Silver nanoparticle (Ag-np) was successfully synthesized by using the simple green chemistry method. Its antibacterial activity was studied against Staphylococcus aureus and Escherichia coli according to agar well diffusion method. The green synthesis was carried out by mixing the silver nitrate, glucose, and starch as precursors at 90 0 C for 4 hours. The UV-Vis spectra show the characteristic of spherical Ag-np in the range of 410-425 nm and it is confirmed by TEM image. The Ag-np has an average diameter of 8.41 ± 4.37 nm. As the reductant mole composition increases, the SPR decreases and shifts to red due to the further growth of the Ag-np. When the stabilizer composition increases, the SPR intensity also increases and shifts to red. The formed Ag-np is stable even after one-month synthesis. The antibacterial test on the Ag-np was carried out against gram-positive bacteria (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli). The result shows that a higher concentration of Ag-np is required to kill Staphylococcus aureus compared to Escherichia coli.