A bacterial consortium isolated from an Icelandic fumarole displays exceptionally high levels of sulfate reduction and metals resistance

Alexandrino, Maria; Macías, Francisco; Costa, Rodrigo; Gomes, Newton C. M.; Canário, Adelino V.M.; Costa, Maria Clara

doi:10.1016/j.jhazmat.2011.01.035

Cited by 27 publications

(17 citation statements)

References 42 publications

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“…The metal pollutants may inactivate enzymes, denature the proteins and enter in competition with essential cations (Utgikar et al 2002 ). But in a study reported earlier by Alexandrino et al ( 2011 ), SRB showed maximum tolerance and activity even at extra ordinary high concentration of copper (more than 500 ppm). However, those bacteria were procured from a highly metal polluted environment where copper concentration was more than 1000 mg/kg (1000 ppm) and therefore, they must have tolerance against much higher concentrations of copper.…”

Section: Discussionmentioning

confidence: 74%

“…The major advantages of the biological methods are their better performance, low operational cost, minimum generation of secondary pollutants and environmentally compatible natures (Malik 2004 ; Okoh and Trejo-Hernandez 2006 ). Implication of dissimilatory sulphate-reducing bacteria (DSRB) in detoxifying metal contaminated environments has gained importance among all the biological methods practiced for the removal of metals in the last decade (Neculita et al 2007 ; Martins et al 2009 ; Alexandrino et al 2011 ; Barbosa et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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Metals-induced functional stress in sulphate-reducing thermophiles

Hussain

Qazi

2016

3 Biotech

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All toxic metals have been known to inhibit different activities of sulphate-reducing bacteria (SRB) at different concentrations. The present study delineates functional responses of two thermophilic SRB species (Desulfotomaculum reducens-HA1 and Desulfotomaculum hydrothermale-HA2) to toxic metals. Bacterial activity was assessed in terms of sulphate reduction and metal precipitation employing four concentrations (1, 5, 10 and 15 ppm) of three dissolved toxic metals (Cu, Cr and Ni) independently. Both sulphidogenic bacterial species showed results in a very narrow range of fluctuations. In general, bioprecipitation and sulphate reduction were pronounced at lower concentrations (1 and 5 ppm) and got inhibited at higher concentrations (10 and 15 ppm). The order of precipitation and sulphate reduction for the subject metals was Ni > Cr > Cu. The findings of this study will be helpful in developing economical and environmental friendly bioremediation process(es) tending to operate at extreme conditions around the concentrations in indicated suitable metals-loaded effluents.

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Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Metals-induced functional stress in sulphate-reducing thermophiles

Hussain

Qazi

2016

3 Biotech

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“…To ensure the success of the biological AMD treatment with such sustainable substrates, therefore, cellulose‐degrading bacteria (e.g., Cellulomonas , Bacteroides spp., and Clostridium sp.) should be included in an AMD treatment system and actively interact with SRBs (Pruden et al, 2007; Alexandrino et al, 2011). This interaction between SRBs and cellulose‐degraders can enable the operation of the bioreactor for extended periods because cellulosic component as organic substrate persistently comes from nature (Pruden et al, 2007).…”

mentioning

confidence: 99%

Biosulfides Precipitation in Weathered Tailings Amended with Food Waste-based Compost and Zeolite

