A bactericidal product obtained from a mutant of escherichia coli

Zwaig, N.; Diéguez, Eugenia

doi:10.1016/0006-291x(70)90025-2

Cited by 15 publications

(7 citation statements)

References 5 publications

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“…The, observed inhibition of RNA synthesis (Fig. 2) is not in agreement with previous results (16), which show a stimulation rather than an inhibition by BPG. This difference can be attributed to the lack of a carbon source in the experiments previously performed.…”

Section: Resultscontrasting

confidence: 62%

“…The compound which produces this bactericidal effect is released into the medium and can be separated from the cells (16). Some characteristic of this bactericidal product (BPG) have been reported in a previous paper (16): it is a small molecule produced by Hfr and F+ but not by F-strains; it has lethal activity on gram-positive and gram-negative bacteria; it inhibits in vivo protein synthesis.…”

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confidence: 91%

“…When cells of this strain are grown on a mineral medium plus a carbon source, e.g., succinate, the addition of glycerol stops further growth, and a few hours afterwards no viable cells can be detected by using rich medium plates (17). The compound which produces this bactericidal effect is released into the medium and can be separated from the cells (16). Some characteristic of this bactericidal product (BPG) have been reported in a previous paper (16): it is a small molecule produced by Hfr and F+ but not by F-strains; it has lethal activity on gram-positive and gram-negative bacteria; it inhibits in vivo protein synthesis.…”

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confidence: 99%

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Properties and Mode of Action of a Bactericidal Compound (= Methylglyoxal) Produced by a Mutant of Escherichia coli

et al. 1971

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A lethal product (BPG) produced by a glycerol kinase mutant of Escherichia coli was purified, and its mode of action on E. coli was studied. At concentrations where BPG strongly inhibits in vivo deoxyribonucleic acid, ribonucleic acid, and protein synthesis, it produces small effects on other functions: slight inhibition of respiration and small changes in intracellular pools of substrates, nucleic acids degradation, and adenosine triphosphate levels. BPG also inhibits in vitro protein synthesis and produces inactivation of bacteriophage T4. The bactericidal product has been identified in another laboratory as methylglyoxal (MG). By comparing BPG and MG, we confirmed this observation and concluded that the activity found in our BPG preparation is due to its MG content. We also observed that MG is able to react with guanosine triphosphate. According to these results, it is interpreted that MG could act directly on macromolecular synthesis by reacting with the guanine residues of nucleic acids and its precursors. All the other chemicals employed were of the highest purity commercially available.

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Section: Resultscontrasting

confidence: 62%

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confidence: 91%

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confidence: 99%

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Properties and Mode of Action of a Bactericidal Compound (= Methylglyoxal) Produced by a Mutant of Escherichia coli

et al. 1971

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“…and collaborators (14, 32) and in the in vivo experiments of Zwaig and Dieguez (43). However, there is also evidence for methylglyoxal inhibition of succinate oxidase and a number of other enzymes (13,29).…”

Section: Discussionmentioning

confidence: 99%

“…2 Present address: Ben May Laboratory for Cancer Research and the Department of Biochemistry, The University of Chicago, Chicago, III. 60637. this strain, suspended in an unbuffered solution of glycerol, excreted a dialyzable product which is toxic to a variety of bacterial cells, including both gram-positive and gram-negative species (43). These workers found the bactericidal product to be stable to heating at 100 C for 10 min and to have a molecular weight of less than 500 by gel-exclusion chromatography (43).…”

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Lethal Synthesis of Methylglyoxal by Escherichia coli During Unregulated Glycerol Metabolism

1971

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In Escherichia coli K-12, the conversion of glycerol to triose phosphate is regulated by two types of control mechanism: the rate of synthesis of glycerol kinase and the feedback inhibition of its activity by fructose-1,6-diphosphate. A strain which has lost both control mechanisms by successive mutations, resulting in the constitutive synthesis of a glycerol kinase no longer sensitive to feedback inhibition, can produce a bactericidal factor from glycerol. This toxic factor has been identified by chemical and enzymological tests as methylglyoxal. Methylglyoxal can be derived from dihydroxyacetone phosphate through the action of an enzyme which is present at high constitutive levels in the extracts of the mutant as well as that of the wild-type strain. Nine spontaneous mutants resistant to I mm exogenous methylglyoxal have been isolated. In all cases the resistance is associated with increased levels of a glutathione-dependent enzymatic activity for the removal of methylglyoxal. Methylglyoxal-resistant mutants derived from the glycerol-sensitive parental strain also became immune to glycerol.

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Nonenzymic formation of toxic levels of methylglyoxal from glycerol and dihydroxyacetone in Ringer's phosphate suspensions of avian spermatozoa

Riddle

Lorenz

1973

Biochemical and Biophysical Research Communications

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A bactericidal product obtained from a mutant of escherichia coli

Cited by 15 publications

References 5 publications

Properties and Mode of Action of a Bactericidal Compound (= Methylglyoxal) Produced by a Mutant of Escherichia coli

Properties and Mode of Action of a Bactericidal Compound (= Methylglyoxal) Produced by a Mutant of Escherichia coli

Lethal Synthesis of Methylglyoxal by Escherichia coli During Unregulated Glycerol Metabolism

Nonenzymic formation of toxic levels of methylglyoxal from glycerol and dihydroxyacetone in Ringer's phosphate suspensions of avian spermatozoa

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