A barley flour inhibitor of insect α‐amylase is a major allergen associated with baker's asthma disease

Barber, Domingo; Sánchez‐Monge, Rosa; Gómez, Luis; Carpizo, J. A.; Armentia, Alicia; López-Otı́n, Carlos; Juan, F; Salcedo, Gabriel

doi:10.1016/0014-5793(89)80444-2

Cited by 74 publications

(58 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of these inhibitors isolated from wheat, barley and rice are recognized by specific IgE when tested with sera from allergic patients (Barber et al, 1989;García-Casado et al, 1995;Gómez et al, 1990;Izumi et al, 1992;Sanchez-Monge et al, 1992;1996a,b). However, their IgE-binding capacities in vi tro are very different .…”

Section: Allergenic Propertíesmentioning

confidence: 99%

A Multigene Family of Trypsin/α-Amylase Inhibitors from Cereals

Carbonero¹,

Garcı́a-Olmedo²

1999

Seed Proteins

View full text Add to dashboard Cite

Section: Allergenic Propertíesmentioning

confidence: 99%

A Multigene Family of Trypsin/α-Amylase Inhibitors from Cereals

Carbonero¹,

Garcı́a-Olmedo²

1999

Seed Proteins

View full text Add to dashboard Cite

“…More than 10 homologous cDNA clones encoding the 14-16 kDa allergens have been obtained so far [8], indicating that the 14-16 kDa allergens are multigene products. DNA sequencing revealed that these cDNA clones all encoded proteins similar to wheat a-amylase inhibitor, barley trypsin inhibitor and the castor bean storage protein [9], which had been identified as major allergenic proteins associated with baker's asthma [10,11].…”

Section: Introductionmentioning

confidence: 99%

Reduction of 14–16 kDa allergenic proteins in transgenic rice plants by antisense gene

et al. 1996

View full text Add to dashboard Cite

An antisense gene strategy was applied to suppress the 14-16 kDa allergen gene expression in maturing rice seeds. Gene constructs producing antisense RNAs of the 16 kDa allergen under the control of some rice seed-specific promoters were introduced into rice by electroporation. Immunoblot and RNA blot analyses of the seeds from the transgenic rice plants using the allergen-specific monoclonal antibody and a sequencespecific antisense RNA probe demonstrated that the 14-16 kDa allergen proteins and their transcripts of the seeds from several transgenic lines were present in much lower in amounts than those of the seeds from parental wild-type rice. The high levels of reduction observed were stably inherited in at least three generations.Key words: Rice allergen; Transgenic rice; Antisense RNA has been used experimentally to inhibit gene expression in bacteria, yeast, plant and animal cells [12], and the antisense strategy has also been reported to be practically applicable to transgenic crop plants, to which genes that produce antisense RNA are introduced to suppress the gene expression [13,14]. In the present study, the antisense genes for the 16 kDa allergen were introduced into rice by electroporation, and specific suppression of the 14-16 kDa allergen gene expression, resulting in the reduction of corresponding allergenic proteins in the mature seeds, was examined for regenerated transgenic rice plants and their progeny. The results show that antisense RNA markedly reduced the mRNA and protein contents of the 14-16 kDa allergens in the transgenic rice seeds. Materials and methods

show abstract

“…2) were found for trypsin inhibitors BTI and RTI [25,26], as well as rye c~-amylase inhibitor-3 and its wheat (WTAI-CM2) and barley (BTAI-CMa) homologues [16]. Interestingly, major allergens from barley (BMAI [8,18]) and rice (RAP [11,12]) also showed significant sequence identity (>35%).…”

Section: Sec C 1 and Bdp Are Related To The Cereal ~-Amylase/trypsin mentioning

confidence: 99%

“…The best characterized wheat and barley flour allergens are 12-16 kDa proteins that belong to the cereal a-amylase/trypsin inhibitor family [8][9][10]. Most members of this family show IgE binding capacity in vitro and cause positive responses in skin prick tests.…”

Section: Introductionmentioning

confidence: 99%

A major baker's asthma allergen from rye flour is considerably more active than its barley counterpart

García‐Casado¹,

Armentia²,

Sánchez‐Monge³

et al. 1995

FEBS Letters

Self Cite

View full text Add to dashboard Cite

A rye flour protein of about 13.5 kDa, as well as its barley homologue, have been isolated. The rye component was recognized in vitro by IgE of allergic patients and provoked positive responses in 15 out of 21 baker's asthma patients (71%) when skin prick tests were performed. Its barley homologue showed no detectable in vitro reactivity and caused positive responses in only one-third of patients. Although no inhibitory activity against different a-amylases or trypsin was found for these two proteins, their N-terminal sequencing revealed considerable similarity to several members of the cereal ~x-amylase/trypsin inhibitor family.

show abstract

A barley flour inhibitor of insect α‐amylase is a major allergen associated with baker's asthma disease

Cited by 74 publications

References 14 publications

A Multigene Family of Trypsin/α-Amylase Inhibitors from Cereals

A Multigene Family of Trypsin/α-Amylase Inhibitors from Cereals

Reduction of 14–16 kDa allergenic proteins in transgenic rice plants by antisense gene

A major baker's asthma allergen from rye flour is considerably more active than its barley counterpart

Contact Info

Product

Resources

About