A Basic Method for Hoechst (33258) Staining of Nuclei from Whole Root Tissues of Oryza sativa

Majumdar, Snehalata; Guha, Titir; Barman, Falguni; Kundu, Rita

doi:10.1007/s40009-019-00865-3

Cited by 4 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…, UVC light irradiation), the intensity of Hoechst 32258 fluorescence decreases, demonstrating the dsDNA unwinding. 47,62–64 The Hoechst 32258 fluorescence intensity was shown to decrease with a decrease in the concentration of dsDNA (ESI 4, Fig. S15a†).…”

Section: Resultsmentioning

confidence: 99%

“…, hyperchromicity effect of dsDNA and fluorometric analysis of DNA unwinding (FADU) assay). 46,47 Along with that, the NIR-II DCL imaging capability of these NCs for both steady-state and time-gated imaging modes and the possibility of NIR-II luminescence bioimaging through biological tissues (bovine tooth and chicken flesh) were also demonstrated.…”

Section: Introductionmentioning

confidence: 83%

“…As a proof-of-concept, we demonstrate the denaturation of double-stranded DNA (dsDNA) into single-stranded ones (DNA unwinding) caused by UVC emission generated by Pr 3+ /Yb 3+ doped NCs excited using a 447 nm laser, which was confirmed by two well-established independent experimental methods (i.e., hyperchromicity effect of dsDNA and fluorometric analysis of DNA unwinding (FADU) assay). 46,47 Along with that, the NIR-II DCL imaging capability of these NCs for both steady-state and time-gated imaging modes and the possibility of NIR-II luminescence bioimaging through biological tissues (bovine tooth and chicken flesh) were also demonstrated.…”

Section: Introductionmentioning

confidence: 85%

See 2 more Smart Citations

Pr³⁺ doped NaYF₄ and LiYF₄ nanocrystals combining visible-to-UVC upconversion and NIR-to-NIR-II downconversion luminescence emissions for biomedical applications

et al. 2022

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 85%

See 1 more Smart Citation

Pr³⁺ doped NaYF₄ and LiYF₄ nanocrystals combining visible-to-UVC upconversion and NIR-to-NIR-II downconversion luminescence emissions for biomedical applications

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Hoechst [75]. These dyes are unaffected by chromatin condensation because they attach to DNA in a sequence-specific manner.…”

Section: Stainingmentioning

confidence: 99%

“…As a result, they are not the best choice for relative DNA measures, such as determining ploidy levels. Relative DNA measurements are better suited to fluorescent dyes with AT‐specificity, particularly 4′,6‐diamidino‐2‐phenylindole (DAPI) [74] and Hoechst [75]. These dyes are unaffected by chromatin condensation because they attach to DNA in a sequence‐specific manner.…”

Section: Isolation Of Plant Nucleimentioning

confidence: 99%

Unveiling the epigenetic landscape of plants using flow cytometry approach

Jyoti,

Chandel,

Singh

2024

Cytometry Pt A

View full text Add to dashboard Cite

Plants are sessile creatures that have to adapt constantly changing environmental circumstances. Plants are subjected to a range of abiotic stressors as a result of unpredictable climate change. Understanding how stress‐responsive genes are regulated can help us better understand how plants can adapt to changing environmental conditions. Epigenetic markers that dynamically change in response to stimuli, such as DNA methylation and histone modifications are known to regulate gene expression. Individual cells or particles' physical and/or chemical properties can be measured using the method known as flow cytometry. It may therefore be used to evaluate changes in DNA methylation, histone modifications, and other epigenetic markers, making it a potent tool for researching epigenetics in plants. We explore the use of flow cytometry as a technique for examining epigenetic traits in this thorough discussion. The separation of cell nuclei and their subsequent labeling with fluorescent antibodies, offering information on the epigenetic mechanisms in plants when utilizing flow cytometry. We also go through the use of high‐throughput data analysis methods to unravel the complex epigenetic processes occurring inside plant systems.

show abstract

Fabrication and Characterization of Biomimetic Electrospun Cartilage Decellularized Matrix (CDM)/Chitosan Nanofiber Hybrid for Tissue Engineering Applications: Box-Behnken Design for Optimization

saberi,

Khodaverdi,

Kamali

et al. 2023

J Polym Environ

View full text Add to dashboard Cite

A Basic Method for Hoechst (33258) Staining of Nuclei from Whole Root Tissues of Oryza sativa

Cited by 4 publications

References 15 publications

Pr³⁺ doped NaYF₄ and LiYF₄ nanocrystals combining visible-to-UVC upconversion and NIR-to-NIR-II downconversion luminescence emissions for biomedical applications

Pr³⁺ doped NaYF₄ and LiYF₄ nanocrystals combining visible-to-UVC upconversion and NIR-to-NIR-II downconversion luminescence emissions for biomedical applications

Unveiling the epigenetic landscape of plants using flow cytometry approach

Fabrication and Characterization of Biomimetic Electrospun Cartilage Decellularized Matrix (CDM)/Chitosan Nanofiber Hybrid for Tissue Engineering Applications: Box-Behnken Design for Optimization

Contact Info

Product

Resources

About

A Basic Method for Hoechst (33258) Staining of Nuclei from Whole Root Tissues of Oryza sativa

Cited by 4 publications

References 15 publications

Pr3+ doped NaYF4 and LiYF4 nanocrystals combining visible-to-UVC upconversion and NIR-to-NIR-II downconversion luminescence emissions for biomedical applications

Pr3+ doped NaYF4 and LiYF4 nanocrystals combining visible-to-UVC upconversion and NIR-to-NIR-II downconversion luminescence emissions for biomedical applications

Unveiling the epigenetic landscape of plants using flow cytometry approach

Fabrication and Characterization of Biomimetic Electrospun Cartilage Decellularized Matrix (CDM)/Chitosan Nanofiber Hybrid for Tissue Engineering Applications: Box-Behnken Design for Optimization

Contact Info

Product

Resources

About

Pr³⁺ doped NaYF₄ and LiYF₄ nanocrystals combining visible-to-UVC upconversion and NIR-to-NIR-II downconversion luminescence emissions for biomedical applications

Pr³⁺ doped NaYF₄ and LiYF₄ nanocrystals combining visible-to-UVC upconversion and NIR-to-NIR-II downconversion luminescence emissions for biomedical applications