2012

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Tailings are mine wastes in the form of slurries stacked in mine sites abandoned after the exhaustion of ores. There are approximately 5000 abandoned mine sites in Korea, and tailings have become a serious environmental problem. Long‐term environmental exposure of tailings can cause release of acidic and high concentrations of sulfate‐ and metal‐contaminated water (acid mine drainage, AMD). Organic and/or inorganic amendments have been studied for AMD prevention and passive in situ treatment of pore water. This study tests locally available food waste‐based compost as a viable amendment, in addition to the need for sustainable ways to dispose of compost, in response to a new environmental law. To examine the feasibility, three bioreactors were constructed, filled with mixtures of tailings, food waste‐based compost, and zeolite. During the 4‐wk experimental period, feeding water ormedium were poured in one reactor. The leachates were investigated in terms of chemistry and microbiology. Compared with the unamended reactor, the leachate from two mixture‐filled reactors showed increased pH, formation of sulfate reduction conditions, and highly efficient metal removal. Black‐colored precipitates observed at the end of the experiment suggested the formation of metal biosulfides, following the activity of sulfate reduction mediated by sulfate‐reducing bacteria (SRB). Mineralogical analysis of these precipitates confirmed the presence of biosulfides, mainly of Fe and Pb. Moreover, microbial and molecular biological analyses revealed that several species of heterotrophic bacteria (SRB and iron‐reducing bacteria) were present in the solids recovered from the bioreactors. Microbial consortium, such as SRB species (Desulfotomaculum putei), and cellulosic‐degrader (Ruminococcus sp.) were identified. This study provides promising results on the application potential of food waste‐based compost for prevention of AMD generation and passive in situ treatment of pore water in weathered tailings in Korea and elsewhere.

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“…La DGGE es el método de elección cuando la información que se necesita no es tan profunda desde el punto de vista filogenético como la proporcionada por una biblioteca de clones, pero precisa como para determinar los miembros dominantes de una comunidad microbiana. Un simple análisis de patrones de bandas puede ser suficiente para determinados fines como observar los cambios espaciales y temporales de un consorcio (Dar et al, 2008), detectar la presencia de determinado organismo (Teske et al, 1996) o observar el efecto de una variable sobre la composición de un consorcio (Alexandrino et al, 2011). Sin embargo, en general esta técnica se combina con otros métodos.…”

Section: Biorreactores Sulfidogénicosunclassified

“…Sin embargo, sólo algunos autores aplicaron estos métodos al estudio del efecto de los metales pesados y el pH sobre las comunidades bacterianas. Alexandrino et al (2011) realizaron un análisis de DGGE para determinar el efecto de la presencia de 0,75 g/L de hierro, 0,20 g/L de zinc y 0,080 g/L de cobre sobre la composición de una comunidad bacteriana obtenida de una fumarola con alto contenido metálico. Observaron que, a pesar de que no se registraron efectos inhibitorios en la reducción de sulfato ni una disminución de la carga bacteriana por la presencia de los metales, la diversidad bacteriana decreció drásticamente.…”

Section: Aislamiento De Cepas De Brs Y Caracterizaciónunclassified

Bioprecipitación de metales pesados por bacterias reductoras de sulfato aisladas de muestras ambientales

Kikot¹

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El azufre, uno de los elementos más abundantes en la Tierra, puede ser transformado química y biológicamente. El sulfato es tomado como nutriente, reducido a sulfuro e incorporado luego en aminoácidos azufrados y enzimas, en un proceso que se conoce como reducción asimilatoria de sulfato. La reducción asimilatoria de sulfato no libera sulfuro al ambiente, a excepción de la putrefacción de los organismos muertos (Rabus et al., 2006). Las reacciones de oxidación y la reducción para la generación de energía metabólica son también importantes, como la reducción no asimilatoria de sulfato realizado por las bacterias reductoras de sulfato (BRS), microorganismos anaerobios que utilizan este compuesto como aceptor final de electrones, lo que resulta en la producción de sulfuro. Dado que miembros de los dominios de las Bacterias y las Arqueas puede llevar a cabo este proceso, algunos investigadores usan el término procariotas reductoras de sulfato o microorganismos reductores de sulfato. Sin embargo, se utiliza usualmente el término BRS para referirse a ambos dominios (Muyzer y Stams, 2008). El objetivo de esta tesis es desarrollar sistemas de remediación biológica de metales pesados utilizando BRS obtenidas de muestras ambientales.

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A bacterial consortium isolated from an Icelandic fumarole displays exceptionally high levels of sulfate reduction and metals resistance

Cited by 27 publications

References 42 publications

Metals-induced functional stress in sulphate-reducing thermophiles

Metals-induced functional stress in sulphate-reducing thermophiles

Biosulfides Precipitation in Weathered Tailings Amended with Food Waste-based Compost and Zeolite

Bioprecipitación de metales pesados por bacterias reductoras de sulfato aisladas de muestras ambientales

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