtering the coffee suspension through Whatman 114 filter paper before adding 10.1 g of KNO 3 to make a final ionic strength I = 0.1 mol L -1 . After the KNO 3 was dissolved the solution was divided into 50 mL portions in beakers.…”

Section: Preparation Of Copper and Coffee Solutionsmentioning

confidence: 99%

Reducing copper toxicity by drinking coffee

Utomo¹

2008

WIT Transactions on Ecology and the Environment

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Section: Preparation Of Copper and Coffee Solutionsmentioning

confidence: 99%

Reducing copper toxicity by drinking coffee

Utomo¹

2008

WIT Transactions on Ecology and the Environment

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“…Various biological responses have been used to analyze water quality, among them survival (Anderson and Abdelghani, 1980), growth (Bringmann and Kiihn, 1979), ATP (yennicut, 1980), oxygen uptake (Plotz, 1974), and nutrient absorption (Albright and Wilson, 1974;Gillespie and Vaccaro, 1978;Bossard and Gachter, 1979;Sayler et al, 1979).…”

Section: Bioassaysmentioning

confidence: 99%

Mercury Influences Uptake of Amino Acids by Marine Bacteria

Siebers¹,

Hoppenheit²,

Harms³

1981

Mar. Ecol. Prog. Ser.

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Natural multispecies bacterial communities, in a resting state and at low initial densities. were used to study effects of dissolved mercury on nutrient absorption. Nutrients were offered as 10 pM amino-acid mixture, in the relative proportions encountered in natural sea-water samples. Uptake, commencing after an initial stationary phase, resulted from induction of transport mechanisms in a relatively constant number of bacteria, rather than from increased densities due to multiplication following nutrient availability. The periods elapsed to reduce ambient amino-acid concentrations to half of the initially present levels were greatly influenced by the presence of low levels of Hg, revealing significant uptake inhibition at a sensitivity threshold of 0.625 pg I-' Hg. Low Hg levels affected induction and uptake in dormant bacteria, but had no effect on uptake in a high density community in the logarithmic phase. Higher levels of up to 488 yg I-' Hg greatly decreased rates of uptake and prolonged the duration of lag phases, obviously due to changes in species composition. Use of natural multispecies communities allowed the simultaneous quantitative detection of a sequence of different Hg levels, varying from lowest (0.625) to highest (488 kg I-' Hg) ambient concentrations. Initially present levels of total (2.5 bg I-') and dissolved Hg decreased rapidly, while mercury associated with the particulate material increased to constant values between 12 and 16 h after the start of experiments. Our findings indicate that toxicity is less due to concentration of Hg alone, but rather depends on the relation between concentration and biomass. Because of unavoidable changes in sea-water properties resulting from handling between sampling and start of the tests (transport, storage and freeing of collected samples from originally present bacteria), the procedure is probably not appropriate for detecting subtle differences in water quality present in the sea at dumping sites and in adjacent polluted areas.

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“…The importance of the latter difficulty is emphasized by studies suggesting that copper binding organic ligands are present in sufficient quantities to effect the speciation of copper in seawater Florence and Batley, 1976;Williams and Baldwin, 1976;Sugai and Healy, 1978). More specifically, the results of Sugai and Healy (1978) and Gillespie and Vaccaro (1978) indicate that dissolved organic matter in Saanich Inlet waters exerts a significant influence on the speciation of copper.…”

Section: Introductionmentioning

confidence: 99%

Toxicity of a Mixture of Ten Metals to Phytoplankton

Thomas¹,

Hollibaugh²,

Seibert³

et al. 1980

Mar. Ecol. Prog. Ser.

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We studied effects of a mixture of ten heavy metals (Cu, Zn, Ni, Cr, Pb, Cd, Hg, As, Sb and Se) on phytoplankton growth in a natural assemblage of diatoms and dinoflagellates from Saanich Inlet, B. C. (Canada) and in laboratory cultures of the diatom Thalassiosira aestivalis. Growth was not inhibited at metal concentrations expected to occur in a moderately polluted estuary. However, at 5-or 10-fold higher metal concentrations, phytoplankton growth was inhibited both in natural populations and in laboratory cultures. Inhibition of natural assemblages could be attributed to Cu and Hg; deletion of these elements resulted in growth equivalent to that of control cultures containing no metals. Similarly, inhibition of T. aestivalis cultures was due to Cu in the metal mixture. With natural assemblages certain diatoms such as Chaetocerossp., Rhizosolenia sp. or Nitzschia delicatissima were no longer dominant in cultures containing a metal mixture 5 times that in controls.

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A bacterial bioassay for measuring the copper‐chelation capacity of seawater 1

Cited by 74 publications

References 2 publications

Reducing copper toxicity by drinking coffee

Reducing copper toxicity by drinking coffee

Mercury Influences Uptake of Amino Acids by Marine Bacteria

Toxicity of a Mixture of Ten Metals to Phytoplankton

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